“…As a mechanism of splicing alterations, AR variants are induced via splicing modulation involving various molecules, including U2AF65, ASF/SF2, hnRNPA1, Sam68, Lin28, DDX39, and YB-1. 17,[26][27][28][29][30] In our study, induction of AR variants by TSC was accompanied by phosphorylation of Akt at Thr308, but no YB-1 phosphorylation, suggesting that an Akt-but not YB-1-mediated mechanism is responsible. Consistently, Akt inhibition by LY294002 suppressed expression of AR variants at the protein but not transcript level.…”