The role and mechanism of KCa3.1 channels in human monocyte migration induced by palmitic acid

Ma, Xiao-Zhen; Pang, Zheng-Da; Wang, Junhong; Song, Zheng; Zhao, Limei; Du, Xiao‐Jun; Deng, Xingming

doi:10.1016/j.yexcr.2018.05.020

Cited by 15 publications

(10 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…1), including linolenic acid (56.78 %), sitostenone (7.77 %), cyclooctacosane (6.31 %), hexadecanoic acid (4.98 %), stigmasterol (4.35 %), multiflora-7,9(11)-dien-3β-ol (2.46 %), and pentacosane (2.33 %) (▶ Table 1). Some of these identified compounds have been reported to induce migration, proliferation, or collagen production in several cell types [25][26][27][28]. Hexadecanoic acid (also known as palmitic acid) showed proliferative activity in fibroblasts and keratinocytes [29,30].…”

Section: Resultsmentioning

confidence: 99%

Potential Beneficial Effects of Digitaria ciliaris Flower Absolute on the Wound Healing-Linked Activities of Fibroblasts and Keratinocytes

et al. 2020

View full text Add to dashboard Cite

Digitaria ciliaris is widely reported to be a problematic weed in agricultural areas and is mainly used as an indicator plant for the development of herbicides. However, its bioactivities on skin regeneration and wound healing have not been investigated. In the present study, we investigated the effects of D. ciliaris flower absolute on skin wound healing and skin regeneration-related events, that is, proliferation, migration, and collagen biosynthesis, in human fibroblasts and keratinocytes. For this study, we extracted absolute from the D. ciliaris flower by solvent extraction and identified the composition of D. ciliaris flower absolute using GC/MS analysis. We also tested the effect of D. ciliaris flower absolute in CCD986sk fibroblasts and/or HaCaT keratinocytes using the WST assay and 5-bromo-2′-deoxyuridine incorporation assay, Boyden chamber assay, ELISA, sprouting assay, and immunoblotting. GC/MS analysis of D. ciliaris flower absolute revealed that it contained 15 compounds. The absolute increased the proliferations of keratinocytes and fibroblasts and the migration of fibroblasts but did not affect cell viabilities. In addition, it enhanced the syntheses of type I and IV collagen in fibroblasts, but not in keratinocytes. The sprouting assay showed increased sprout outgrowth of fibroblasts. In addition, D. ciliaris flower absolute induced the phosphorylation of extracellular signal-regulated kinase 1/2 and p38 mitogen-activated protein kinase in fibroblasts. These results indicate that D. ciliaris flower absolute may promote skin wound healing/regeneration by inducing the proliferation, migration, and collagen synthesis of fibroblasts, as well as the proliferation of keratinocytes. Therefore, D. ciliaris flower absolute may be a potential natural source for cosmetic or pharmaceutical agents that promote skin wound healing/regeneration.

show abstract

Section: Resultsmentioning

confidence: 99%

Potential Beneficial Effects of Digitaria ciliaris Flower Absolute on the Wound Healing-Linked Activities of Fibroblasts and Keratinocytes

et al. 2020

View full text Add to dashboard Cite

show abstract

“…25 KCa3.1 activation helps to sustain calcium entry into the cell and is critical for the inflammatory response in macrophages. [26][27][28][29] KCa3.1-mediated elevation of intracellular calcium is essential for the production of inflammatory cytokines and chemokines by macrophages. 26 Most importantly, TRAM-34, a selective inhibitor of KCa3.1, dramatically reduces the expression of pro-inflammatory genes during macrophage polarization and inhibits macrophage differentiation toward the M1 phenotype.…”

Section: Introductionmentioning

confidence: 99%

KCa3.1 Inhibition of Macrophages Suppresses Inflammatory Response Leading to Endothelial Damage in a Cell Model of Kawasaki Disease

