2013
DOI: 10.1002/cyto.a.22423
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The RNA/DNA‐binding protein PSF relocates to cell membrane and contributes cells' sensitivity to antitumor drug, doxorubicin

Abstract: Cell surface proteins play an important role in multidrug resistance (MDR). However, the identification involving chemoresistant features for cell surface proteins is a challenge. To identify potential cell membrane markers in hematologic cancer MDR, we used a cell-and antibody-based strategy of subtractive immunization coupled with cell surface comparative screening of leukemia cell lines from sensitive HL60 and resistant HL60/DOX cells. Fifty one antibodies that recognized the cell surface proteins expressed… Show more

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Cited by 9 publications
(10 citation statements)
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“…After washing the beads, proteins were eluted from the beads in a 1x SDS sample buffer and boiled for 10 minutes at 99°C. The precipitates were separated by SDS-PAGE and stained with Coomassie Brilliant Blue as discussed [29].…”
Section: Western Blot Analysismentioning
confidence: 99%
“…After washing the beads, proteins were eluted from the beads in a 1x SDS sample buffer and boiled for 10 minutes at 99°C. The precipitates were separated by SDS-PAGE and stained with Coomassie Brilliant Blue as discussed [29].…”
Section: Western Blot Analysismentioning
confidence: 99%
“…As a result, HeLa cells depleted of APE1 express twofold to fivefold more c‐myc RNA in comparison to control cells . c‐myc mRNA is not the only substrate for APE1 as recent studies have demonstrated that CD44 mRNA, miRNAs, SARS‐corona virus RNA components, FGF2, and FGFR3 mRNAs are also cleaved by APE1 in vitro …”
Section: Involvement Of the Ber Pathway In Rna Metabolismmentioning
confidence: 99%
“…13 c-myc mRNA is not the only substrate for APE1 as recent studies have demonstrated that CD44 mRNA, miR-NAs, SARS-corona virus RNA components, FGF2, and FGFR3 mRNAs are also cleaved by APE1 in vitro. [74][75][76] APE1 has also been shown to play an important role in rRNA quality control. This function was characterized through the identification of an interaction between the APE1 N-terminal region and nucleophosmin (NPM1), a nucleolar protein involved in rRNA processing and ribosome biogenesis 16 (Figure 2).…”
Section: A Role For Ape1 In Rna Repairmentioning
confidence: 99%
“…Data investigating the effects of llme23 overexpression and depletion is more limited than for HOTAIR and SPRY4-IT1, however, Llme23-depleted YUSAC cells displayed a significant decrease in their ability to form colonies in soft-agar and most significantly, these same cells displayed ~75% decrease in tumour volume at day 38 post-injection into nude mice (58). taking into consideration the emerging literature around PSf and cancer (63,64), it will be extremely interesting to observe the delineation of Llme23 function in the literature, which to date is restricted to the article discussed here.…”
Section: Spry4-it1mentioning
confidence: 99%