The RNA polymerase II subunit NRPB2 is required for indeterminate root development, cell viability, stem cell niche maintenance, and de novo root tip regeneration in Arabidopsis

Raya‐González, Javier; Ávalos-Rangel, Adrián; Ruíz-Herrera, León Francisco; Valdéz-Alarcón, Juan José; López‐Bucio, José

doi:10.1007/s00709-021-01732-z

Cited by 4 publications

(3 citation statements)

References 43 publications

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“…DNA damage induced by genotoxic agents or specific gene mutations affects mitotic activity in the root meristem through cell cycle‐related gene expression (Raya‐González et al, 2022; Raya‐González et al, 2018). To assess if root tip exhaustion in phyA‐211 as a response to zeocin could be associated to defects on cell cycle progression in the root meristem, we analysed the expression of CycB1;1 in WT and phyA‐211 introducing the CycB1;1:uidA gene construct into phyA‐211 homozygous mutants via outcrossing.…”

Section: Resultsmentioning

confidence: 99%

PHYTOCHROME A controls the DNA damage response and cell death tolerance within the Arabidopsis root meristem

Huerta‐Venegas,

Raya‐González,

Ruíz‐Herrera

et al. 2024

Plant Cell & Environment

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The DNA damage response avoids mutations into dividing cells. Here, we analysed the role of photoreceptors on the restriction of root growth imposed by genotoxic agents and its relationship with cell viability and performance of meristems. Comparison of root growth of Arabidopsis WT, phyA‐211, phyB‐9, and phyA‐211phyB‐9 double mutants unveiled a critical role for phytochrome A (PhyA) in protecting roots from genotoxic stress, regeneration and cell replenishment in the meristematic zone. PhyA was located on primary root tips, where it influences genes related to the repair of DNA, including ERF115 and RAD51. Interestingly, phyA‐211 mutants treated with zeocin failed to induce the expression of the repressor of cell cycle MYB3R3, which correlated with expression of the mitotic cyclin CycB1, suggesting that PhyA is required for safeguarding the DNA integrity during cell division. Moreover, the growth of the primary roots of PhyA downstream component HY5 and root growth analyses in darkness suggest that cell viability and DNA damage responses within root meristems may act independently from light and photomorphogenesis. These data support novel roles for PhyA as a key player for stem cell niche maintenance and DNA damage responses, which are critical for proper root growth.

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Section: Resultsmentioning

confidence: 99%

PHYTOCHROME A controls the DNA damage response and cell death tolerance within the Arabidopsis root meristem

Huerta‐Venegas,

Raya‐González,

Ruíz‐Herrera

et al. 2024

Plant Cell & Environment

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“…CenRNAs are present in either monomeric or multimeric repeats in various species (Table 1). Human centromeric transcripts of approximately 1.3 kb have been previously identified (Quenet & Dalal, 2014). In Drosophila, long noncoding transcripts are generated from SATIII (359 bp) repeats and have been suggested to be crucial for correct mitotic chromosome segregation (Ro si c et al, 2014).…”

Section: Centromeric Transcripts and Their Role In Kinetochore Assemblymentioning

confidence: 99%

“…In eukaryotes, three conserved multi‐subunit RNA polymerases (RNA Pol I, II, and III) are required for the transcription of nuclear genomes into different coding and non‐coding RNA (Raya‐González et al., 2022; Szcześniak et al., 2016). Additionally, two plant‐specific RNA polymerases (RNA Pol IV and V), have been identified (Wierzbicki et al., 2008).…”

Section: Rna Polymerases In Transcription Of Centromeric Repeatsmentioning

confidence: 99%

The role of centromeric repeats and transcripts in kinetochore assembly and function

Ramakrishnan Chandra,

Kalidass,

Demidov

et al. 2023

The Plant Journal

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SUMMARYCentromeres are the chromosomal domains, where the kinetochore protein complex is formed, mediating proper segregation of chromosomes during cell division. Although the function of centromeres has remained conserved during evolution, centromeric DNA is highly variable, even in closely related species. In addition, the composition of the kinetochore complexes varies among organisms. Therefore, it is assumed that the centromeric position is determined epigenetically, and the centromeric histone H3 (CENH3) serves as an epigenetic marker. The loading of CENH3 onto centromeres depends on centromere‐licensing factors, chaperones, and transcription of centromeric repeats. Several proteins that regulate CENH3 loading and kinetochore assembly interact with the centromeric transcripts and DNA in a sequence‐independent manner. However, the functional aspects of these interactions are not fully understood. This review discusses the variability of centromeric sequences in different organisms and the regulation of their transcription through the RNA Pol II and RNAi machinery. The data suggest that the interaction of proteins involved in CENH3 loading and kinetochore assembly with centromeric DNA and transcripts plays a role in centromere, and possibly neocentromere, formation in a sequence‐independent manner.

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The Transcriptional MEDIATOR Complex: Linking Root Development, Hormonal Responses, and Nutrient Stress

Raya-González,

López-Bucio,

López-Bucio

2023

J Plant Growth Regul

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The RNA polymerase II subunit NRPB2 is required for indeterminate root development, cell viability, stem cell niche maintenance, and de novo root tip regeneration in Arabidopsis

Cited by 4 publications

References 43 publications

PHYTOCHROME A controls the DNA damage response and cell death tolerance within the Arabidopsis root meristem

PHYTOCHROME A controls the DNA damage response and cell death tolerance within the Arabidopsis root meristem

The role of centromeric repeats and transcripts in kinetochore assembly and function

The Transcriptional MEDIATOR Complex: Linking Root Development, Hormonal Responses, and Nutrient Stress

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