The Role of Bacterial Chaperones in the Circulative Transmission of Plant Viruses by Insect Vectors

Kliot, Adi; Ghanim, Murad

doi:10.3390/v5061516

Cited by 28 publications

(19 citation statements)

References 95 publications

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“…Our quantification of the viral loads and DNA complementary strands of both Mld and IL strains of TYLCV in MEAM1 B. tabaci during non-acquisition access periods points to an effective preservation mechanism, rather than to substantial replication to compensate for degradation. Our results reinforce the “transmissible reservoir” hypothesis in the case of the circulative non-propagative transmission mode of viruses by insect vectors 10 12 18 32 34 . A repertoire of B. tabaci chaperonins 35 might be involved in this long-term conservation (HSP70 36 37 or BtHSP16 38 ) and transmission (GroEL chaperonins encoded by secondary endosymbionts of B. tabaci , such as Hamiltonella sp.…”

Section: Resultssupporting

confidence: 84%

Rapid accumulation and low degradation: key parameters of Tomato yellow leaf curl virus persistence in its insect vector Bemisia tabaci

Becker

Rimbaud

Chiroleu

et al. 2015

Sci Rep

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Of worldwide economic importance, Tomato yellow leaf curl virus (TYLCV, Begomovirus) is responsible for one of the most devastating plant diseases in warm and temperate regions. The DNA begomoviruses (Geminiviridae) are transmitted by the whitefly species complex Bemisia tabaci. Although geminiviruses have long been described as circulative non-propagative viruses, observations such as long persistence of TYLCV in B. tabaci raised the question of their possible replication in the vector. We monitored two major TYLCV strains, Mild (Mld) and Israel (IL), in the invasive B. tabaci Middle East-Asia Minor 1 cryptic species, during and after the viral acquisition, within two timeframes (0–144 hours or 0–20 days). TYLCV DNA was quantified using real-time PCR, and the complementary DNA strand of TYLCV involved in viral replication was specifically quantified using anchored real-time PCR. The DNA of both TYLCV strains accumulated exponentially during acquisition but remained stable after viral acquisition had stopped. Neither replication nor vertical transmission were observed. In conclusion, our quantification of the viral loads and complementary strands of both Mld and IL strains of TYLCV in B. tabaci point to an efficient accumulation and preservation mechanism, rather than to a dynamic equilibrium between replication and degradation.

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Section: Resultssupporting

confidence: 84%

Rapid accumulation and low degradation: key parameters of Tomato yellow leaf curl virus persistence in its insect vector Bemisia tabaci

Becker

Rimbaud

Chiroleu

et al. 2015

Sci Rep

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“…Examples include Potato leafroll virus transmission by the green peach aphid Myzus persicae and Tomato yellow leaf curl virus transmission by the sweetpotato whitefly Bemisia tabaci . In these cases, the viruses gain protection and achieve successful transmission by binding to GroEL, a hemolymph protein produced by endosymbiotic bacteria of the insect [ 52 – 54 ]. During the transmission of West Nile Virus by Aedes aegypti , viruses can even establish infection in hemocytes by binding to the mosquito hemolymph protein that leads to the phagocytosis pathway [ 55 ].…”

Section: Discussionmentioning

confidence: 99%

Insect tissue-specific vitellogenin facilitates transmission of plant virus

Huo

Chen

et al. 2018

PLoS Pathog

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Insect vitellogenin (Vg) has been considered to be synthesized in the fat body. Here, we found that abundant Vg protein is synthesized in Laodelphax striatellus hemocytes as well. We also determined that only the hemocyte-produced Vg binds to Rice stripe virus (RSV) in vivo. Examination of the subunit composition of L. striatellus Vg (LsVg) revealed that LsVg was processed differently after its expression in different tissues. The LsVg subunit able to bind to RSV exist stably only in hemocytes, while fat body-produced LsVg lacks the RSV-interacting subunit. Nymph and male L. striatellus individuals also synthesize Vg but only in hemocytes, and the proteins co-localize with RSV. We observed that knockdown of LsVg transcripts by RNA interference decreased the RSV titer in the hemolymph, and thus interfered with systemic virus infection. Our results reveal the sex-independent expression and tissue-specific processing of LsVg and also unprecedentedly connect the function of this protein in mediating virus transmission to its particular molecular forms existing in tissues previously known as non-Vg producing.

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“…Expression of Hsp70 can be induced by the presence of mutant misfolded coat protein of tobacco mosaic virus rather than the wild-type CP 33 . The role of GroEL chaperonins in the transmission efficiency of TYLCV has also been well illustrated 34 , however, their potential role in virus mutation needs to be further investigated.…”

Section: Discussionmentioning

confidence: 99%

Molecular variation of tomato yellow leaf curl virus in the insect vector Bemisia tabaci

Yang

Luan

et al. 2017

Sci Rep

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Insect vectors play significant roles in geminivirus spread and evolution in nature. To date little is known about the population dynamics of begomoviruses in their insect vector Bemisia tabaci. In this study we analyzed the genetic variation of tomato yellow leaf curl virus (TYLCV) in its host plant, Solanum lycopersicum, in its transmission vector B. tabaci raised on TYLCV-infected S. lycopersicum plants, and in B. tabaci after being transferred from S. lycopersicum to Gossypium hirsutum. We found that the levels of variability of TYLCV remained stable in S. lycopersicum plants, but increased significantly in both invasive and indigenous species of B. tabaci. We also presented evidence that the elevated mutation frequencies in TYLCV populations from vector whiteflies were caused mainly by mutations that occurred at several distinct sites within the TYLCV genome. Simultaneous introduction of mutations in the hot spots did not affect the ability of TYLCV to be transmitted by B. tabaci, but reduced its pathogenicity in both S. lycopersicum and Nicotiana benthamiana. Our findings provide new information on population variability of TYLCV in its insect vector, extending the knowledge of the influence of insect vector on plant virus population dynamics.

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The Role of Bacterial Chaperones in the Circulative Transmission of Plant Viruses by Insect Vectors

Cited by 28 publications

References 95 publications

Rapid accumulation and low degradation: key parameters of Tomato yellow leaf curl virus persistence in its insect vector Bemisia tabaci

Rapid accumulation and low degradation: key parameters of Tomato yellow leaf curl virus persistence in its insect vector Bemisia tabaci

Insect tissue-specific vitellogenin facilitates transmission of plant virus

Molecular variation of tomato yellow leaf curl virus in the insect vector Bemisia tabaci

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