The Role and Efficacy of Herbal Antimicrobial Agents in Orthodontic Treatment

Mishra, Sujata; Routray, Samapika; Sahu, Sanjeeb Kumar; Nanda, Sarita; Sahu, Kanhu Charan

doi:10.7860/jcdr/2014/7349.4464

Cited by 5 publications

(3 citation statements)

References 17 publications

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“…Studies of its antimicrobial effects have been performed in various aspects in vitro such as to evaluate its efficacy as an endodontic irrigant, for orthodontic wires [30] and in cultures. When compared with sodium hypochlorite triphala showed promising results [31,32] Active ingredients like tannins, which restrict adhesion; quinines which provide free radicals and cause inactivation of microbial proteins and flavones, flavonoids, and flavonols, which disrupt microbial membrane and cell walls contribute to its antimicrobial its properties [28].…”

Section: Discussionmentioning

confidence: 99%

Comparative Evaluation of Antimicrobial Effects of Herbal Extracts Incorporated in Dentifrices among Adults: An In-vitro Study and a Double Blinded Randomized Control Trial

Kalra¹,

Anjum

Alam

et al. 2022

JPRI

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Introduction: A positive correlation has been observed between Streptococcus mutans, Candida albicans and periodontal diseases. Several substances including synthetic chemicals and natural agents have shown promising antimicrobial activity against the S. mutans- C. albicans complex. Extracts of Aloe Vera, Triphala, Tulsi, Pomegranate, and Acacia catechu have established therapeutic effects in medicine. The aim of the study was to evaluate the antimicrobial activity and the Minimum Inhibitory Concentration (MIC) of five herbal extracts in-vitro and to compare the antibacterial effects of 5 dentifrices containing the herbal extracts and a commercially available dentifrice on the plaque sample counts of S. mutans and C. albicans in-vivo Methodology: Extracts were obtained using a standardized method. Minimum inhibitory concentration was calculated for the test microorganisms using modified agar-well diffusion and macro broth dilution technique and the inhibition zone diameters were calculated. Dentifrices containing the extracts were formulated and the in vivo antimicrobial activity was evaluated by studying the CFU count from plaque samples of 420 participants. Results: Highest antimicrobial activity against S Mutans and C albicans was shown by Acacia catechu and Tulsi respectively and least was by pomegranate. Mean reduction in microbial counts (CFU/ml) by inoculating nutrient broth from baseline to 60 days was highest in Acacia catechu and Triphala for S Mutans and C albicans respectively. All herbal extracts performed better than the commercially available toothpaste. Conclusion: Dentifrices with herbal extracts of Aloe Vera, Triphala, Tulsi, Pomegranate, and Acacia catechu could serve as an alternative to commercially available dentifrices for treating oral diseases whose causative organisms are S. mutans and C. albicans.

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Section: Discussionmentioning

confidence: 99%

Comparative Evaluation of Antimicrobial Effects of Herbal Extracts Incorporated in Dentifrices among Adults: An In-vitro Study and a Double Blinded Randomized Control Trial

Kalra¹,

Anjum

Alam

et al. 2022

JPRI

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“…Orthodontic treatment is a prolonged process that addresses a patient's aesthetic and functional teeth problems. [1][2][3] Orthodontic treatment for managing malocclusion is divided into removable and fixed orthodontic applications. [4][5][6] Treatment using a fixed orthodontic appliance reduces oral hygiene and increases biofilm retention area.…”

Section: Introductionmentioning

confidence: 99%

“…11,13,14,16,18 S. mutans bacteria are the main cause of caries, as it plays a vital role in biofilm formation. 1,3,19,20 There are two adhesion mechanisms for S. mutans, namely, sucroseindependent adhesion and sucrose-dependent adhesion. Sucrose-independent adhesion is a mechanism through which extracellular sucrose is transformed into glucan by cell wall-associated glucosyltransferase in the presence of sucrose and, together with glucan binding protein on the bacterial surface, promotes cells aggregation and the subsequent development of dental biofilms on the tooth surface.…”

Section: Introductionmentioning

confidence: 99%

Mobe leaf (Artocarpus lakoocha Buch. Ham) ethanol extract’s antibacterial activity on Streptococcus mutans cell membrane leakage and biofilm formation: An in vitro study

Bahirrah,

Satria,

Yuwanda

2023

Dent. J.

