The role and mechanism of microRNA‑18a‑5p in oral squamous cell carcinoma

Huang, Chenyao; Song, Hongning; Lai, Linfeng

doi:10.3892/mmr.2019.10403

Cited by 11 publications

(11 citation statements)

References 24 publications

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“…A possible explanation to these contrasting reports regarding the expression and biological role of miR-18a is that depending on the tumor cell type, miR-18a regulates a particular group of genes, some with oncogenic roles and others with tumor-suppressive capabilities. For example, Huang et al observed that Smad2, a downstream effector of the TGFb signaling and a potent tumor suppressor in several cancers, is a major target of miR-18a in oral squamous cell carcinoma (34). The tumor suppressor genes PTEN and IRF2 have also been reported as direct targets of miR-18a in osteosarcoma (35,36).…”

Section: Discussionmentioning

confidence: 99%

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MicroRNA-18a-5p Suppresses Tumor Growth via Targeting Matrix Metalloproteinase-3 in Cisplatin-Resistant Ovarian Cancer

et al. 2020

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Cumulating evidence indicates that dysregulation of microRNAs (miRNAs) plays a central role in the initiation, progression, and drug resistance of cancer cells. However, the specific miRNAs contributing to drug resistance in ovarian cancer cells have not been fully elucidated. Aimed to identify potential miRNAs involved in platinum resistance, we performed a miRNA expression profile in cisplatin-sensitive and cisplatin-resistant ovarian cancer cells, and we found several differentially abundant miRNAs in the pair of cell lines. Notably, miR-18a-5p (miR-18a), a member of the oncogenic associated miR-17-92 cluster, was decreased in cisplatin-resistant as compared with cisplatin-sensitive cells. Real-time PCR analysis confirmed these findings. We then studied the biological, molecular, and therapeutic consequences of increasing the miR-18a levels with oligonucleotide microRNA mimics (OMM). Compared with a negative control OMM, transient transfection of a miR-18a-OMM reduced cell growth, cell proliferation, and cell invasion. Intraperitoneal injections of miR-18a-OMM-loaded folate-conjugated liposomes significantly reduced the tumor weight and the number of nodules in ovarian cancer-bearing mice when compared with a control-OMM group. Survival analysis using the Kaplan-Meier plotter database showed that ovarian cancer patients with high miR-18a levels live longer in comparison to patients with lower miR-18a levels. Bioinformatic analyses, real-time-PCR, Western blots, and luciferase reporter assays revealed that Matrix Metalloproteinase-3 (MMP-3) is a direct target of miR-18a. Small-interfering RNA (siRNA)-mediated silencing of MMP-3 reduced cell viability, cell growth, and the invasiveness potential of cisplatin-resistant ovarian cancer cells. Our study suggests that targeting miR-18a is a plausible therapeutic strategy for cisplatin-resistant ovarian cancer.

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Section: Discussionmentioning

confidence: 99%

“…For example, Huang et al. observed that Smad2, a downstream effector of the TGF-β signaling and a potent tumor suppressor in several cancers, is a major target of miR-18a in oral squamous cell carcinoma ( 34 ). The tumor suppressor genes PTEN and IRF2 have also been reported as direct targets of miR-18a in osteosarcoma ( 35 , 36 ).…”

Section: Discussionmentioning

confidence: 99%

MicroRNA-18a-5p Suppresses Tumor Growth via Targeting Matrix Metalloproteinase-3 in Cisplatin-Resistant Ovarian Cancer

et al. 2020

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“…In the TGF-β1 pathway, Smad2 and Smad3 are receptor-regulated effector proteins, and TGF-β induces apoptosis through Smad-mediated expression of death-associated protein kinase ( 141 ). Recently, Huang and colleagues ( 142 ) investigated the role and mechanism of miR−18a-5p in OSCC and it was revealed that miR−18a-5p was highly expressed in SCC9 cells compared with normal cells. Then the effect of miR−18a-5p upregulation and miR−18a-5p downregulation was investigated on SCC9 cell viability, migration, invasion and apoptosis.…”

