The RNA polymerase I transcription machinery

Russell, Jackie; Zomerdijk, Joost C.B.M.

doi:10.1042/bss0730203

Cited by 147 publications

(119 citation statements)

References 99 publications

Supporting

Mentioning

116

Contrasting

Unclassified

Order By: Relevance

“…Hence, one major function of TFIID and CRSP/Med in the progenitor, and even in transformed cells, may be to promote transcription of positive cell cycle regulators, and, more importantly, their near complete loss in differentiated cells may be necessary to ensure that these genes are turned off, allowing committed cells to withdraw from the cell cycle. Furthermore, transcription by pol I and pol III requires TBP, and we have yet to understand how differentiated cells with reduced TBP concentrations maintain steady-state transcription by these essential RNA polymerases (41,42). Finally, the extracellular events and signaling cascades which induce developmental changes in TFIID and CRSP/Med must be uncovered if we are to fully appreciate the dynamics of these factors during a given developmental transition.…”

Section: Discussionmentioning

confidence: 99%

Core promoter recognition complex changes accompany liver development

D’Alessio

Willenbring

et al. 2011

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

Recent studies of several key developmental transitions have brought into question the long held view of the basal transcriptional apparatus as ubiquitous and invariant. In an effort to better understand the role of core promoter recognition and coactivator complex switching in cellular differentiation, we have examined changes in transcription factor IID (TFIID) and cofactor required for Sp1 activation/Mediator during mouse liver development. Here we show that the differentiation of fetal liver progenitors to adult hepatocytes involves a wholesale depletion of canonical cofactor required for Sp1 activation/Mediator and TFIID complexes at both the RNA and protein level, and that this alteration likely involves silencing of transcription factor promoters as well as protein degradation. It will be intriguing for future studies to determine if a novel and as yet unknown core promoter recognition complex takes the place of TFIID in adult hepatocytes and to uncover the mechanisms that down-regulate TFIID during this critical developmental transition.TATA box-binding protein-associated factor | hepatoblast | hepatogenesis T he precise and orderly differentiation of embryonic progenitors to committed adult cell types requires exquisite spatial and temporal control of gene expression. Until recently, the dynamic changes in transcriptional output that accompany developmental transitions were assumed to depend exclusively on regulatory DNA and its associated sequence-specific activators and repressors, whereas core promoter recognition, coactivator, and chromatin modifier complexes were generally taken to be ubiquitous and invariant from one cell type to the next. Indeed, these so-called core factors are highly conserved from yeast to man (1). However, recent evidence suggests that several key developmental transitions are accompanied by, and actually require, dramatic changes in components of this general machinery including transcription factor IID (TFIID) composed of the TATA box-binding protein (TBP) and its associated factors (TAFs), the cofactor required for Sp1 activation/Mediator (CRSP/Med) complex, and the Brg1/Brm-associated factor (BAF) complex (for review, see 2).The first evidence that some cell types may contain alterations of the general machinery came with the identification of germcell-specific TFIID subcomplexes in which key subunits are replaced by paralogous components. Initially oogenesis and spermatogenesis were found to require an altered TFIID in which paralogous TAF4b replaces the canonical TAF4 (3, 4). Later spermatocytes were found to also express paralogous TAF7L that may cooperate with TBP and

show abstract

Section: Discussionmentioning

confidence: 99%

Core promoter recognition complex changes accompany liver development

D’Alessio

Willenbring

et al. 2011

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

show abstract

“…7,8 The essential and reversible interaction of the enzyme with Rrn3 is a critical event for Pol I transcription (reviewed in refs. [9][10][11], and has been shown to be a prime target for the regulation of the Pol I activity. 8,12,13 To interfere with these mechanisms, we constructed a yeast strain, named CARA (for Constitutive Association of Rrn3 and A43), in which the endogenous, essential Rrn3 factor and the A43 subunit of the Pol I that interacts with Rrn3, 14 were supplied as an Rrn3-A43 fusion protein.…”

Section: The Cara Strain or How To Make Pol I Transcription Constitumentioning

confidence: 99%

Is Ribosome Synthesis Controlled by Pol I Transcription?

