The Role and Prevalence of Polyol Pathway and Oxidative Stress Markers as Risk Factors for Diabetic Cataract in Adult Type-I Diabetic and Diabetic Cataract Saudi Patients

Alwadani, Fahad; Saif, Musad

doi:10.4172/2155-9570.1000558

Cited by 2 publications

(1 citation statement)

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“…Accumulation of a high concentration of polyols in the lens leads to an increase in the intracellular ionic strength resulting in excessive hydration, eventually loss of membrane integrity, and leakage of free amino acids, glutathione, and myoinositol. AR catalyzes the reduction of glucose, galactose, and xylose into the corresponding sugar alcohol, sorbitol [ 15 ]. In the present study, chemical analysis of galactose treated lenses exhibited higher AR activity which was decreased by the treatment with ethanol extract of H. indicus var.…”

Section: Discussionmentioning

confidence: 99%

Repression of Polyol Pathway Activity by Hemidesmus indicus var. pubescens R.Br. Linn Root Extract, an Aldose Reductase Inhibitor: An In Silico and Ex Vivo Study

Haroon

Perumalsamy

Nair

et al. 2020

Nat. Prod. Bioprospect.

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Development of diabetic cataract is mainly associated with the accumulation of sorbitol via the polyol pathway through the action of Aldose reductase (AR). Hence, AR inhibitors are considered as potential agents in the management of diabetic cataract. This study explored the AR inhibition potential of Hemidesmus indicus var. pubescens root extract by in silico and ex vivo methods. Molecular docking studies (Auto Dock tool) between β-sitosterol, hemidesminine, hemidesmin-1, hemidesmin-2, and AR showed that β-sitosterol (− 10.2 kcal/mol) and hemidesmin-2 (− 8.07 kcal/mol) had the strongest affinity to AR enzyme. Ex vivo studies were performed by incubating isolated goat lenses in artificial aqueous humor using galactose (55 mM) as cataract inducing agent at room temperature (pH 7.8) for 72 h. After treatment with Vitamin E acetate − 100 µg/mL (standard) and test extract (500 and 1000 µg/mL) separately, the estimation of biochemical markers showed inhibition of lens AR activity and decreased sorbitol levels. Additionally, extract also normalized the levels of antioxidant markers like SOD, CAT, GSH. Our results showed evidence that H. indicus var. pubescens root was able to prevent cataract by prevention of opacification and formation of polyols that underlines its potential as a possible therapeutic agent against diabetic complications. Graphic Abstract

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Section: Discussionmentioning

confidence: 99%