The RNA world of plant mitochondria

Hoffmann, Michaela; Kuhn, Josef; Däschner, Klaus; Binder, Stefan

doi:10.1016/s0079-6603(01)70015-3

Cited by 22 publications

(8 citation statements)

References 143 publications

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“…Many mRNA termini have been mapped (Kü hn et al, 2005;Forner et al, 2007), but, apart from a few exceptions, neither the cis-acting sequence elements nor the trans-factors required for the generation of these ends have as yet been identified (Hoffmann et al, 2001;Gagliardi and Binder, 2007). Because biochemical approaches are very difficult or even impossible, mainly genetic approaches have been applied to study components of RNA maturation.…”

Section: Natural Genetic Variation: a Promising Tool To Investigate Mmentioning

confidence: 99%

Mitochondrial mRNA Polymorphisms in Different Arabidopsis Accessions

et al. 2008

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In our analysis of 5# and 3# end formation in plant mitochondria, we compared the major transcript ends of all mitochondrial protein-coding genes between the three Arabidopsis (Arabidopsis thaliana) accessions Columbia (Col), C24, and Landsberg erecta (Ler). Differences between transcript patterns were found for seven genes. For atp6-2, no transcripts at all were detected in Ler. This and further analyses suggest that the atp6-2 gene arrangement is absent from the mitochondrial DNA of this accession. All other transcript polymorphisms are attributed to variations at the 5# termini and were consistently observed in all tissues investigated. mRNA phenotyping of reciprocal Col/Ler, Col/C24, and Ler/C24 F 1 hybrids revealed the differing transcript patterns of ccmC to be inherited maternally, suggesting these to arise from differences in the mitochondrial DNA. Biparental inheritance was observed for the polymorphic transcripts of nad4, nad9, ccmB, and rpl5, indicating these differences to be caused by nuclear-encoded trans-factors. Deviant transcript patterns were tested in further accessions and were found in at least three additional accessions. Detailed examination of the nad4 and the nad9 transcripts demonstrates that the respective polymorphisms affect the major mRNAs of these genes. This study shows that natural genetic variation in Arabidopsis can also affect mitochondrial mRNA end processing. These variations can now be used to identify the nuclear genes responsible, as well as the mitochondrial cis-elements required, for 5# end generation of mitochondrial transcripts.

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Section: Natural Genetic Variation: a Promising Tool To Investigate Mmentioning

confidence: 99%

Mitochondrial mRNA Polymorphisms in Different Arabidopsis Accessions

et al. 2008

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“…For expression of this organellar genetic information a large set of proteins is required. This includes many different polypeptides for posttranscriptional processes such as splicing, RNA editing as well as 5 0 and 3 0 end maturation of rRNA, tRNA and mRNA (Hoffmann et al 2001;Binder and Brennicke 2003;Marchfelder and Binder 2004;Gagliardi and Binder 2007;Bonen 2008;Takenaka et al 2008). In recent years a number of proteins involved in these posttranscriptional processes have been identified, however, the vast majority of the factors required for mitochondrial gene expression is still unknown.…”

Section: Introductionmentioning

confidence: 99%

The DEAD-box protein PMH2 is required for efficient group II intron splicing in mitochondria of Arabidopsis thaliana

2009

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In Arabidopsis thaliana the putative mitochondrial RNA helicases PMH1 and PMH2 are members of the large DEAD-box protein family. Our previous characterization of these proteins revealed that PMH1 and/or PMH2 are part of high molecular weight complexes. Now T-DNA insertion lines were established and characterized for each of these genes. Immunodetection analysis of cell suspension cultures established from pmh1-1 and pmh2-1 mutants revealed that indeed both DEAD-box proteins are detectable in large protein complexes with PMH2 being much more abundant than PMH1. In plants the knockout of PMH2 leads to reduced group II intron splicing efficiency. In addition the steady-state levels of several mature mitochondrial mRNAs are decreased while transcription is not influenced. This molecular phenotype suggests that PMH2 acts at the posttranscriptional level with a potential function as RNA chaperone required for formation or maintenance of complex RNA secondary structures of introns rather than a direct role in splicing. In contrast, the investigation of a pmh1-1 knockout line did not reveal any influence of this protein on processing and abundance of mitochondrial transcripts.

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“…After transcription, primary RNAs undergo a series of processing steps. For instance, many genes are transcribed into polycistronic precursor RNAs that become disassembled into smaller units (9). Apart from intron splicing, 5′ and 3′ ends are post-transcriptionally generated.…”

Section: Introductionmentioning

confidence: 99%

Distant sequences determine 5' end formation of cox3 transcripts in Arabidopsis thaliana ecotype C24

Forner¹,

Weber²,

Wiethölter³

et al. 2005

Nucleic Acids Research

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The genomic environments and the transcripts of the mitochondrial cox3 gene are investigated in three Arabidopsis thaliana ecotypes. While the proximate 5′ sequences up to nucleotide position −584, the coding regions and the 3′ flanking regions are identical in Columbia (Col), C24 and Landsberg erecta (Ler), genomic variation is detected in regions further upstream. In the mitochondrial DNA of Col, a 1790 bp fragment flanked by a nonanucleotide direct repeat is present beyond position −584 with respect to the ATG. While in Ler only part of this insertion is conserved, this sequence is completely absent in C24, except for a single copy of the nonanucleotide direct repeat. Northern hybridization reveals identical major transcripts in the three ecotypes, but identifies an additional abundant 60 nt larger mRNA species in C24. The extremities of the most abundant mRNA species are identical in the three ecotypes. In C24, an extra major 5′ end is abundant. This terminus and the other major 5′ ends are located in identical sequence regions. Inspection of Atcox3 transcripts in C24/Col hybrids revealed a female inheritance of the mRNA species with the extra 5′ terminus. Thus, a mitochondrially encoded factor determines the generation of an extra 5′ mRNA end.

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The RNA world of plant mitochondria

Cited by 22 publications

References 143 publications

Mitochondrial mRNA Polymorphisms in Different Arabidopsis Accessions

Mitochondrial mRNA Polymorphisms in Different Arabidopsis Accessions

The DEAD-box protein PMH2 is required for efficient group II intron splicing in mitochondria of Arabidopsis thaliana

Distant sequences determine 5' end formation of cox3 transcripts in Arabidopsis thaliana ecotype C24

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