Chédin¹,

Laferté²,

Hoang³

et al. 2007

Cell Cycle

View full text Add to dashboard Cite

“…Received December 11, 2007; revised version accepted February 29, 2008. In the yeast Saccharomyces cerevisiae (hereafter called yeast), >60% of total cellular transcripts are produced from the ribosomal DNA (rDNA) locus by a specific multiprotein enzyme, RNA Polymerase I (Pol I), and an assorted set of additional transcription factors (Reeder 1999;Moss and Stefanovsky 2002;Russell and Zomerdijk 2006). The basal Pol I transcription machinery has been largely defined and consists of two multiprotein complexes, the upstream activating factor (UAF) , and the core factor (CF) (Keys et al 1994), as well as the TATA-box-binding protein (TBP) (Steffan et al 1996).…”

mentioning

confidence: 99%

Actively transcribed rRNA genes in S. cerevisiae are organized in a specialized chromatin associated with the high-mobility group protein Hmo1 and are largely devoid of histone molecules

Merz¹,

Hondele²,

Goetze³

et al. 2008

Genes Dev.

169

215

View full text Add to dashboard Cite

Synthesis of ribosomal RNAs (rRNAs) is the major transcriptional event in proliferating cells. In eukaryotes, ribosomal DNA (rDNA) is transcribed by RNA polymerase I from a multicopy locus coexisting in at least two different chromatin states. This heterogeneity of rDNA chromatin has been an obstacle to defining its molecular composition. We developed an approach to analyze differential protein association with each of the two rDNA chromatin states in vivo in the yeast Saccharomyces cerevisiae. We demonstrate that actively transcribed rRNA genes are largely devoid of histone molecules, but instead associate with the high-mobility group protein Hmo1.[Keywords: Chromatin; transcription; ribosomal DNA] Supplemental material is available at http://www.genesdev.org. Received December 11, 2007; revised version accepted February 29, 2008. In the yeast Saccharomyces cerevisiae (hereafter called yeast), >60% of total cellular transcripts are produced from the ribosomal DNA (rDNA) locus by a specific multiprotein enzyme, RNA Polymerase I (Pol I), and an assorted set of additional transcription factors (Reeder 1999;Moss and Stefanovsky 2002;Russell and Zomerdijk 2006). The basal Pol I transcription machinery has been largely defined and consists of two multiprotein complexes, the upstream activating factor (UAF) , and the core factor (CF) (Keys et al. 1994), as well as the TATA-box-binding protein (TBP) (Steffan et al. 1996).The template of RNA Pol I transcription, the yeast ribosomal RNA (rRNA) genes are located on the right arm of chromosome XII in a tandem array of 150-200 copies (Fig. 1), representing almost 10% of the yeast genome. Each of the repeats contains the Pol I-transcribed 35S rRNA gene (precusor of the 5.8S, 18S, and 25S rRNAs) and the RNA Polymerase III (Pol III)-transcribed 5S rRNA gene (Fig. 1). Three different regulatory DNA elements have been identified within the 35S rRNA gene sequence, two of which are located at the 5Ј end of the 35S rDNA, spanning only ∼170 base pairs (bp) (Musters et al. 1989;Kulkens et al. 1991): the upstream element (UE), which is the binding site for the UAF; and the core promoter (CP), where CF can be recruited (Fig. 1). The third element, called the enhancer (ENH), is located at the 3Ј end of the 35S transcription unit (Fig. 1) and was originally identified as exhibiting a stimulatory effect on Pol I transcription in experiments using episomal reporter systems Warner 1984, 1986). However, this sequence has been shown subsequently to be dispensable for RNA Pol I transcription in the chromosomal context (Wai et al. 2001).In each cell, Pol I-transcribed rDNA coexists in two different chromatin states (Conconi et al. 1989). In these studies, cells were treated with the DNA cross-linking drug psoralen, and it was shown that actively transcribed and transcriptionally inactive rRNA genes differed substantially in their degree of psoralen incorporation. The different migration behavior of the corresponding restriction fragments in agarose gel electrophoresis led to the terms s-ban...

show abstract

The RNA polymerase I transcription machinery

Cited by 147 publications

References 99 publications

Core promoter recognition complex changes accompany liver development

Core promoter recognition complex changes accompany liver development

Is Ribosome Synthesis Controlled by Pol I Transcription?

Actively transcribed rRNA genes in S. cerevisiae are organized in a specialized chromatin associated with the high-mobility group protein Hmo1 and are largely devoid of histone molecules

Contact Info

Product

Resources

About