The role of anion channels in osmotically activated taurine release from embryonic skate (<i>Raja eglanteria</i>) heart

Goldstein, Léon; Davis-Amaral, Erin M.; Blum, Patricia; Luer, Carl A.

doi:10.1242/jeb.198.12.2635

Journal of Experimental Biology

1995

DOI: 10.1242/jeb.198.12.2635

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The role of anion channels in osmotically activated taurine release from embryonic skate (Raja eglanteria) heart

Léon Goldstein

Erin M. Davis-Amaral

Patricia Blum

et al.

Abstract: Taurine, a major osmolyte of vertebrate hearts, is released from the skate heart at increased rates during hypotonic stress. We tested the hypothesis that this taurine release is mediated by chloride channels activated by swelling. Two inhibitors of the channels, NPPB and DIDS, inhibited the volume-activated release of taurine from embryonic skate hearts. These results support the hypothesis that swelling-activated chloride channels mediate the release of cardiac taurine.

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Cited by 3 publications

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Organic osmolyte channels in cell volume regulation in vertebrates

Perlman

Goldstein

1999

J. Exp. Zool.

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Volume‐activated organic osmolyte channels are found in a variety of vertebrates and cell types and show both common and disparate features. Upon exposure to hypotonic conditions, organic compounds such as taurine are released through these channels, reducing the intracellular solute concentration and thereby restoring cell volume. Various structurally diverse membrane proteins have been proposed as the channel. Accumulating evidence suggests that some of these proteins may play a more significant role as regulators than as the channel itself. Intracellular ionic strength may also modulate the release of organic osmolytes through these channels. J. Exp. Zool. 283:725–733, 1999. © 1999 Wiley‐Liss, Inc.

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Organic osmolyte channels in cell volume regulation in vertebrates

Perlman

Goldstein

1999

J. Exp. Zool.

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Volume changes in cardiac ventricles from Aplysia brasiliana upon exposure to hyposmotic shock

Souza

Scemes

2000

Comparative Biochemistry and Physiology Part A: Molecular &

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Molecular distribution of volume-regulated chloride channels (ClC-2 and ClC-3) in cardiac tissues

Britton

Hatton

Rossow

et al. 2000

American Journal of Physiology-Heart and Circulatory Physiology

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The molecular identification of cardiac chloride channels has provided probes to investigate their distribution and abundance in heart. In this study, the molecular expression and distribution of volume-regulated chloride channels ClC-2 and ClC-3 in cardiac tissues were analyzed and quantified. Total RNA was isolated from atria and ventricles of several species (dog, guinea pig, and rat) and subjected to a quantitative RT-PCR strategy. ClC-2 and ClC-3 mRNA expression were calculated relative to beta-actin expression within these same tissues. The transcriptional levels of ClC-3 mRNA were between 1.8 and 10.2% of beta-actin expression in atria and between 3.4 and 8.6% of beta-actin in ventricles (n = 3 for each tissue). The levels of ClC-2 in both atria and ventricles were significantly less than those measured for ClC-3 (n = 3; P < 0.05). ClC-2 mRNA levels were between 0.04-0.08% and 0.03-0.18% of beta-actin expression in atria and ventricles, respectively (n = 3 for each tissue). Immunoblots of atrial and ventricular wall protein extracts demonstrated ClC-2- and ClC-3-specific immunoreactivity at 97 and 85 kDa, respectively. Immunohistochemical localization in guinea pig cardiac muscle demonstrates a ubiquitous distribution of ClC-2 and ClC-3 channels in the atrial and ventricular wall. Confocal analysis detected colocalization of ClC-2 and ClC-3 in sarcolemmal membranes and distinct ClC-3 immunoreactivity in cytoplasmic regions. The molecular expression of ClC-2 and ClC-3 in cardiac tissue is consistent with the proposed role of these chloride channels in the regulation of cardiac cell volume and the modulation of cardiac electrical activity.

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The role of anion channels in osmotically activated taurine release from embryonic skate (Raja eglanteria) heart

Cited by 3 publications

References 9 publications

Organic osmolyte channels in cell volume regulation in vertebrates

Organic osmolyte channels in cell volume regulation in vertebrates

Volume changes in cardiac ventricles from Aplysia brasiliana upon exposure to hyposmotic shock

Molecular distribution of volume-regulated chloride channels (ClC-2 and ClC-3) in cardiac tissues

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