The Role of Background Bacteria in Preventing the Isolation and Detection of Shigella and STEC in Foods and Novel MuSIC ddPCR Method to Improve STEC Detection in the Presence of Background Bacteria
Abstract:Foodborne illness caused by Enterohemorrhagic E. coli (EHEC) and Shigella remain a public health issue in developed countries. The current microbiological methods for detecting EHEC and Shigella typically involve an enrichment step that aims to amplify the pathogenic organisms relative to the background bacteria flora present in foods. Following enrichment, there is a screening step to identify pathogens based on presence of virulence genes (ex. stx and eae for EHEC). False-positive detection of EHEC can occur… Show more
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