The role of arabidopsis AtFes1A in cytosolic Hsp70 stability and abiotic stress tolerance

Zhang, Jingxia; Wang, Cui; Yang, Chuanyan; Wang, Jiaying; Chen, Lei; Bao, Xinhe; Zhao, Yanxiu; Zhang, Hui; Liu, Jian

doi:10.1111/j.1365-313x.2010.04173.x

Cited by 70 publications

(66 citation statements)

References 38 publications

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“…This has for example been shown to be the case in early defense response, where the E3 ubiquitin ligase CYS, MET, PRO, and GLY PROTEIN 1 (CMPG1, AT1G66160), which itself is a target of the UPS, gets stabilized and accumulates (80). This also applies to the heat shock protein HSP70, as cytosolic HSP70 associates with FES1A (AT3G09350), which is induced by high temperatures and prevents HSP70 degradation (81). The level of FES1A was increased after SylA treatment (Table I), as were transcript levels for HSP70 and FES1A.…”

Section: Discussionmentioning

confidence: 99%

Protein Abundance Changes and Ubiquitylation Targets Identified after Inhibition of the Proteasome with Syringolin A

Svozil

Hirsch‐Hoffmann

Dudler

et al. 2014

Molecular & Cellular Proteomics

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As proteins are the main effectors inside cells, their levels need to be tightly regulated. This is partly achieved by specific protein degradation via the Ubiquitin-26S proteasome system (UPS). In plants, an exceptionally high number of proteins are involved in Ubiquitin-26S proteasome system-mediated protein degradation and it is known to regulate most, if not all, important cellular processes. Here, we investigated the response to the inhibition of the proteasome at the protein level treating leaves with the specific inhibitor Syringolin A (SylA) in a daytime specific manner and found 109 accumulated and 140 decreased proteins. The patterns of protein level changes indicate that the accumulating proteins cause proteotoxic stress that triggers various responses. Comparing protein level changes in SylA treated with those in a transgenic line over-expressing a mutated ubiquitin unable to form polyubiquitylated proteins produced little overlap pointing to different response pathways. To distinguish between direct and indirect targets of the UPS we also enriched and identified ubiquitylated proteins after inhibition of the proteasome, revealing a total of 1791 ubiquitylated proteins in leaves and roots and 1209 that were uniquely identified in our study. The comparison of the ubiquitylated proteins with those changing in abundance after SylA-mediated inhibition of the proteasome confirmed the complexity of the response and revealed that some proteins are regulated both at transcriptional and post-transcriptional level. For the ubiquitylated proteins that accumulate in the cytoplasm but are targeted to the plastid or the mitochondrion, we often found peptides in their target sequences, demonstrating that the UPS is involved in controlling organellar protein levels. Attempts to identify the sites of ubiquitylation revealed that the specific properties of this post-translational modification can lead to incorrect peptide spectrum assignments in complex peptide mixtures in which only a small fraction of peptides is expected to carry the ubiquitin footprint. This was confirmed with measurements of synthetically produced peptides and calculating the similarities between the different spectra. Molecular & Cellular

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Section: Discussionmentioning

confidence: 99%

Protein Abundance Changes and Ubiquitylation Targets Identified after Inhibition of the Proteasome with Syringolin A

Svozil

Hirsch‐Hoffmann

Dudler

et al. 2014

Molecular & Cellular Proteomics

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“…Therefore enhanced thermotolerance can also lead to enhanced salt tolerance. The Arabidopsis AtFes1A protein is involved in thermotolerance by stabilizing Hsp70 proteins, as shown in atfes1a knock out mutants, which had increased salt sensitivity and reduced thermotolerance [49]. This susceptibility was accounted for by increased ubiquitination of Hsp70 proteins, which are involved in thermotolerance.…”

