The role of auxin in developmentally regulated programmed cell death in lace plant

Denbigh, Georgia L.; Dauphinee, Adrian N.; Fraser, Meredith S.; Lacroix, Christian; Gunawardena, Arunika H. L. A. N.

doi:10.1002/ajb2.1463

Cited by 10 publications

(9 citation statements)

References 55 publications

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“…Phytohormone signal transduction is likely the prevailing mechanism responsible for upstream regulation of PCD processes [7]. For example, ethylene is responsible for leaf senescence and root deterioration in maize [11,12]; auxin is responsible for developmentally-regulated PCD in lace plant [32]; GA is related to both aleuronal and tapetal PCD [14,15]; the combination of auxin, cytokinin, and BR are related to PCD of TEs [33]; and JA and SA are related to both aerenchyma formation and leaf senescence [18,19].…”

Section: The Role Of the Ethylene Signaling In Replaceable Bud Pcd Of...mentioning

confidence: 99%

A Transcriptomic Evaluation of the Mechanism of Programmed Cell Death of the Replaceable Bud in Chinese Chestnut

Guo¹,

Zhang²,

Liu³

et al. 2023

Preprint

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Previous research suggests that the senescence and death of the replaceable bud in chestnut cultivar (cv.) ‘Tima Zhenzhu’ involves programmed cell death (PCD). However, the molecular network responsible for regulating replaceable bud PCD is poorly characterized. Here, we performed transcriptomic profiling of the chestnut cv. ‘Tima Zhenzhu’ replaceable bud before (S20), during (S25), and after PCD (S30) to ascertain the molecular mechanism underlying the PCD process. A total of 5,779, 9,867, and 2,674 differentially expressed genes (DEGs) were discovered upon comparison of S20 vs. S25, S20 vs. S30, and S25 vs. S30, respectively. Approximately 6,137 DEGs common to at least two comparisons were selected for GO and KEGG enrichment analyses to interrogate the main corresponding biological functions and pathways. GO analysis showed that these common DEGs could be divided into three functional categories, including 15 cellular components, 14 molecular functions, and 19 biological processes. KEGG analysis found that “plant hormone signal transduction” included 93 DEGs. Overall, 441 DEGs were identified as related to the process of PCD. Most of these were found to be genes associated with ethylene signaling, as well as initiation and execution of various PCD processes. A hypothetical model, consisting of three overlapping processes, is proposed for the replaceable bud PCD: First, ethylene signaling is activated during preparation for PCD, in order to regulate the activity of downstream targets. Next, during PCD initiation, the up-regulation of several TFs (including MYB, MADS-box, bHLH, and NAC TFs) induces an increase in cytochrome c expression and in the cytosolic Ca2+ content, activating the Ca2+-dependent signaling cascade. Finally, during PCD execution, the process of autophagy and the activity of proteases (i.e., cysteine proteinases RD21A-like, metacaspase-9-like, vacuolar-processing enzyme-like, and senescence-associated proteins for hydrolysis) work synergistically to clear the cell of cellular components. When this process is complete, the replaceable bud senesces and dies.

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Section: The Role Of the Ethylene Signaling In Replaceable Bud Pcd Of...mentioning

confidence: 99%

A Transcriptomic Evaluation of the Mechanism of Programmed Cell Death of the Replaceable Bud in Chinese Chestnut

Guo¹,

Zhang²,

Liu³

et al. 2023

Preprint

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“…A recent study provided evidence for the involvement of auxin signalling in another type of developmental plant PCD programme. Denbigh et al [31] convincingly showed the role of auxin in the induction and/or execution of developmental PCD during leaf morphogenesis in lace plant [31]. PCD modulates lace plant leaf development by producing distinct perforations between longitudinal and transverse veins, offering an unique and elegant model system for studying the timing and regulation of the cell death process [96].…”

Section: To Die or Not To Die? Auxin-mediated Modulation Of Plant Pcd...mentioning

confidence: 99%

“…PCD modulates lace plant leaf development by producing distinct perforations between longitudinal and transverse veins, offering an unique and elegant model system for studying the timing and regulation of the cell death process [96]. Chemical inhibition of auxin transport and signalling, at levels not affecting the plant growth itself, prevented the hallmark PCD features, such as release of mitochondrial cytochrome c and ROS accumulation, and resulted in fewer, to no, perforations [31]. The spatio‐temporal tracking of auxin gradients during lace plant leaf perforation formation, for example, by using immunolocalization, would provide further insights into how auxin regulates this type of developmental PCD.…”

