The RNA Content of Fungal Extracellular Vesicles: At the “Cutting-Edge” of Pathophysiology Regulation

Bitencourt, Tamires Aparecida; Pessoni, André Moreira; Oliveira, Bianca T. M.; Alves, Lysangela Ronalte; Almeida, Fausto

doi:10.3390/cells11142184

Cited by 6 publications

(13 citation statements)

References 64 publications

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“…In fungi, the extracellular transcriptome has been mostly studied through the analysis of RNA molecules associated with EVs (Bitencourt et al 2022b). Several papers reported the presence of small RNAs as well as polyA+ RNAs associated with fungal EV extracts (Bitencourt et al 2022b).…”

Section: Discussionmentioning

confidence: 99%

“…Numerous studies have documented the presence of RNA molecules associated with fungal EVs, including in Cryptococcus (Peres Da Silva et al 2015;Peres Da Silva et al 2018;Liu et al 2020;Reis et al 2021;Bitencourt et al 2022b). Nevertheless, the methods of growth, EV purification, and RNA sequencing employed in these studies vary significantly.…”

Section: Some Rnas Are Ev-associated In Cryptococcusmentioning

confidence: 99%

“…Because a large diversity of RNAs (siRNAs, tRNA halves, lncRNAs, mRNAs, snRNAs, snoRNAs…) have been shown to be associated with EVs in different organisms including fungi (Rizzo et al 2021a;Bitencourt et al 2022b), we first constructed a sequencing library using a small RNA library preparation kit on total RNA extracted from EVs and previously submitted to decapping treatment (Wallace et al 2020), yet no size selection step was implemented at this step. We reasoned that this approach should provide us with a comprehensive view of the Cryptococcus EV transcriptome since it should generate small RNA reads as well as, albeit to a lesser extent, longer reads up to 100 nt corresponding to mRNAs or lncRNAs fragments from the same samples.…”

Section: Small Rnas Associated With Evsmentioning

confidence: 99%

“…2021a). In fact, despite a few published studies (Bitencourt et al . 2022b), there is no real consensus on the structure of the fungal EVs transcriptome.…”

mentioning

confidence: 99%

“…Therefore, it is difficult to estimate the share of simple contaminants within the pool of RNA species identified. Thus, although RNAs associated with fungal EVs have been reported in many species (Bitencourt et al . 2022b), the fungal EV transcriptome remains poorly described.…”

mentioning

confidence: 99%

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TheCryptococcusextracellular vesicle transcriptome

Taheraly,

Maufrais,

Moyrand

et al. 2023

Preprint

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Extracellular vesicles (EVs) are now recognized as key players in the biology of numerous organisms, including pathogenic fungi. However, studying EVs in these organisms remains challenging. The recent implementation of new protocols to purify EVs in the pathogenic yeastCryptococcus neoformanshas resulted in a more detailed description of their structure and protein composition. Although a few publications describing RNA molecules associated with EVs have already been published, we reasoned that these new protocols would be beneficial for gaining a deeper understanding of the EV transcriptome. We thus purified EVs and confirmed that some RNAs were associated with these EV extracts. Iodixanol gradient analyses also revealed that these RNAs co-sedimented with EVs. We then sequenced these RNAs in parallel with RNAs extracted from the very cells producing these EVs using different types of sequencing libraries. Our data confirm the presence of siRNAs and tRFs associated with EVs, some of which are enriched. We also identified some snoRNAs, which inCryptococcus are mostly borne by coding gene or lncRNA introns.

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Section: Discussionmentioning

confidence: 99%

Section: Some Rnas Are Ev-associated In Cryptococcusmentioning

confidence: 99%

Section: Small Rnas Associated With Evsmentioning

confidence: 99%

“…2021a). In fact, despite a few published studies (Bitencourt et al . 2022b), there is no real consensus on the structure of the fungal EVs transcriptome.…”

mentioning

confidence: 99%

mentioning

confidence: 99%

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TheCryptococcusextracellular vesicle transcriptome

Taheraly,

Maufrais,

Moyrand

et al. 2023

Preprint

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Yeast strains isolated from fermented beverage produce extracellular vesicles with anti-inflammatory effects

Nenciarini,

Amoriello,

Bacci

et al. 2024

Sci Rep

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Extracellular vesicles (EVs) are lipid-bilayered particles, containing various biomolecules, including nucleic acids, lipids, and proteins, released by cells from all the domains of life and performing multiple communication functions. Evidence suggests that the interaction between host immune cells and fungal EVs induces modulation of the immune system. Most of the studies on fungal EVs have been conducted in the context of fungal infections; therefore, there is a knowledge gap in what concerns the production of EVs by yeasts in other contexts rather than infection and that may affect human health. In this work, we characterized EVs obtained by Saccharomyces cerevisiae and Pichia fermentans strains isolated from a fermented milk product with probiotic properties. The immunomodulation abilities of EVs produced by these strains have been studied in vitro through immune assays after internalization from human monocyte-derived dendritic cells. Results showed a significant reduction in antigen presentation activity of dendritic cells treated with the fermented milk EVs. The small RNA fraction of EVs contained mainly yeast mRNA sequences, with a few molecular functions enriched in strains of two different species isolated from the fermented milk. Our results suggest that one of the mechanisms behind the anti-inflammatory properties of probiotic foods could be mediated by the interactions of human immune cells with yeast EVs.

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Characterization and immunomodulatory potential of extracellular vesicles from non-pathogenic yeast strains

Nenciarini,

Amoriello,

Bacci

et al. 2023

Preprint

View full text Add to dashboard Cite

Extracellular vesicles (EVs) are lipid-bilayered particles, containing various biomolecules, including nucleic acids, lipids, and proteins, released by cells from all the domains of life and performing multiple communication functions. Evidence suggests that the interaction between host immune cells and fungal EVs induces modulation of the immune system. Most of the studies on fungal EVs have been conducted in the context of fungal infections; therefore, there is still a knowledge gap regarding EVs produced by non-pathogenic yeasts. In this work, we characterized EVs obtained by Saccharomyces cerevisiae and Pichia fermentans strains isolated from a fermented milk product with probiotic properties. The immunomodulation abilities of EVs produced by these strains have been studied in vitro through immune assays after internalization from human monocyte-derived dendritic cells. Results showed a significant reduction in antigen presentation activity of dendritic cells treated with the fermented milk EVs. The small RNA fraction of EVs contained mainly yeast mRNA sequences, with a few molecular functions enriched in strains of two different species isolated from the fermented milk. Our results suggest that one of the mechanisms behind the anti-inflammatory properties of probiotic foods could be mediated by the interactions of human immune cells with yeast EVs.

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The RNA Content of Fungal Extracellular Vesicles: At the “Cutting-Edge” of Pathophysiology Regulation

Cited by 6 publications

References 64 publications

TheCryptococcusextracellular vesicle transcriptome

TheCryptococcusextracellular vesicle transcriptome

Yeast strains isolated from fermented beverage produce extracellular vesicles with anti-inflammatory effects

Characterization and immunomodulatory potential of extracellular vesicles from non-pathogenic yeast strains

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