Section: Discussionmentioning

confidence: 99%

Genome-wide expression profiling in leaves and roots of date palm (Phoenix dactylifera L.) exposed to salinity

et al. 2017

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BackgroundDate palm, as one of the most important fruit crops in North African and West Asian countries including Oman, is facing serious growth problems due to salinity, arising from persistent use of saline water for irrigation. Although date palm is a relatively salt-tolerant plant species, its adaptive mechanisms to salt stress are largely unknown.ResultsIn order to get an insight into molecular mechanisms of salt tolerance, RNA was profiled in leaves and roots of date palm seedlings subjected to NaCl for 10 days. Under salt stress, photosynthetic parameters were differentially affected; all gas exchange parameters were decreased but the quantum yield of PSII was unaffected while non-photochemical quenching was increased. Analyses of gene expression profiles revealed 2630 and 4687 genes were differentially expressed in leaves and roots, respectively, under salt stress. Of these, 194 genes were identified as commonly responding in both the tissue sources. Gene ontology (GO) analysis in leaves revealed enrichment of transcripts involved in metabolic pathways including photosynthesis, sucrose and starch metabolism, and oxidative phosphorylation, while in roots genes involved in membrane transport, phenylpropanoid biosynthesis, purine, thiamine, and tryptophan metabolism, and casparian strip development were enriched. Differentially expressed genes (DEGs) common to both tissues included the auxin responsive gene, GH3, a putative potassium transporter 8 and vacuolar membrane proton pump.ConclusionsLeaf and root tissues respond differentially to salinity stress and this study has revealed genes and pathways that are associated with responses to elevated NaCl levels and thus may play important roles in salt tolerance providing a foundation for functional characterization of salt stress-responsive genes in the date palm.Electronic supplementary materialThe online version of this article (doi:10.1186/s12864-017-3633-6) contains supplementary material, which is available to authorized users.

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“…The enhanced expression of HSP70 was reported to assist in translocation, proteolysis, translation, folding, aggregation, and refolding of denatured proteins (Gorantla et al, 2007; Zhang et al, 2010), while a methionine-rich chloroplast HSP has been shown to protect the thermolabile photosystem II and, consequently the whole-chain electron transport during heat stress (Peet et al, 2002). HSP101 for example, appears not to be required for growth in normal conditions, but plays a major role in tolerance to severe heat stresses and protein oxidative protection in Arabidopsis (Queitsch et al, 2000).…”

Section: Physiological and Molecular Indicators Of Tolerance To Incrementioning

confidence: 99%

Plant tolerance to high temperature in a changing environment: scientific fundamentals and production of heat stress-tolerant crops

Bita¹,

Geräts²

2013

Front. Plant Sci.

1,293

924

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Global warming is predicted to have a general negative effect on plant growth due to the damaging effect of high temperatures on plant development. The increasing threat of climatological extremes including very high temperatures might lead to catastrophic loss of crop productivity and result in wide spread famine. In this review, we assess the impact of global climate change on the agricultural crop production. There is a differential effect of climate change both in terms of geographic location and the crops that will likely show the most extreme reductions in yield as a result of expected extreme fluctuations in temperature and global warming in general. High temperature stress has a wide range of effects on plants in terms of physiology, biochemistry and gene regulation pathways. However, strategies exist to crop improvement for heat stress tolerance. In this review, we present recent advances of research on all these levels of investigation and focus on potential leads that may help to understand more fully the mechanisms that make plants tolerant or susceptible to heat stress. Finally, we review possible procedures and methods which could lead to the generation of new varieties with sustainable yield production, in a world likely to be challenged both by increasing population, higher average temperatures and larger temperature fluctuations.

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The role of arabidopsis AtFes1A in cytosolic Hsp70 stability and abiotic stress tolerance

Cited by 70 publications

References 38 publications

Protein Abundance Changes and Ubiquitylation Targets Identified after Inhibition of the Proteasome with Syringolin A

Protein Abundance Changes and Ubiquitylation Targets Identified after Inhibition of the Proteasome with Syringolin A

Genome-wide expression profiling in leaves and roots of date palm (Phoenix dactylifera L.) exposed to salinity

Plant tolerance to high temperature in a changing environment: scientific fundamentals and production of heat stress-tolerant crops

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