Section: To Die or Not To Die? Auxin‐mediated Modulation Of Plant Pcd...mentioning

confidence: 99%

“…However, our understanding of the intimate relationship between this key phytohormone and cell death regulation in plants has increased substantially thanks to research undertaken in the last decade, and many mutants have been identified that display both auxin response, and altered-PCD, phenotypes (Table 1). Interestingly, while auxin activity can regulate the PCD pathway in numerous contexts [31,32], recent discoveries in the field suggest that this regulation is bidirectional and localized PCD events can modulate auxin flux and signalling, consequently having a targeted effect on tissue regeneration and plant development [33][34][35]. This suggests intriguing insights into the specific roles of auxin-PCD interplay in many finely tuned plant responses and developmental processes (Fig.…”

Section: Introductionmentioning

confidence: 99%

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Plant programmed cell death meets auxin signalling

et al. 2021

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Both auxin signalling and programmed cell death (PCD) are essential components of a normally functioning plant. Auxin underpins plant growth and development, as well as regulating plant defences against environmental stresses. PCD, a genetically controlled pathway for selective elimination of redundant, damaged or infected cells, is also a key element of many developmental processes and stress response mechanisms in plants. An increasing body of evidence suggests that auxin signalling and PCD regulation are often connected. While generally auxin appears to suppress cell death, it has also been shown to promote PCD events, most likely via stimulation of ethylene biosynthesis. Intriguingly, certain cells undergoing PCD have also been suggested to control the distribution of auxin in plant tissues, by either releasing a burst of auxin or creating an anatomical barrier to auxin transport and distribution. These recent findings indicate novel roles of localized PCD events in the context of plant development such as control of root architecture, or tissue regeneration following injury, and suggest exciting possibilities for incorporation of this knowledge into crop improvement strategies.

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“…This pigmentation disappears as leaves mature and chlorophyll pigmentation becomes more prominent (Figure 1C). The lace plant is an excellent model system for studying developmentally regulated PCD in planta due to the accessibility and predictability of perforation formation, the suitability of its thin and semi-transparent leaves for live cell imaging, and the established sterile culture system for propagation [3][4][5][6][7][8][9][10].…”

Section: Introductionmentioning

confidence: 99%

In vitro Anticancer Activity of Aponogeton madagascariensis Anthocyanin Extracts

Gunawardena

Rollini

Rasmussen³

et al. 2021

J NHP Res

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INTRODUCTION:Aponogeton madagascariensis (lace plant) is a freshwater aquatic flowering plant belonging to the family Aponogetonaceae that forms leaf perforations via programmed cell death (PCD). The lace plant has emerged as a novel model system for studying PCD in planta due to the predictability and accessibility of this process. Anthocyanins, and the balance between ROS and antioxidants, play a central role in regulating PCD in lace plant leaves. Aponogetonaceae family members have shown medicinal properties, including antioxidant and anticancer activities; however, nothing is known about the lace plant's potential for medicinal use. Therefore, this study evaluated the anticancer activities of lace plant anthocyanin extracts. METHODS:Cell line growth and viability were assessed following exposure to lace plant leaf anthocyanin extracts. This study utilized a triple-negative breast cancer cell line, MDA-MB-231, two human ovarian epithelial cancer cell lines, OVCAR-8 and SKOV-3, along with a normal mammary epithelial cell line, MCF-10A. Furthermore, crude anthocyanin extracts were fractionated into anthocyanin and non-anthocyanin containing fractions and tested only on MDA-MB-231 cells. RESULTS AND DISCUSSION:The crude anthocyanin extracts from lace plant leaves inhibited the growth of MDA-MB-231, OVCAR-8, and SKOV-3 cells in a concentration-dependent manner and had no effect on MCF-10A cells. Lace plant crude anthocyanin extracts appeared to induce apoptosis in MDA-MB-231 cells. Interestingly, treatment with anthocyanin and non-anthocyanin fractions decreased the growth of MDA-MB-231, similarly to crude anthocyanin extracts, suggesting the presence of other anticancer compounds in the lace plant extracts.

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The role of auxin in developmentally regulated programmed cell death in lace plant

Cited by 10 publications

References 55 publications

A Transcriptomic Evaluation of the Mechanism of Programmed Cell Death of the Replaceable Bud in Chinese Chestnut

A Transcriptomic Evaluation of the Mechanism of Programmed Cell Death of the Replaceable Bud in Chinese Chestnut

Plant programmed cell death meets auxin signalling

In vitro Anticancer Activity of Aponogeton madagascariensis Anthocyanin Extracts

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