The Role of 1‐Aminocyclopropane‐1‐Carboxylate (ACC) Deaminase Enzyme in Leguminous Nodule Senescence

Tittabutr, Panlada; Boonkerd, Nantakorn; Teaumroong, Neung

doi:10.1002/9781119053095.ch71

Cited by 1 publication

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“…Changes in the ratio of sugar/nitrogen in nodules is a signal for the activation of abscisic acid, which often occurs in plants. The rate of this change was supported by lower carbon and higher nitrogen metabolite availabilities in nodules (Tittabutr et al, 2015a). Moreover, this change may induce nodule senescence, which resulted in reduced nitrogen fixation in mung bean plants inoculated with SUTN9-2:pMG103::acdRS at 19 DAI under normal conditions.…”

Section: Discussionmentioning

confidence: 99%

“…Moreover, this change may induce nodule senescence, which resulted in reduced nitrogen fixation in mung bean plants inoculated with SUTN9-2:pMG103::acdRS at 19 DAI under normal conditions. Nodule senescence caused by environmental stress generally occurs faster than developmental senescence because ethylene, a senescence hormone, was found to be strongly induced under environmental stress conditions (Puppo et al, 2005;Tittabutr et al, 2015a). Previous studies found that water deficit conditions reduced the supply of carbon (malate).…”

Section: Discussionmentioning

confidence: 99%

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Effects of Increased 1-Aminocyclopropane-1-Carboxylate (ACC) Deaminase Activity in <i>Bradyrhizobium</i> sp. SUTN9-2 on Mung Bean Symbiosis under Water Deficit Conditions

et al. 2020

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Bacteria exhibiting 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase activity, which inhibits the biosynthesis of ethylene in higher plants, promote plant growth through the degradation of ethylene precursors, such as ACC. ACC deaminase activity in Bradyrhizobium sp. SUTN9-2 was enhanced by genetic engineering and adaptive laboratory evolution (ALE)-based methods. The transferal of a plasmid containing the acdR and acdS genes into SUTN9-2 was genetic engineering improved, while the ALE method was performed based on the accumulation of an adaptive bacterial population that continuously grew under specified growth conditions for a long time. ACC deaminase enzyme activity was 8.9-fold higher in SUTN9-2:pMG103::acdRS and 1.4-fold higher in SUTN9-2 (ACCDadap) than in the wild-type strain. The effects of increased activity were examined in the host plant (Vigna radiata (L.) R.Wilczek SUT1). The improved strains enhanced nodulation in early stage of plant growth. SUTN9-2:pMG103::acdRS also maintained nitrogen fixation under water deficit conditions and increased the plant biomass after rehydration. Changes in nucleotides and amino acids in the AcdS protein of SUTN9-2 (ACCDadap) were then investigated. Some nucleotides predicted to be located in the ACC-binding site were mutated. These mutations may have increased ACC deaminase activity, which enhanced both symbiotic interactions and drought tolerance and promoted recovery after rehydration more than lower ACC deaminase activity. Adaptive evolution represents a promising strategy for further applications in the field.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%