The role of a conserved dodecamer sequence in yeast mitochondrial gene expression

Butow, Ronald A.; Hong, Zhu; Perlman, Philip S.; Conrad-Webb, Heather

doi:10.1139/g89-134

Cited by 41 publications

(35 citation statements)

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“…Therefore, we speculated that all these gene products may function as PAPs in the mitochondria of the organisms listed above. We could not find regulatory cytoplasmic PAP homologs with an mt targeting signal peptide in yeast, which is consistent with the report that yeast mt RNAs lack a poly(A) tail (11).…”

Section: Retrieval Of a Candidate Gene Encoding Human Mt Pap Insupporting

confidence: 92%

“…In plant mitochondria, polyadenylation promotes mRNA degradation as in bacteria and chloroplasts (10). In yeast mitochondria, mRNAs lack the poly(A) tail, and mt gene expression seems to be regulated by the mtDNA-encoded conserved dodecamer sequence at its 3Ј terminus (11). It is known that the stability of yeast mitochondrial (mt) mRNA is controlled by mRNA-binding proteins (12)(13)(14).…”

Section: Mammalian Mitochondrial (Mt) Mrnas Have Short Poly(a) Tails mentioning

confidence: 99%

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Human Mitochondrial mRNAs Are Stabilized with Polyadenylation Regulated by Mitochondria-specific Poly(A) Polymerase and Polynucleotide Phosphorylase

Nagaike

Suzuki

Katoh

et al. 2005

Journal of Biological Chemistry

169

197

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Mammalian mitochondrial (mt) mRNAs have short poly(A) tails at their 3 termini that are post-transcriptionally synthesized by mt poly(A) polymerase (PAP). The polyadenylation of mt mRNAs is known to be a key process needed to create UAA stop codons that are not encoded in mtDNA. In some cases, polyadenylation is required for the tRNA maturation by editing of its 3 terminus. However, little is known about the functional roles the poly(A) tail of mt mRNAs plays in mt translation and RNA turnover. Here we show human mt PAP (hmtPAP) and human polynucleotide phosphorylase (hPNPase) control poly(A) synthesis in human mitochondria. Partial inactivation of hmtPAP by RNA interference using small interfering RNA in HeLa cells resulted in shortened poly(A) tails and decreased steady state levels of some mt mRNAs as well as their translational products. Moreover, knocking down hmtPAP generated markedly defective mt membrane potentials and reduced oxygen consumption. In contrast, knocking down hPNPase showed significantly extended poly(A) tails of mt mRNAs. These results demonstrate that the poly(A) length of human mt mRNAs is controlled by polyadenylation by hmtPAP and deadenylation by hPNPase, and polyadenylation is required for the stability of mt mRNAs.

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Section: Retrieval Of a Candidate Gene Encoding Human Mt Pap Insupporting

confidence: 92%

Section: Mammalian Mitochondrial (Mt) Mrnas Have Short Poly(a) Tails mentioning

confidence: 99%

Human Mitochondrial mRNAs Are Stabilized with Polyadenylation Regulated by Mitochondria-specific Poly(A) Polymerase and Polynucleotide Phosphorylase

Nagaike

Suzuki

Katoh

et al. 2005

Journal of Biological Chemistry

169

197

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“…SUV3 was originally identified in Saccharomyces cerevisiae as a dominant suppressor allele, SUV3-1, which suppressed a 3Ј dodecamer deletion phenotypes on the VAR1 gene (1)(2)(3). Later characterization showed that the SUV3-1 allele carries a missense mutation, Val-272 to Leu (named V272L in this study) (4).…”

mentioning

confidence: 76%

Uncoupling the Roles of the SUV3 Helicase in Maintenance of Mitochondrial Genome Stability and RNA Degradation

Guo¹,

Chen²,

Wang³

et al. 2011

Journal of Biological Chemistry

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Background: SUV3 helicase is an essential component of mitochondrial degradosome. Results: Using specific genetic mutants, SUV3 was demonstrated to participate in mtDNA replication, which requires ATPase activity and the C-terminal conserved region. Conclusion: SUV3 plays two distinctive roles in maintaining mtDNA stability and RNA turnover. Significance: This study suggests a direct role of SUV3 in maintaining mtDNA stability, paving a foundation for biochemical investigation.

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“…Yeast mitochondrial mRNAs lack poly(A) tails. Instead, an encoded dodecamer sequence regulates RNA stability in this system (10). In human mitochondria, it appears that polyadenylation positively or negatively regulates steady-state levels of mitochondrial mRNAs in a transcript-specific manner (11)(12)(13).…”

Section: Introductionmentioning

confidence: 99%

Targeted depletion of a mitochondrial nucleotidyltransferase suggests the presence of multiple enzymes that polymerize mRNA 3′ tails in Trypanosoma brucei mitochondria

Kao

Read

2007

Molecular and Biochemical Parasitology

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Polyadenylation plays an important role in regulating RNA stability in Trypanosoma brucei mitochondria. To date, little is known about the enzymes responsible for the addition of mRNA 3′ tails in this system. In this study, we characterize a trypanosome homolog of the human mitochondrial poly(A) polymerase, which we term kPAP2. kPAP2 is mitochondrially localized and expressed in both bloodstream and procyclic form trypanosomes. Targeted gene depletion using RNAi showed that kPAP2 is not required for T. brucei growth in either bloodstream or procyclic life stages, nor is it essential for differentiation from bloodstream to procyclic form. We also demonstrate that steady state abundance of several mitochondrial RNAs was largely unaffected upon kPAP2 downregulation. Interestingly, mRNA 3′ tail analysis of several mRNAs from both life cycle stages in uninduced kPAP2 RNAi cells demonstrated that tail length and uridine content are both regulated in a transcript-specific manner. mRNA-specific tail lengths were maintained upon kPAP2 depletion. However, the percentage of uridine residues in 3′ tails was increased, and conversely the percentage of adenosine residues was decreased, in a distinct subset of mRNAs when kPAP2 levels were downregulated. Thus, kPAP2 apparently contributes to the incorporation of adenosine residues in 3′ tails of some, but not all, mitochondrial mRNAs. Together, these data suggest that multiple nucleotidyltransferases act on mitochondrial mRNA 3′ ends, and that these enzymes are somewhat redundant and subject to complex regulation.

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The role of a conserved dodecamer sequence in yeast mitochondrial gene expression

Cited by 41 publications

References 0 publications

Human Mitochondrial mRNAs Are Stabilized with Polyadenylation Regulated by Mitochondria-specific Poly(A) Polymerase and Polynucleotide Phosphorylase

Human Mitochondrial mRNAs Are Stabilized with Polyadenylation Regulated by Mitochondria-specific Poly(A) Polymerase and Polynucleotide Phosphorylase

Uncoupling the Roles of the SUV3 Helicase in Maintenance of Mitochondrial Genome Stability and RNA Degradation

Targeted depletion of a mitochondrial nucleotidyltransferase suggests the presence of multiple enzymes that polymerize mRNA 3′ tails in Trypanosoma brucei mitochondria

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