The role of B cells in an early immune response to Mycobacterium bovis

Kročová, Zuzana; Plzakova, Lenka; Pávková, Ivona; Kubelková, Klára; Macela, Aleš; Ožanič, Mateja; Marečić, Valentina; Mihelčić, Mirna; Šantić, Marina

doi:10.1016/j.micpath.2019.103937

Cited by 11 publications

(6 citation statements)

References 28 publications

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“…The number of B lymphocytes observed was higher in the BCG group and lower in the HIMB-vaccinated animals, compared to the control group. B cells are involved in the host's early interactions with intracellular bacterial pathogens, and also participate in the induction of innate defense responses [47]. We found more B lymphocytes in BCG vaccinated badgers than in control animals, similar to what was observed in previous studies in cattle [34].…”

Section: Discussionsupporting

confidence: 89%

Local Lung Immune Response to Mycobacterium bovis Challenge after BCG and M. bovis Heat-Inactivated Vaccination in European Badger (Meles meles)

et al. 2020

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Tuberculosis (TB) vaccination could be used as a key part of integrated strategies for the disease’s control if an effective and safe vaccine under field conditions is obtained. Recent studies in Spain have evaluated the protective efficacy of two oral vaccines against experimental challenge with live intra-bronchial Mycobacterium bovis in captive badgers: the live-attenuated M. bovis BCG vaccine (Danish strain) and a heat-inactivated M. bovis (HIMB) vaccine. With the objective of increasing the knowledge of the cellular development progress of infection and generating further tools to discriminate between mild and severe TB lesions between and within animals, the immunopathology of tuberculous lesions was studied to characterize the local immune response (cell type profile) within lung granulomas from control (non-vaccinated), BCG vaccinated and HIMB-vaccinated experimentally infected badgers with M. bovis. Four immunohistochemical protocols, for the specific detection of macrophages, T lymphocytes, B lymphocytes and plasma cells within TB granulomas in formalin fixed sections of the right middle lung lobe (lobe targeted for the M. bovis delivery), were performed. Immunolabelled sections were scanned and five randomly selected areas were analyzed with digital image analysis software. The results were expressed as the proportion of the positively immunolabelled area within the total area of the selected site. Data was analyzed using the statistical analysis software (SAS). In the three treatment groups, macrophages were the most abundant inflammatory cells within the granulomas, followed by B lymphocytes and plasma cells. T lymphocyes were absent in those granulomas. This would suggest a predominance of a non-specific innate response mediated by phagocytic cells over an adaptative humoral immune response. The proportion of macrophages and plasma cells was higher in BCG and HIMB-vaccinated badgers, respectively, suggesting the establishment of an adaptative humoral response in HIMB-vaccinated badgers. The lower bacterial load at the lung level, as well as the volume of lesions in lungs using magnetic resonance imaging in badgers with the HIMB vaccine in relation with local immune response presented, must be highlighted, since it would be an advantage in favor of its use under field conditions in terms of reducing TB transmission and environmental contamination.

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Section: Discussionsupporting

confidence: 89%

Local Lung Immune Response to Mycobacterium bovis Challenge after BCG and M. bovis Heat-Inactivated Vaccination in European Badger (Meles meles)

et al. 2020

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“…Instead, our data suggest that the major IFNγ producers are restricted to a B-like cell population. Intriguingly, a similar subpopulation of innate B cells producing IFNγ has been reported to be vital in regulating and initiating early innate immune response of intracellular bacterial infections (Bao et al, 2014;Krocova et al, 2020). In addition, other prior works revealed that mature B cells, when primed by T cells and stimulated by pathogens or TLR ligands, can secrete IFNγ (Gray et al, 2007;Lund & Randall, 2010).…”

Section: Discussionmentioning

confidence: 95%

Renal CD169⁺⁺resident macrophages are crucial for protection against acute systemic candidiasis

