The RNA helicase FRH is an ATP-dependent regulator of CK1a in the circadian clock of Neurospora crassa

Lauinger, Linda; Diernfellner, Axel; Falk, Sebastian; Brunner, Michael

doi:10.1038/ncomms4598

Cited by 46 publications

(64 citation statements)

References 44 publications

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“…Phosphorylated WCC leaves the frq promoter. As FRQ becomes hyperphosphorylated, it loses its ability to interact with CK1 (39) and WCC (17), becoming therefore a weak repressor of WCC (40). In addition, dephosphorylation of WCC, as well as new synthesis of these proteins, provide active WC-1 and WC-2 to reinitiate the cycle.…”

Section: Figmentioning

confidence: 99%

Decoupling circadian clock protein turnover from circadian period determination

Larrondo

Olivares-Yañez

Baker

et al. 2015

Science

142

172

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The mechanistic basis of eukaryotic circadian oscillators in model systems as diverse as Neurospora, Drosophila, and mammalian cells is thought to be a transcription-and-translation–based negative feedback loop, wherein progressive and controlled phosphorylation of one or more negative elements ultimately elicits their own proteasome-mediated degradation, thereby releasing negative feedback and determining circadian period length. The Neurospora crassa circadian negative element FREQUENCY (FRQ) exemplifies such proteins; it is progressively phosphorylated at more than 100 sites, and strains bearing alleles of frq with anomalous phosphorylation display abnormal stability of FRQ that is well correlated with altered periods or apparent arrhythmicity. Unexpectedly, we unveiled normal circadian oscillations that reflect the allelic state of frq but that persist in the absence of typical degradation of FRQ. This manifest uncoupling of negative element turnover from circadian period length determination is not consistent with the consensus eukaryotic circadian model.

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Section: Figmentioning

confidence: 99%

Decoupling circadian clock protein turnover from circadian period determination

Larrondo

Olivares-Yañez

Baker

et al. 2015

Science

142

172

View full text Add to dashboard Cite

show abstract

“…As this theme of dual non-overlapping roles for FRH was emerging, contrasting work based in in-vitro studies asserted that FRH played a more active role in the clock, via its ATPase activity, by stimulating a structural change in FRQ [31]. However subsequent analyses have shown that this ATPase-dependent structural change seems to be specific to the in vitro yeast system used for the experiments and does not occur in Neurospora (personal communication from M. Brunner).…”

Section: New Developments In the Negative Arm Functionmentioning

confidence: 99%

Circadian Oscillators: Around the Transcription–Translation Feedback Loop and on to Output

Hurley

Loros

Dunlap

2016

Trends in Biochemical Sciences

160

125

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From cyanobacteria to mammals, organisms have evolved timing mechanisms to adapt to environmental changes in order to optimize survival and improve fitness. To anticipate these regular daily cycles, many organisms manifest near 24-h cell-autonomous oscillations that are sustained by transcription–translation-based or post-transcriptional negative feedback loops that control a wide range of biological processes. With an eye to identifying emerging common themes among cyanobacterial, fungal and animal clocks, some major recent developments in the understanding of the mechanisms that regulate these oscillators and their output are discussed. These include roles for antisense transcription, intrinsically disordered proteins, codon bias in clock genes, and a more focused discussion of post-transcriptional and translational regulation as a part of both the oscillator and output.

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“…Genome Research 1353 www.genome.org RNA helicases (Sun et al 2011;Cruciat et al 2013;Lauinger et al 2014;Alessi et al 2015).…”

Section: Ddx54 In Genotoxic Stress Responsementioning

confidence: 99%

DDX54 regulates transcriptome dynamics during DNA damage response

et al. 2017

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The cellular response to genotoxic stress is mediated by a well-characterized network of DNA surveillance pathways. The contribution of post-transcriptional gene regulatory networks to the DNA damage response (DDR) has not been extensively studied. Here, we systematically identified RNA-binding proteins differentially interacting with polyadenylated transcripts upon exposure of human breast carcinoma cells to ionizing radiation (IR). Interestingly, more than 260 proteins, including many nucleolar proteins, showed increased binding to poly(A) + RNA in IR-exposed cells. The functional analysis of DDX54, a candidate genotoxic stress responsive RNA helicase, revealed that this protein is an immediate-to-early DDR regulator required for the splicing efficacy of its target IR-induced pre-mRNAs. Upon IR exposure, DDX54 acts by increased interaction with a well-defined class of pre-mRNAs that harbor introns with weak acceptor splice sites, as well as by proteinprotein contacts within components of U2 snRNP and spliceosomal B complex, resulting in lower intron retention and higher processing rates of its target transcripts. Because DDX54 promotes survival after exposure to IR, its expression and/or mutation rate may impact DDR-related pathologies. Our work indicates the relevance of many uncharacterized RBPs potentially involved in the DDR.

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The RNA helicase FRH is an ATP-dependent regulator of CK1a in the circadian clock of Neurospora crassa

Cited by 46 publications

References 44 publications

Decoupling circadian clock protein turnover from circadian period determination

Decoupling circadian clock protein turnover from circadian period determination

Circadian Oscillators: Around the Transcription–Translation Feedback Loop and on to Output

DDX54 regulates transcriptome dynamics during DNA damage response

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