The Role, Function, and Mechanism of Long Intergenic Noncoding RNA1184 (linc01184) in Colorectal Cancer

Abstract

Background. Long intergenic noncoding RNA1184 (linc01184) has been recently discovered; however, its role in human diseases is limited to date. The present study is aimed at investigating the expression pattern and mechanism of linc01184 in colorectal cancer (CRC) tumorigenesis. Methods. The expression of linc01184 in CRC tissues and cell lines was compared with that in normal controls. The functions of linc01184 in CRC cells were identified by overexpression and small interfering RNA (siRNA) approaches in vitro. Meanwhile, the target gene prediction software, luciferase reporter, RNA pull-down, and western blotting assays were used to analyze the oncogenic mechanism. Results. We found that linc01184 was obviously upregulated in CRC tissues and cells when compared to normal controls, and its upregulation had a positive association with the CRC progression. linc01184 knockdown significantly suppressed CRC cell proliferation and invasion and promoted apoptosis. Besides, linc01184 acted as a competitive endogenous RNA (ceRNA) by directly binding to microRNA-331 (miR-331), and its overexpression resulted in notable increases of human epidermal growth factor receptor 2 (HER2), phosphorylated Ser/Thr kinases (p-Akt), and extracellular regulated protein kinase 1/2 (p-ERK1/2) at posttranscriptional levels in CRC cells, which were antagonized by miR-331. Conclusions. The findings reveal for the first time that linc01184 is an enhancer for the proliferation and invasion of CRC by functioning as a ceRNA through the linc01184-miR-331-HER2-p-Akt/ERK1/2 pathway regulatory network.

Construction of a ceRNA Network and Comprehensive Analysis of lncRNA in Hepatocellular Carcinoma

“…Silencing LINC01006 suppresses the viability of HCC cells in vitro and suppresses tumor growth in vivo [33]. The roles of LINC00847 and LINC01184 have not been reported in HCC, but several studies have demonstrated that they have a carcinogenic effect in many other cancers [34][35][36]. Thus, a novel mRNA-miRNA-lncRNA network has been established to be associated with the hepatocellular carcinoma prognosis.…”

A novel immune-related eRNAs prognostic signature to predict the immunotherapy response for thyroid carcinoma

“…Sui et al demonstrated that LINC01184 was highly expressed in colorectal cancer, and it could affect the the proliferation and invasion of colorectal cancer cells through the linc01184-miR-331-HER2-p-Akt/ERK1/2 pathway 49 . Subsequently, the risk score was calculated and separated all patients into highand low-risk subgroups based on median risk score, and Kaplan-Meier survival curves indicated that the high-risk groups had poorer clinical results than that of the low-risk groups.…”

Construction and Validation of an Immune-Related lncRNA Prognosis Model for Thyroid Cancer

“…LINC01614 is associated with clinical stage in patients with glioma and NSCLC [15,25]. LINC01184 is associated with the several clinicopathological profiles of rectal cancer, including clinical stage, tumor size, lymph nodes, and distal metastasis [26]. Regarding LINC00900, Wang et al showed that the expression of LINC00900 increased with tumor grade in patients with primary glioblastoma [27].…”