The RNA of tobacco etch virus contains poly(A)

Hari, V.; Siegel, Albert; Rozek, Charles E.; Timberlake, William E.

doi:10.1016/0042-6822(79)90159-4

Cited by 93 publications

(36 citation statements)

References 17 publications

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“…This was first demonstrated for CPMV (El Manna & Bruening, 1973) and another comovirus, bean pod mottle virus (Semancik, 1974). Tobacco etch virus (TEV), a potyvirus, is also polyadenylated (Hari et al, 1979). The function is still not known.…”

Section: ' Terminal Structuresmentioning

confidence: 99%

Genome Expression of Plant Positive-strand RNA Viruses

Davies

Hull

1982

Journal of General Virology

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Section: ' Terminal Structuresmentioning

confidence: 99%

Genome Expression of Plant Positive-strand RNA Viruses

Davies

Hull

1982

Journal of General Virology

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“…The potyviral genome has a poly(A) sequence at the 3' end (Hari et al, 1979) and is covalently bound at the 5' end to a protein termed VPg (Hari, 1981;Siaw et al, 1985;Riechmann et al, 1989;Murphy et al, 1990). The tobacco etch potyvirus (TEV) genome nucleotide sequence has been determined (Allison et al, 1986) and contains a single open reading frame that is translated into a large polyprotein, as shown in Figure 1 (Allison et al, 1985).…”

Section: Introductionmentioning

confidence: 99%

Nuclear Transport of Plant Potyviral Proteins

Restrepo¹,

Freed²,

Carrington³

1990

The Plant Cell

138

180

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We have used immunoblotting, immunocytochemical, and gene fusion methods to examine the differential subcellular partitioning of tobacco etch potyvirus proteins that are potentially associated with RNA replication. From the earliest timepoints at which viral proteins could be detected, proteins Nla (49-kilodalton proteinase) and Nlb (58-kilodalton polymerase) were localized primarily in the nucleus, whereas the 71-kilodalton cylindrical inclusion protein was identified in the cytoplasm. The Nla and Nlb coding regions were fused to the 8-glucuronidase (GUS) sequence in a plant expression vector, resulting in synthesis of chimeric proteins in transfected protoplasts and in transgenic plants. In situ localization of GUS activity revealed nuclear localization of the GUS-Nla and GUS-NIb fusion proteins and cytoplasmic localization of nonfused GUS. These results indicate that both Nla and Nlb contain nuclear targeting signals, and that they may serve as useful models for studies of plant cell nuclear transport. A discussion of the general utility of the nuclear transport system described here, as well as the role of nuclear translocation of potyviral proteins, is presented.

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“…The RNA is approximately 10000 nucleotides long, and has a 5'-terminal genome-linked protein (VPg) (Siaw et al, 1985;Riechmann et al, 1989;Murphy et al, 1990) and a 3' poly(A) tail (Hari et al, 1979). It contains one long open reading frame which is translated into a large polyprotein (ranging between 340K and 368K depending on the potyvirus) which is subsequently cleaved into smaller polypeptides.…”

Section: Genome Structure and Organizationmentioning

confidence: 99%