The role of apoptosis in cisplatin-induced ototoxicity in rats

Freitas, Marcos Rabelo de; Figueiredo, Aline Almeida; Brito, Gerly Anne de Castro; Leitão, Renata Ferreira de Carvalho; Junior, Jose Valdir de Carvalho; Junior, Raimundo Martins Gomes; Ribeiro, Ronaldo de Albuquerque

doi:10.1590/s1808-86942009000500022

Cited by 8 publications

(9 citation statements)

References 31 publications

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“…A similar result was observed by Rebert et al 28 , as well as by Freitas 23 . However, in hamsters, Church et al 29 reported prolongation of the I-IV interval with the 15 mg/kg dose of CDDP, suggesting retrocochlear damage by this drug.…”

Section: ■ Discussionsupporting

confidence: 87%

“…Tanaka et al 22 found a similar result as described with a 23% weight loss in animals treated with a dose of 13 mg/ kg. However, other authors such as Freitas et al 23 found a mean weight loss, after 3 days of evaluation, of 14% for the dose of 16 mg/kg of CDDP. In group C, there was an increase in the weight of the animals of approximately 6%, and although the animals were also submitted to the stress of intraperitoneal injection, they did not present diarrhea or reduced appetite like the animals exposed to CDDP.…”

Section: ■ Discussionmentioning

confidence: 87%

“…Cochlear dissection and optical microscopy were performed according to the method described by Freitas 23 . After preparation of the slides, the presence/absence of lesions in the stria vascularis and in the organ of Corti was evaluated and visualized with 400 x magnification in the Leica DM LS 2 optical microscope.…”

Section: ■ Methodsmentioning

confidence: 99%

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Study of the protective effect of dexamethasone on cisplatin-induced ototoxicity in rats

Capelo

Batista²,

Brito³

et al. 2017

Acta Cir. Bras.

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Study of the protective effect of dexamethasone on cisplatininduced ototoxicity in rats 1 9-Experimental SurgeryActa Cir Bras. 2017;32(10):873-880 AbstractPurpose: To evaluate the ability of dexamethasone to protect against cisplatin (CDDP)-induced ototoxicity. Methods: Male Wistar rats were divided into the following three groups: 1) Control (C): 6 animals received intraperitoneal (IP) saline solution, 8 ml/kg/day for four days; 2) C + CDDP: 11 animals received 8 ml/kg/day of IP saline and, 90 min after saline administration, 8 mg/kg/ day of IP CDDP for four days; and 3) DEXA15 + CDDP: 11 animals received IP dexamethasone 15 mg/kg/day and, 90 min after dexamethasone administration, received 8 mg/kg/day of IP CDDP for four days.Results: It was found that dexamethasone did not protect against weight loss in CDDPexposed animals. The mortality rate was comparable with that previously reported in the literature. The auditory threshold of animals in the DEXA15 + CDDP group was not significantly altered after exposure to CDDP. The stria vascularis of animals in the DEXA15 + CDDP group was partially preserved after CDDP exposure. Conclusions: Dexamethasone at the dose of 15 mg/kg/day partially protected against CDDPinduced ototoxicity, based on functional evaluation by brainstem evoked response audiontry (BERA) and morphological evaluation by optical microscopy. However, dexamethasone did not protect against systemic toxicity. 873Study of the protective effect of dexamethasone on cisplatin-induced ototoxicity in rats Capelo IOJ et al. Acta Cir Bras. 2017;32(10):873-880 874 kept in cages with free access to food and water and natural sleep and wake cycles.The animals were divided into three groups:Group 1 (n = 6) (Control; C): rats were treated on four consecutive days with saline solution at 8 ml/kg/day (total of 32 ml/kg). The animals were evaluated by brainstem evoked response audiometry (BERA) one day before (D0) and four days after (D4) treatment initiation.Group 2 (n = 11) (C + CDDP): rats were treated on four consecutive days with saline solution at 8 ml/kg/day (total of 32 ml/kg) and with CDDP at 8 mg/kg (total of 32 mg/kg) 90 min after saline administration. The animals were evaluated by BERA on D0 and D4.Group 3 (n = 11) (DEXA15 + CDDP): rats were treated on four consecutive days with dexamethasone at 15 mg/kg/day (total of 60 mg/kg) and with CDDP at 8 mg/kg/day (total of 32 mg/kg) 90 min after dexamethasone administration. The animals were evaluated by BERA on D0 and D4.Wistar rats underwent deep anesthesia with 80 mg/kg ketamine combined with 10 mg/kg xylazine. A previous otoscopy was performed, and animals with external and middle ear alterations were excluded from the study. Those with normal otoscopy received auditory evaluation (BERA) just prior to drug administration (D0). In all groups, the medications were injected intraperitoneally. In groups 2 and 3, the chemotherapy injection was performed 90 minutes after the initial injection of dexamethasone or saline solution. On the subsequent three days...

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Section: ■ Discussionsupporting

confidence: 87%

Section: ■ Discussionmentioning

confidence: 87%

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Study of the protective effect of dexamethasone on cisplatin-induced ototoxicity in rats

Capelo

Batista²,

Brito³

et al. 2017

Acta Cir. Bras.

Self Cite

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show abstract

“…the control group) through intraperitoneal route as indicated in the literature. [14,21] At the same day each treatment group received curcumin, vitamin E, and their combinations in that order. Curcumin was administered through intraperitoneal route at a dose of 200 mg/kg for 5 days at the same time of the day.…”

Section: Methodsmentioning

confidence: 99%

“…This argument was found to be compliant with the results of the study. In connection with this assumption, Freitas et al [21] detected that application of cis- Gevrek and Erdemir. Investigation of the effects of curcumin, vitamin E and their combination in cisplatin-induced testicular apoptosis using immunohistochemical technique platin at a dose of 16 mg/kg induced apoptosis three days later, as was revealed in our study.…”

Section: Bcl-2 Baxmentioning

confidence: 97%