The role of alkylhydroxybenzenes in the adaptation of Micrococcus luteus to heat shock

Stepanenko, I. Yu.; Mulyukin, A. L.; Kozlova, A. N.; Nikolaev, Yu. A.; Эль-Регистан, Г. И.

doi:10.1007/s11021-005-0023-x

Cited by 3 publications

(6 citation statements)

References 5 publications

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“…3), as well as the heat shock protein induction value F i derived from them (Table 4) in the cells of the E. coli ibpA'::luxCDABE test strain sub jected to heat shock after preincubation (60 min) with ARs. After heat treatment, a statistically important, albeit minor (11-17% compared to the control) increase in viable cell numbers (determined as CFU) was observed only in the samples pretreated with C 7 and C 9 AR (10 -6 -10 -4 M), which was in good agree ment with data on the heat protective properties of short chain AR homologues presented above and known from the literature [21,22]. At the same time, the number of cells retaining viability decreased pro gressively in the order C 11 AR → C 18 AR, and for each of the long chain homologues it decreased with an increase of their acting concentrations (Fig.…”

Section: Effect Of Alkylhydroxybenzenes On Luciferase Ther Mal Stabilsupporting

confidence: 90%

“…For example, in the samples preincubated with C 12 and C 18 (Table 4). Such an effect of C 7 and C 9 AR may be ascribed to their adaptogenic and protective functions demonstrated previously [10,[21][22][23]. HSP synthesis induction factor F 2i and the residual number of CFU after heat shock of the cells pretreated with ARs were compared with the same values for non heated cells, taken as 100%, at each AR concentration; this revealed the adaptogenic function of long chain ARs (Table 4).…”

Section: Effect Of Alkylhydroxybenzenes On Luciferase Ther Mal Stabilmentioning

confidence: 54%

“…Studies of AR functions in generation of bacterial stress response demonstrated that various extreme factors (for example, heat shock) stimulated AR biosynthesis [21], while elevated AR levels affected cell stability [8,22,23] by means of increased func tional and operational stability of the enzymes [9,20,24] on the one hand, and activation of stress gene expression, on the other [10,13]. It was therefore log ical to assume that changes in AR concentration in a microbial culture can affect the induction of heat shock protein synthesis, in particular, syntesis of the IbpA chaperone [6].…”

Section: Effect Of Alkylhydroxybenzenes On Luciferase Ther Mal Stabilmentioning

confidence: 99%

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Effect of alkylresorcinols on thermal denaturation and refolding of bacterial luciferase and synthesis of heat shock proteins revealed in the luminescent molecular and cellular test systems

et al. 2014

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Molecular and cellular luminescent biotests were used to reveal the effects of five alkylresorcinol homologues (C 7 , C 9 , C 11 , C 12 , and C 18 AR) on the thermally induced denaturation and refolding of bac terial luciferases, as well as on the synthesis of heat shock proteins. The ARs activities were found to depend on their fine structure and concentration. The direct heat protective effect of short chain C 7 and C 9 AR on the chromatographically pure Photobactrium leiognathii luciferase/oxidoreductase was shown within a broad range of concentrations (10 -6 -10 -3 M). The long chain ARs homologues exhibited a similar heat protective effect at micromolar concentrations only, while their millimolar concentrations increased the sensitivity of the model proteins to thermal treatment. The recombinant strain Escherichia coli K12 MG1655, bearing con stitutively expressed Vibrio fischeri luxAB genes was used to investigate the ARs effect on the intracellular chaperone independent refolding of bacterial luciferase. The functional activity of heat inactivated enzyme was restored by micromolar concentrations of short chain ARs, while long chain homologues inhibited refolding over the wide concentration range. The recombinant luminescent E. coli strain bearing the induc ible ibpA'::luxCDABE genetic construction was used to determine the effect of ARs on the synthesis of heat shock proteins (HSP). The preincubation mode of bacterial cells with long chain alkylresorcinols led to the dose dependent stimulation of HSP synthesis (2.7 to 4 times), which confirmed that ARs function as "alar mones." Subsequent thermal treatment resulted in a 5 to 15 fold decrease of the following HSP induction compared to the control, while the number of viable cells opposite increased by 1.5 to 4 fold. Thus, pretreat ment of the bacterial cells with long chain ARs resulted in their preadaptation to subsequent thermally induced stress. Short chain ARs caused less pronounced HSP suppression, although this was still was accom panied by increased heat resistance of the AR pretreated bacterial cells.

