The role of autophagy induction in the mechanism of cytoprotective effect of resveratrol

Ulakcsai, Zsófia; Bagaméry, Fruzsina; Szökő, Éva; Tábi, Tamás

doi:10.1016/j.ejps.2018.07.039

Cited by 11 publications

(9 citation statements)

References 48 publications

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“…Resveratrol is another important polyphenol frequently studied for its beneficial effect in increasing longevity and balances cellular protein homeostasis. The activation of SIRT1/AMPK and extracellular signal-regulated kinases (ERK1/2) is the molecular mechanism by which this polyphenol was found to be neuroprotective [9,303,304]. The metabolite of ellagitannin, urolithin A, extracted from pomegranate has been reported to activate autophagy through SIRT1 activation [305].…”

Section: Multiple Targets Of Polyphenols Against Admentioning

confidence: 99%

Dietary Polyphenols: A Multifactorial Strategy to Target Alzheimer’s Disease

Dhakal

Kushairi

Phan

et al. 2019

IJMS

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Ageing is an inevitable fundamental process for people and is their greatest risk factor for neurodegenerative disease. The ageing processes bring changes in cells that can drive the organisms to experience loss of nutrient sensing, disrupted cellular functions, increased oxidative stress, loss of cellular homeostasis, genomic instability, accumulation of misfolded protein, impaired cellular defenses and telomere shortening. Perturbation of these vital cellular processes in neuronal cells can lead to life threatening neurological disorders like Alzheimer’s Disease, Parkinson’s Disease, Huntington’s Disease, Lewy body dementia, etc. Alzheimer’s Disease is the most frequent cause of deaths in the elderly population. Various therapeutic molecules have been designed to overcome the social, economic and health care burden caused by Alzheimer’s Disease. Almost all the chemical compounds in clinical practice have been found to treat symptoms only limiting them to palliative care. The reason behind such imperfect drugs may result from the inefficiencies of the current drugs to target the cause of the disease. Here, we review the potential role of antioxidant polyphenolic compounds that could possibly be the most effective preventative strategy against Alzheimer’s Disease.

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Section: Multiple Targets Of Polyphenols Against Admentioning

confidence: 99%

Dietary Polyphenols: A Multifactorial Strategy to Target Alzheimer’s Disease

Dhakal

Kushairi

Phan

et al. 2019

IJMS

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“…Studies have shown that resveratrol-induced autophagy or apoptosis regulates brosis in multiple types of broblasts, including broblast-like synovial cells [30], primary mouse embryonic broblasts [31],…”

Section: Resveratrol Inhibits Hsc Activation By Inducing Autophagy An...mentioning

confidence: 99%

Resveratrol inhibits hepatic stellate cell activation by regulating autophagy and apoptosis through the SIRT1 and JNK signaling pathways

Zhang

Jian

Jiang

et al. 2022

Journal of Food Biochemistry

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Resveratrol, a natural polyphenol found in grapes, berries, peanuts, and medicinal plants, has previously been reported to have several biological activities, including inhibiting hepatic brosis. Hepatic stellate cell (HSC) activation is the major cellular source of matrix protein-secreting myo broblasts, which are the major drivers of liver brogenesis. Numerous studies on the protective effects of resveratrol against liver brosis have focused on the inhibition of HSC activation. Although the underlying mechanisms remain to be fully elucidated, autophagy and apoptosis regulation might be intimately related. The mouse HSC line JS1 was stimulated with resveratrol to assess the mechanism and relationship between autophagy and apoptosis. Resveratrol dose-dependently modulated JS1 cell viability. Moreover, resveratrol inhibited JS1 cell activation and induced autophagy and apoptosis. This anti brotic effect was attenuated when autophagy was inhibited using chloroquine (CQ) or 3-methyladenine (3-MA) or when apoptosis was inhibited using Z-VAD-FMK. Furthermore, whether the Sirtuin1 (SIRT1) and c-Jun N-terminal kinase (JNK) signaling pathways were associated with resveratrol-induced autophagy and apoptosis in JS1 cells was examined. The SIRT1 inhibitor EX527 reversed autophagy, and the JNK inhibitor SP600125 was able to reverse both autophagy and apoptosis induced by resveratrol. These ndings suggest that the SIRT1 and JNK signaling pathways may be involved in resveratrol-mediated inhibition of HSC activation by regulating autophagy and apoptosis. Materials And Methods Cells and cell cultureThe immortalized mouse HSC line JS1 was kindly provided by Professor Scott L. Friedman (Mt. Sinai School of Medicine, USA). JS1 cells were cultured in DMEM (Gibco; Thermo Fisher Scienti c, Inc.) supplemented with 10% FBS (Gibco; Thermo Fisher Scienti c, Inc.) and 1% penicillin/streptomycin at 5% CO 2 and 37°C. The medium was replaced with media supplemented with 0.5% FBS for 12 h prior to treatment. Different concentrations of resveratrol (cat. no. R5010; Sigma) was added to the medium and an incubated for different durations, according to the experimental design. JS1 cells were pretreated with 5 mM 3-methyladenine (3-MA; cat. no. M9281; Sigma-Aldrich; Merck KGaA), 30 µM chloroquine (CQ) diphosphate salt (cat. no. C6628; Sigma-Aldrich; Merck KGaA) or 20 µM Z-VAD-FMK (cat. no. C1202; Beyotime Institute of Biotechnology) for 2 h and 10 µM EX527 (cat. no. ab141506; Abcam) or 10 µM SP600125 (cat. no. ab120065; Abcam) for 1 h, followed by treatment with or without 50 µM resveratrol for 24 h. Each in vitro experiment was repeated three times. Measurement of JS1 cell viabilityCells were plated in plastic 96-well plates and incubated with 0.1, 1, 10, 50 or 100 µM resveratrol for 24 h. Subsequently, cell viability was assessed using an MTT Cell Proliferation and Cytotoxicity Assay kit (cat.

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“…RE and its derivatives are mainly found in grapes, peanuts, and knotweed. [18][19][20][21][22][23] Because of its antioxidant, anti-inammatory, anti-cancer and cardiovascular protection effects, it is widely used in medicine, food and other elds. As a derivative of stilbene, due to its functional phenolic hydroxyl group and rigid conjugated structure, it has also been extensively utilized in the eld of ame retardancy.…”

Section: Introductionmentioning

confidence: 99%