The Role of a Minimum Immunohistochemical Antibody Panel in Confirming Undifferentiated Nasopharyngeal Carcinoma: A Cross-Sectional Study at the Muhimbili National Hospital, Dar-es-Salaam, Tanzania

Mremi, Alex; Yahaya, James J.; Abraham, Zephania Saitabau; Mwakigonja, Amos

doi:10.4103/nmj.nmj_134_19

Cited by 2 publications

(3 citation statements)

References 18 publications

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“…This was done as previously described. 19 Briefly, review of the diagnoses was done by the first author (V.E.S.) and a qualified, senior anatomical pathologist (A.R.M.)…”

Section: Methodsmentioning

confidence: 99%

“…using an Olympus (CX31RBSF Model) light microscope equipped with a digital camera (Olympus Corporation, Tokyo, Japan) as previously described. 19 , 20…”

Section: Methodsmentioning

confidence: 99%

“…A positive control included a tissue known to have EBV infection, whereas for negative controls the test antibody was omitted and replaced by PBS. 19 …”

Section: Methodsmentioning

confidence: 99%

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Epstein-Barr Virus Latent Membrane Protein-1 Expression in Nasopharyngeal Carcinoma

Salano

Mwakigonja

Abdulshakoor

et al. 2021

JCO Global Oncology

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PURPOSE Nasopharyngeal carcinoma (NPC), a malignant neoplasm of the epithelium covering the nasopharynx, is a rare disease in most parts of the world. Epstein-Barr virus (EBV), the most potent oncogenic virus, coupled with environmental and genetic factors has been identified to play a role in the development of NPC. An array of methods for detecting the virus do exist, from serologic detection of antibodies to DNA amplification. There is paucity of local data on the status of EBV infection in relation to NPC within the region, and this study attempts to shed more light on the subject. METHODS This was a retrospective cross-sectional laboratory-based study on histologically confirmed, archived tissues from July 2015 to June 2019. Immunohistochemistry expression of latent membrane protein-1 (LMP-1) was used to detect EBV infection in the tissues. RESULTS A total of 71 cases were enrolled in this study. The mean age was 47.87 years ± 16.84 years with a male-to-female ratio of 1.5:1. There was a unimodal distribution of EBV detection, with the peak (26.8%) at 36-45 years. About 45.1% of the 71 samples tested positive for LMP-1, all of which were nonkeratinizing carcinoma. Nonkeratinizing carcinoma was the most common histopathologic subtype (n = 67; 94.4%), with the majority (38 of 67; 56.7%) being undifferentiated and 29 of 67 (43.3%) differentiated. Keratinizing and basaloid subtypes had two cases each, representing 2.8%. CONCLUSION A significant proportion of NPC, particularly nonkeratinizing histologic subtype, seems to show LMP-1 positivity by immunohistochemistry, which may be adopted in resource-constrained settings to detect EBV infection in these tissue biopsies.

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“…This was done as previously described. 19 Briefly, review of the diagnoses was done by the first author (V.E.S.) and a qualified, senior anatomical pathologist (A.R.M.)…”

Section: Methodsmentioning

confidence: 99%

“…using an Olympus (CX31RBSF Model) light microscope equipped with a digital camera (Olympus Corporation, Tokyo, Japan) as previously described. 19 , 20…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Epstein-Barr Virus Latent Membrane Protein-1 Expression in Nasopharyngeal Carcinoma

Salano

Mwakigonja

Abdulshakoor

et al. 2021

JCO Global Oncology

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Immunohistochemical expression of P53 protein in nephroblastoma: a predictor of unfavorable prognosis

Morgan,

Yahaya,

Ngaiza

et al. 2023

J Egypt Natl Canc Inst

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Objective Immunohistochemical expression of P53 protein is so closely related to status of mutation of P53 gene which is tightly linked with pathogenesis of nephroblastoma or Wilms tumor. This study aims to determine the immunohistochemical expression of P53 protein and its predictors in formalin-fixed paraffin-embedded tissue blocks of patients with nephroblastoma. Materials and methods A series of 83 histologically diagnosed cases of nephroblastoma from formalin-fixed paraffin-embedded tissue blocks archived at the Department of Pathology, Makerere University, in Kampala, Uganda, were analyzed. Monoclonal anti-p53 antibody (DO-7, DAKO) was used to assess the expression of P53 protein expression. Multivariable logistic regression analysis was performed to determine the predictors of P53 protein immunohistochemical expression, and statistical significance was considered when p-value was less than 0.05. Results Most (42.2%, n = 35) of the cases were in advanced tumor stages (III–V), and almost one-quarter (21.7%, n = 18) of the cases were in high-risk group. The immunohistochemical expression of P53 protein was (8.4%, n = 7), and there were more (83.3%, n = 5) positive anaplastic cases for P53 protein compared with (2.6%, n = 2) of P53 expression for non-anaplastic cases. High risk (AOR = 3.42, 95% CI = 7.91–12.55, p = 0.037) and anaplasia (AOR = 1.41, 95% CI = 13.85–4.46, p = 0.001) were potential predictors of immunohistochemical expression of P53 protein. Conclusion Most of patients with nephroblastoma in resources-limited settings are diagnosed with advanced clinical stages. Association of P53 protein with anaplasia found in this study indicates the possibility of having novel target therapy for treatment of patients with anaplastic form of nephroblastoma with a focus of identifying molecules that lead to its suppression in such subpopulations of patients with nephroblastoma.

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The Role of a Minimum Immunohistochemical Antibody Panel in Confirming Undifferentiated Nasopharyngeal Carcinoma: A Cross-Sectional Study at the Muhimbili National Hospital, Dar-es-Salaam, Tanzania

Cited by 2 publications

References 18 publications

Epstein-Barr Virus Latent Membrane Protein-1 Expression in Nasopharyngeal Carcinoma

Epstein-Barr Virus Latent Membrane Protein-1 Expression in Nasopharyngeal Carcinoma

Immunohistochemical expression of P53 protein in nephroblastoma: a predictor of unfavorable prognosis

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