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Section: Effect Of Alkylhydroxybenzenes On Luciferase Ther Mal Stabilsupporting

confidence: 90%

Section: Effect Of Alkylhydroxybenzenes On Luciferase Ther Mal Stabilmentioning

confidence: 54%

Section: Effect Of Alkylhydroxybenzenes On Luciferase Ther Mal Stabilmentioning

confidence: 99%

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Effect of alkylresorcinols on thermal denaturation and refolding of bacterial luciferase and synthesis of heat shock proteins revealed in the luminescent molecular and cellular test systems

et al. 2014

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“…This was earlier researched in sev eral in vitro [7][8][9] and in vivo [3][4][5][6] models and inves tigated in this work using the recombinant strain E. coli recA'::lux. A distinctive feature of this genetic system that facilitates attaining our goal is its capacity to respond to UV irradiation caused DNA impairments with bioluminescence.…”

Section: Discussionmentioning

confidence: 99%

“…Another aspect of the biological activity of AHBs is their functioning as adaptogens involved in developing of microbial resistance to a wide variety of deleterious factors [3]. In a number of studies, the protective effects of AHBs on the cells of bacterial and yeast cul tures under various kinds of stress [4,5], including UV irradiation [6], were demonstrated. This provided the foundation for more detailed research on the mecha nisms of action of the above group of microbial auto regulators.…”

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confidence: 99%

Involvement of alkylhydroxybenzenes in the Escherichia coli response to the lethal effect of UV irradiation

et al. 2012

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This work is concerned with the role of alkylhydroxybenzenes (AHBs), chemical analogs of the autoregulatory microbial d 1 factors, on the development of the stress response of bacterial cells to UV irradi ation, including SOS system induction, preservation of cell viability, and S R phase transitions of the Escherichia coli test strain with the bioluminescence genes cloned under the control of the recA gene pro moter. UV irradiation, a natural stress factor, and an increase in AHB concentrations were found to elicit uni form responses in bacteria, indicating that AHBs function as alarmones, i.e., alarm signals. It was revealed that preincubating bacteria with alkylhydroxybenzenes considerably enhanced their viability upon irradiation with lethal UV doses; this was accompanied by a relative decrease in the SOS response activity and a concom itant increase in the frequency of phase transitions. The efficiency of the protective action of AHBs increased with an increase in their hydrophobicity degree. The probable mechanism of the protective effect of AHBs is discussed, based on their capacity for the interaction with biopolymers, which results in changing their struc tural organization and conferring resistance to a broad spectrum of stress factors. Such a "passive" protective mechanism reduces the susceptibility of DNA to UV irradiation, causing a decrease in the parameters related to the SOS system induction that is responsible for the "active" protective mechanism in bacterial cells. As a result, viability retention under the lethal influence of UV irradiation is possible at minimal values of repair activity and is accompanied by an increase in the phenotypic variability of the surviving part of a bacterial population.

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Bacterial metabolites from intra- and inter-species influencing thermotolerance: the case of Bacillus cereus and Geobacillus stearothermophilus

2016

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Bacterial metabolites with communicative functions could provide protection against stress conditions to members of the same species. Yet, information remains limited about protection provided by metabolites in Bacillus cereus and inter-species. This study investigated the effect of extracellular compounds derived from heat shocked (HS) and non-HS cultures of B. cereus and Geobacillus stearothermophilus on the thermotolerance of non-HS vegetative and sporulating B. cereus. Cultures of B. cereus and G. stearothermophilus were subjected to HS (42 or 65 °C respectively for 30 min) or non-HS treatments. Cells and supernatants were separated, mixed in a combined array, and then exposed to 50 °C for 60 min and viable cells determined. For spores, D values (85 and 95 °C) were evaluated after 120 h. In most cases, supernatants from HS B. cereus cultures added to non-HS B. cereus cells caused their thermotolerance to increase (D 12.2-51.9) when compared to supernatants from non-HS cultures (D 7.4-21.7). While the addition of supernatants from HS and non-HS G. stearothermophilus cultures caused the thermotolerance of non-HS cells from B. cereus to decrease initially (D 3.7-7.1), a subsequent increase was detected in most cases (D 18-97.7). In most cases, supernatants from sporulating G. stearothermophilus added to sporulating cells of B. cereus caused the thermotolerance of B. cereus 4810 spores to decline, whereas that of B. cereus 14579 increased. This study clearly shows that metabolites in supernatants from either the same or different species (such as G. stearothermophilus) influence the thermotolerance of B. cereus.

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The role of alkylhydroxybenzenes in the adaptation of Micrococcus luteus to heat shock

Cited by 3 publications

References 5 publications

Effect of alkylresorcinols on thermal denaturation and refolding of bacterial luciferase and synthesis of heat shock proteins revealed in the luminescent molecular and cellular test systems

Effect of alkylresorcinols on thermal denaturation and refolding of bacterial luciferase and synthesis of heat shock proteins revealed in the luminescent molecular and cellular test systems

Involvement of alkylhydroxybenzenes in the Escherichia coli response to the lethal effect of UV irradiation

Bacterial metabolites from intra- and inter-species influencing thermotolerance: the case of Bacillus cereus and Geobacillus stearothermophilus

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