Teo

Mak

et al. 2021

Life Sci. Alliance

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Disseminated candidiasis remains as the most common hospital-acquired bloodstream fungal infection with up to 40% mortality rate despite the advancement of medical and hygienic practices. While it is well established that this infection heavily relies on the innate immune response for host survival, much less is known for the protective role elicited by the tissue-resident macrophage (TRM) subsets in the kidney, the prime organ for Candida persistence. Here, we describe a unique CD169++ TRM subset that controls Candida growth and inflammation during acute systemic candidiasis. Their absence causes severe fungal-mediated renal pathology. CD169++ TRMs, without being actively involved in direct fungal clearance, increase host resistance by promoting IFN-γ release and neutrophil ROS activity.

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“…These epitopes of B-cells and T-cells may theoretically be used to produce vaccines targeting Mtb and may be reliable for stimulating both humoral and cell-mediated immunity. Prediction of B-cell epitopes is a significant characteristic for the designing of vaccines 70 , and to provide the site for the antigen-antibody interactions [71][72][73] . In the present research, 16-mer B-cell epitopes were predicted via the ABCpred server.…”

Section: Discussionmentioning

confidence: 99%

In silico analysis of epitope-based vaccine candidate against tuberculosis using reverse vaccinology

Bibi

Ullah

Zhu

et al. 2021

Sci Rep

108

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Tuberculosis (TB) kills more individuals in the world than any other disease, and a threat made direr by the coverage of drug-resistant strains of Mycobacterium tuberculosis (Mtb). Bacillus Calmette–Guérin (BCG) is the single TB vaccine licensed for use in human beings and effectively protects infants and children against severe military and meningeal TB. We applied advanced computational techniques to develop a universal TB vaccine. In the current study, we select the very conserved, experimentally confirmed Mtb antigens, including Rv2608, Rv2684, Rv3804c (Ag85A), and Rv0125 (Mtb32A) to design a novel multi-epitope subunit vaccine. By using the Immune Epitopes Database (IEDB), we predicted different B-cell and T-cell epitopes. An adjuvant (Griselimycin) was also added to vaccine construct to improve its immunogenicity. Bioinformatics tools were used to predict, refined, and validate the 3D structure and then docked with toll-like-receptor (TLR-3) using different servers. The constructed vaccine was used for further processing based on allergenicity, antigenicity, solubility, different physiochemical properties, and molecular docking scores. The in silico immune simulation results showed significant response for immune cells. For successful expression of the vaccine in E. coli, in-silico cloning and codon optimization were performed. This research also sets out a good signal for the design of a peptide-based tuberculosis vaccine. In conclusion, our findings show that the known multi-epitope vaccine may activate humoral and cellular immune responses and maybe a possible tuberculosis vaccine candidate. Therefore, more experimental validations should be exposed to it.

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The role of B cells in an early immune response to Mycobacterium bovis

Cited by 11 publications

References 28 publications

Local Lung Immune Response to Mycobacterium bovis Challenge after BCG and M. bovis Heat-Inactivated Vaccination in European Badger (Meles meles)

Local Lung Immune Response to Mycobacterium bovis Challenge after BCG and M. bovis Heat-Inactivated Vaccination in European Badger (Meles meles)

Renal CD169⁺⁺resident macrophages are crucial for protection against acute systemic candidiasis

In silico analysis of epitope-based vaccine candidate against tuberculosis using reverse vaccinology

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The role of B cells in an early immune response to Mycobacterium bovis

Cited by 11 publications

References 28 publications

Local Lung Immune Response to Mycobacterium bovis Challenge after BCG and M. bovis Heat-Inactivated Vaccination in European Badger (Meles meles)

Local Lung Immune Response to Mycobacterium bovis Challenge after BCG and M. bovis Heat-Inactivated Vaccination in European Badger (Meles meles)

Renal CD169++resident macrophages are crucial for protection against acute systemic candidiasis

In silico analysis of epitope-based vaccine candidate against tuberculosis using reverse vaccinology

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Renal CD169⁺⁺resident macrophages are crucial for protection against acute systemic candidiasis