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Background: Using fixed orthodontic appliances inhibits oral hygiene, which can lead to the increased development of biofilms and Streptococcus mutans, a cariogenic bacterium that is well known for causing dental caries, derived from bacteria of the Streptococcus genus. In order to decrease biofilm and the degree of cariogenic bacteria in the oral cavity, a variety of herbal ingredients are used. Among these, mobe (Artocarpus lakoocha Buch. Ham) leaves are herbal ingredients with antibacterial properties. Purpose: This study aimed to investigate the antibacterial activity, antibiofilm, and leakage of DNA and protein from mobe leaves. Methods: The diffusion method was used to assess antibacterial activity and determine the minimum inhibitory concentration. The antibiofilm activity was evaluated with Ultraviolet–visible (UV-Vis) spectrophotometry (600 nm), using violet crystal staining. The detection of DNA and protein leakage was carried out by checking for absorbance values using the UV-Vis spectrophotometry (260 nm and 280 nm). An increase in the absorbance value in the measured cell indicated an increase in the level of cell content produced by the cell. One-way analysis of variance was used statistically analyze the results of this study (P < 0.05). Results: Mobe leaf (A. lakoocha) extract’s minimum inhibitory concentration level was 3.125 mg/ml, the effective concentration of ethanol extract for inhibiting biofilm formation was 3.125 mg/ml, and the effective concentration of ethanol extract that could cause DNA and protein leakage was 50 mg/ml. Conclusions: Mobe leaf extract has good MIC for S. mutans.

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The antimicrobial effect of different vitamin D compounds on Streptococcus mutans and their impact on glycosyltransferase expression

Picolo,

Stephen,

Baysan

2024

Journal of Oral Microbiology

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Background Streptococcus mutans is a virulent microorganism associated with dental caries. This in vitro study aimed to investigate the antimicrobial effects of Cholecalciferol (D3) and Doxercalciferol (D2), against S. mutans and on glycosyltransferase gene expression. Methods Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of D3 and D2 for S. mutans were determined according to the Clinical Laboratory Standards Institute guidelines. The effect of the compounds on environmental pH in 1% w/v and 5% w/v sucrose broth cultures after 24 hours were assessed colorimetrically. Additionally, their impact on glycosyltransferases gene expression ( GtfB, GtfC, GtfD ) in 5% w/v sucrose culture was evaluated using quantitative real-time PCR. Results The MBCs of D3 and D2 were 83 µg/ml and 166 µg/ml respectively. Both compounds were effective in preventing the local pH drop <5.5 at ≥166 µg/ml in sucrose supplemented cultures. However, the compounds did not inhibit pH drop at MIC values. Notably, D2 upregulated GtfD expression significantly ( p < 0.05) and downregulated GtfB and GtfC . Conclusion Vitamin D2 and D3 inhibited S. mutans mediated pH drop in sucrose supplemented cultures and altered glycosyltransferase expression, suggesting potential therapeutic roles in dental caries prevention. Further research is needed to assess their full impact on S. mutans survival under environmental stresses.

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The Role and Efficacy of Herbal Antimicrobial Agents in Orthodontic Treatment

Cited by 5 publications

References 17 publications

Comparative Evaluation of Antimicrobial Effects of Herbal Extracts Incorporated in Dentifrices among Adults: An In-vitro Study and a Double Blinded Randomized Control Trial

Comparative Evaluation of Antimicrobial Effects of Herbal Extracts Incorporated in Dentifrices among Adults: An In-vitro Study and a Double Blinded Randomized Control Trial

Mobe leaf (Artocarpus lakoocha Buch. Ham) ethanol extract’s antibacterial activity on Streptococcus mutans cell membrane leakage and biofilm formation: An in vitro study

The antimicrobial effect of different vitamin D compounds on Streptococcus mutans and their impact on glycosyltransferase expression

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