Section: Biogenesis and Function Of Micrornas And Their Roles In Prog...mentioning

confidence: 99%

Non-Coding RNAs and Oral Cancer: Small Molecules With Big Functions

2022

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Oral cancer remains a major public concern with considerable socioeconomic impact in the world. Despite substantial advancements have been made in treating oral cancer, the five-year survival rate for oral cancer remained undesirable, and the molecular mechanisms underlying OSCC carcinogenesis have not been fully understood. Noncoding RNAs (ncRNAs) include transfer RNAs (tRNAs), as well as small RNAs such as microRNAs, and the long ncRNAs such as HOTAIR are a large segment of the transcriptome that do not have apparent protein-coding roles, but they have been verified to play important roles in diverse biological processes, including cancer cell development. Cell death, such as apoptosis, necrosis, and autophagy, plays a vital role in the progression of cancer. A better understanding of the regulatory relationships between ncRNAs and these various types of cancer cell death is therefore urgently required. The occurrence and development of oral cancer can be controlled by increasing or decreasing the expression of ncRNAs, a method which confers broad prospects for oral cancer treatment. Therefore, it is urgent for us to understand the influence of ncRNAs on the development of different modes of oral tumor death, and to evaluate whether ncRNAs have the potential to be used as biological targets for inducing cell death and recurrence of chemotherapy. The purpose of this review is to describe the impact of ncRNAs on cell apoptosis and autophagy in oral cancer in order to explore potential targets for oral cancer therapy.

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“…It plays an important role in deregulation of malignancies [ 6–8 ], which is closely associated with oncogene and progression of tumors through affecting the cellular process, such as cell growth and metastasis [ 9 , 10 ]. Hence, miRNAs are considered as important biomarkers and therapeutic targets in several types of cancers [ 11 , 12 ]. In NSCLC, the miR-199a-5p was reported to be targeted with MAP3K11 to inhibit cell progression [ 13 ].…”

Section: Introductionmentioning

confidence: 99%

miR-371b-5p promotes cell proliferation, migration and invasion in non-small cell lung cancer via SCAI

et al. 2020

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Objective: Multiple gene targets have been reported for treatment of non-small cell lung cancer (NSCLC), however, the accompanying genetic tolerance was reported increasingly. Therefore, it is important to find new biomarkers or therapeutic targets in treatment of NSCLC. Methods: The expression levels of miR-371b-5p were detected by qRT-PCR in NSCLC tissues and cell lines. To evaluate the effect of miR-371b-5p on NSCLC progression, we firstly transfected the miR-371b-5p inhibitor for construction of the miR-371b-5p down-regulated cell model. Then the cell proliferation, migration, invasion and cell apoptosis were detected. In addition, the expression levels of adhesion factors were detected. The target gene of miR-371b-5p was identified by bioinformatics analysis, and rescue experiment was conducted to validate the effect of miR-371b-5p on proliferation, migration and invasion of NSCLC. Results: Our findings revealed that the miR-371b-5p was overexpressed in NSCLC and could markedly promote the cell proliferation, migration and invasion. Expression levels of both ICAM-1 and VCAM-1 were significantly down-regulated when treated by miR-371b-5p inhibitor. Moreover, dual-luciferase reporter assay showed that the miR-371b-5p targeted SCAI in regulation of cell proliferation, migration and invasion, and the expression of miR-371b-5p was negatively associated with SCAI in NSCLC tissues and cell lines. Rescue experiment revealed that the miR-371b-5p could rescue the effect of SCAI on cell proliferation, migration and invasion. Conclusion: Our results suggest that the miR-371b-5p and SCAI may serve as novel prognostic biomarkers and therapeutic targets for NSCLC.

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The role and mechanism of microRNA‑18a‑5p in oral squamous cell carcinoma

Cited by 11 publications

References 24 publications

MicroRNA-18a-5p Suppresses Tumor Growth via Targeting Matrix Metalloproteinase-3 in Cisplatin-Resistant Ovarian Cancer

MicroRNA-18a-5p Suppresses Tumor Growth via Targeting Matrix Metalloproteinase-3 in Cisplatin-Resistant Ovarian Cancer

Non-Coding RNAs and Oral Cancer: Small Molecules With Big Functions

miR-371b-5p promotes cell proliferation, migration and invasion in non-small cell lung cancer via SCAI

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