The role and therapeutic potential of stem cells in skeletal muscle in sarcopenia

Cai, Zijun; Liu, Di; Yang, Yuntao; Xie, Wenqing; He, Miao; Dong-sheng, YU; Wu, Yuxiang; Wang, Xiuhua; Xiao, Wenfeng; Li, Yusheng

doi:10.1186/s13287-022-02706-5

Cited by 22 publications

(10 citation statements)

References 120 publications

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“…Muscle tissue is a good example of highly aligned tissue, with fiber alignment playing a crucial role in dictating its mechanical function (muscle contraction). Tissue-engineered muscle constructs that are able to mimic the native microarchitecture can be used as functional in vitro models, − and they are seen as a promising treatment for muscle traumas or disorders such as volumetric muscle loss (VML) , and sarcopenia. , In this study, mouse myoblasts (C2C12) were used as a proof-of-concept model to test the method’s ability to generate aligned skeletal muscle tissues. C2C12 cells were found to be highly viable (Figure B) and homogeneously distributed upon sizing at day 0 (Figure A).…”

Section: Resultsmentioning

confidence: 99%

Macroporous Aligned Hydrogel Microstrands for 3D Cell Guidance

Rizzo

Bonato

Chansoria

et al. 2022

ACS Biomater. Sci. Eng.

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Tissue engineering strongly relies on the use of hydrogels as highly hydrated 3D matrices to support the maturation of laden cells. However, because of the lack of microarchitecture and sufficient porosity, common hydrogel systems do not provide physical cell-instructive guidance cues and efficient transport of nutrients and oxygen to the inner part of the construct. A controlled, organized cellular alignment and resulting alignment of secreted ECM are hallmarks of muscle, tendons, and nerves and play an important role in determining their functional properties. Although several strategies to induce cellular alignment have been investigated in 2D systems, the generation of cell-instructive 3D hydrogels remains a challenge. Here, we report on the development of a simple and scalable method to efficiently generate highly macroporous constructs featuring aligned guidance cues. A precross-linked bulk hydrogel is pressed through a grid with variable opening sizes, thus deconstructing it into an array of aligned, high aspect ratio microgels (microstrands) with tunable diameter that are eventually stabilized by a second photoclick cross-linking step. This method has been investigated and optimized both in silico and in vitro, thereby leading to conditions with excellent viability and organized cellular alignment. Finally, as proof of concept, the method has been shown to direct aligned muscle tissue maturation. These findings demonstrate the 3D physical guidance potential of our system, which can be used for a variety of anisotropic tissues and applications.

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Section: Resultsmentioning

confidence: 99%

Macroporous Aligned Hydrogel Microstrands for 3D Cell Guidance

Rizzo

Bonato

Chansoria

et al. 2022

ACS Biomater. Sci. Eng.

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“…The prevalence of sarcopenia is related to skeletal muscle loss with aging [ 31 ]. Although the pathogenesis of sarcopenia remains unclear, sarcopenia is likely to be closely related to MuSC dysfunction [ 32 ]. MuSCs from 24-month-old mice exhibited a loss in migration speed, which is a key component of skeletal muscle regeneration [ 33 ].…”

Section: Discussionmentioning

confidence: 99%

Decrease in the expression of muscle-specific miRNAs, miR-133a and miR-1, in myoblasts with replicative senescence

2023

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Muscles that are injured or atrophied by aging undergo myogenic regeneration. Although myoblasts play a pivotal role in myogenic regeneration, their function is impaired with aging. MicroRNAs (miRNAs) are also involved in myogenic regeneration. MiRNA (miR)-1 and miR-133a are muscle-specific miRNAs that control the proliferation and differentiation of myoblasts. In this study, we determined whether miR-1 and miR-133a expression in myoblasts is altered with cellular senescence and involved in senescence-impaired myogenic differentiation. C2C12 murine skeletal myoblasts were converted to a replicative senescent state by culturing to a high passage number. Although miR-1 and miR-133a expression was largely induced during myogenic differentiation, expression was suppressed in cells at high passage numbers (passage 10 and/or passage 20). Although the senescent myoblasts exhibited a deterioration of myogenic differentiation, transfection of miR-1 or miR-133a into myoblasts ameliorated cell fusion. Treatment with the glutaminase 1 inhibitor, BPTES, removed senescent cells from C2C12 myoblasts with a high passage number, whereas myotube formation and miR-133a expression was increased. In addition, primary cultured myoblasts prepared from aged C57BL/6J male mice (20 months old) exhibited a decrease in miR-1 and miR-133a levels compared with younger mice (3 months old). The results suggest that replicative senescence suppresses muscle-specific miRNA expression in myoblasts, which contributes to the senescence-related dysfunction of myogenic regeneration.

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“…Unlike satellite cells, which can only undergo myogenic differentiation, Muscle-derived stem cells (MDSCs) are thought to differentiate into muscular, vascular, nerve, and bone lineage cells [ 82 ]. Adipogenesis of MDSCs may cause fat infiltration in skeletal muscle, leading to the loss of muscle strength and promoting the occurrence and development of sarcopenia [ 83 ].…”

Section: Discussionmentioning

confidence: 99%

Bibliometric Analysis of the Knowledge Base and Future Trends on Sarcopenia from 1999–2021

Xiao

Deng

Tan

et al. 2022

IJERPH

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Sarcopenia is characterized by progressive loss of muscle mass and function, and it is becoming a serious public health problem with the aging population. However, a comprehensive overview of the knowledge base and future trends is still lacking. The articles and reviews with “sarcopenia” in their title published from 1999 to 2021 in the SCIE database were retrieved. We used Microsoft Excel, VOSviewer, and CiteSpace to conduct a descriptive and bibliometric analysis. A total of 3582 publications were collected, from 4 published in 2000 increasing dramatically to 850 documents in 2021. The USA was the most productive country, with the most citations. The Catholic University of the Sacred Heart and Landi F were the most influential organization and author in this field, respectively. The core journal in this field was the Journal of Cachexia Sarcopenia and Muscle. According to the analysis of keywords and references, we roughly categorized the main research areas into four domains as follows: 1. Definition and diagnosis; 2. Epidemiology; 3. Etiology and pathogenesis; 4. Treatments. Comparing different diagnostic tools and the epidemiology of sarcopenia in different populations are recent hotspots, while more efforts are needed in the underlying mechanism and developing safe and effective treatments. In conclusion, this study provides comprehensive insights into developments and trends in sarcopenia research that can help researchers and clinicians better manage and implement their work.

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The role and therapeutic potential of stem cells in skeletal muscle in sarcopenia

Cited by 22 publications

References 120 publications

Macroporous Aligned Hydrogel Microstrands for 3D Cell Guidance

Macroporous Aligned Hydrogel Microstrands for 3D Cell Guidance

Decrease in the expression of muscle-specific miRNAs, miR-133a and miR-1, in myoblasts with replicative senescence

Bibliometric Analysis of the Knowledge Base and Future Trends on Sarcopenia from 1999–2021

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