Zheng¹,

Tao²,

Liu³

et al. 2021

JIR

View full text Add to dashboard Cite

Purpose Macrophages-mediated inflammation is linked with endothelial damage of Kawasaki disease (KD). KCa3.1, a calcium-activated potassium channel, modulates inflammation of macrophages. However, little is known about the role of KCa3.1 in inflammation by macrophages involved in KD. Hence, this study is aimed to explore the potential role of KCa3.1 in regulating inflammatory response by macrophages and subsequent vascular injury in an in vitro model of KD. Methods RAW264.7 cells were stimulated with Lactobacillus casei cell wall extract (LCWE) with or without TRAM-34 or PDTC or AG490. Subsequently, mouse coronary artery endothelial cells (MCAECs) were incubated with RAW264.7 cells-conditioned medium to mimic local inflammatory lesions in KD. CCKi8 assay was used to evaluate cell viability. The mRNA levels of inflammatory mediators were detected by qRT-PCR. Expressions of KCa3.1, MCAECs injury-associated molecules, proteins involved in signal pathways of nuclear factor-κB (NF-κB), signal transducers and activators of transcription (STAT) 3 and p38 were evaluated by Western blot. Results Our study showed that LCWE increased KCa3.1 protein level in RAW264.7 macrophages and KCa3.1 inhibition by TRAM-34 notably suppressed the expression of pro-inflammatory molecules in LCWE-treated macrophages via blocking the activation of NF-κB and STAT3 pathways. Besides, the inflammation and damage of MCAECs were attenuated in the TRAM-34-treated group compared with the KD model group. This vascular protective role was dependent on the down-regulation of NF-κB and STAT3 signal pathways, which was confirmed by using inhibitors of NF-κB and STAT3. Conclusion This study demonstrates that KCa3.1 blockade of macrophages suppresses inflammatory reaction leading to mouse coronary artery endothelial cell injury in a cell model of KD by hampering the activation of NF-κB and STAT3 signaling pathway. These findings imply that KCa3.1 may be a potential therapeutic target for KD.

show abstract

“…Of these compounds, linolenic acid had the largest peak area (27.97 %) and was followed by tetracosane (–) (8.72 %), 1‐heneicosene (7.69 %), 10‐heneicosene (6.46 %), campesterol (6.35 %), 4‐methyl‐6‐ tert ‐butylphenol (5.77 %), clionasterol (5.60 %), and 1‐tetracosanol (5.15 %; Table 1). Of the 23 identified components in PFFA, hexadecanoic acid, linolenic acid, and vitamin E are reported to promote the migration, proliferation, or skin barrier associated protein formation in many cell types including keratinocytes [29–34] . In addition, cycloartenol, hexadecanoic acid, and vitamin E were implicated in inducing collagen synthesis in cells such as hepatic stellate cells or dermal fibroblasts [29,35–37] .…”

Section: Resultsmentioning

confidence: 99%

“…Of the 23 identified components in PFFA, hexadecanoic acid, linolenic acid, and vitamin E are reported to promote the migration, proliferation, or skin barrier associated protein formation in many cell types including keratinocytes. [29][30][31][32][33][34] In addition, cycloartenol, hexadecanoic acid, and vitamin E were implicated in inducing collagen synthesis in cells such as hepatic stellate cells or dermal fibroblasts. [29,[35][36][37] Therefore, it can be assumed that PFFA may have the ability to facilitate healing and barrier repair-linked responses in wounded skin.…”

Section: Chemical Composition Of Pffamentioning

confidence: 99%

Stimulatory Effects of Paederia foetida Flower Absolute on the Skin Wound and Barrier Repair Activities of Keratinocytes

Kim

Won

Kim

et al. 2020

Chemistry & Biodiversity

View full text Add to dashboard Cite

Paederia foetida (PF) has antidiarrheal, antidiabetic, and anti-inflammatory activities. However, its biological activities on skin remain unclear. In this study, we examined the effect of PF flower absolute (PFFA) on skin wound healing-and skin barrier-linked responses in human epidermal keratinocytes (HaCaT cells). PFFA contained 23 components and increased the proliferation and sprout outgrowth of HaCaT cells and modestly increased migration. PFFA enhanced the phosphorylation levels of extracellular signal-regulated kinase1/2, serine/threonine-specific protein kinase (AKT), and p38 mitogen-activated protein kinase (MAPK) in HaCaT cells, and upregulated type I and IV collagen synthesis and filaggrin (an epidermal barrier protein) expression in HaCaT cells. These findings suggest PFFA may promote skin wound repair by stimulating migratory and proliferative activities (probably through the AKT/MAPK pathway), collagen synthesis, and skin barrier repair by upregulating the expressions of filaggrin in epidermal keratinocytes. Therefore, PFFA may be useful for developing agents that enhance skin wound and barrier-repair functions.

show abstract

The role and mechanism of KCa3.1 channels in human monocyte migration induced by palmitic acid

Cited by 15 publications

References 33 publications

Potential Beneficial Effects of Digitaria ciliaris Flower Absolute on the Wound Healing-Linked Activities of Fibroblasts and Keratinocytes

Potential Beneficial Effects of Digitaria ciliaris Flower Absolute on the Wound Healing-Linked Activities of Fibroblasts and Keratinocytes

KCa3.1 Inhibition of Macrophages Suppresses Inflammatory Response Leading to Endothelial Damage in a Cell Model of Kawasaki Disease

Stimulatory Effects of Paederia foetida Flower Absolute on the Skin Wound and Barrier Repair Activities of Keratinocytes

Contact Info

Product

Resources

About