The RNA-binding proteins CSP41a and CSP41b may regulate transcription and translation of chloroplast-encoded RNAs in Arabidopsis

Bollenbach, Thomas; Sharwood, Robert E.; Gutierrez, Ryan; Lerbs-Mache, Silva; Stern, David B.

doi:10.1007/s11103-008-9436-z

Cited by 66 publications

(75 citation statements)

References 41 publications

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“…Previous studies have provided strong in vivo and in vitro evidence that mRNA-binding proteins translocate to ribosomes and associate with mRNA to stabilize the mRNA, facilitating its translation or degradation [28,29]. Park et al [30] found that the RNA encoding a small RNA-binding protein was involved in abiotic stress signaling.…”

Section: Discussionmentioning

confidence: 99%

Phosphoproteins regulated by heat stress in rice leaves

Chen

Zhang

et al. 2011

Proteome Sci

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BackgroundHigh temperature is a critical abiotic stress that reduces crop yield and quality. Rice (Oryza sativa L.) plants remodel their proteomes in response to high temperature stress. Moreover, phosphorylation is the most common form of protein post-translational modification (PTM). However, the differential expression of phosphoproteins induced by heat in rice remains unexplored.MethodsPhosphoprotein in the leaves of rice under heat stress were displayed using two-dimensional electrophoresis (2-DE) and Pro-Q Diamond dye. Differentially expressed phosphoproteins were identified by MALDI-TOF-TOF-MS/MS and confirmed by Western blotting.ResultsTen heat-phosphoproteins were identified from twelve protein spots, including ribulose bisphos-phate carboxylase large chain, 2-Cys peroxiredoxin BAS1, putative mRNA binding protein, Os01g0791600 protein, OSJNBa0076N16.12 protein, putative H(+)-transporting ATP synthase, ATP synthase subunit beta and three putative uncharacterized proteins. The identification of ATP synthase subunit beta was further validated by Western-blotting. Four phosphorylation site predictors were also used to predict the phosphorylation sites and the specific kinases for these 10 phosphoproteins.ConclusionHeat stress induced the dephosphorylation of RuBisCo and the phosphorylation of ATP-β, which decreased the activities of RuBisCo and ATP synthase. The observed dephosphorylation of the mRNA binding protein and 2-Cys peroxiredoxin may be involved in the transduction of heat-stress signaling, but the functional importance of other phosphoproteins, such as H+-ATPase, remains unknown.

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Section: Discussionmentioning

confidence: 99%

Phosphoproteins regulated by heat stress in rice leaves

Chen

Zhang

et al. 2011

Proteome Sci

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“…The RNase treatment did result in additional loss of proteins, as compared with the co-IP effect alone, as we identified in this fraction 473 proteins compared with 546 in the co-IP sample without RNase treatment (Supplemental Table S6). The 10 most abundant proteins lost after RNase treatment were PSRP-2, known to be associated with 30S ribosome particles (Yamaguchi and Subramanian, 2003), two homologs of Rap38/CSP41B-2 (Yang et al, 1996;Bollenbach et al, 2009), 50S ribosomal protein L9, an RRM protein, iron superoxide dismutase (Fe-SOD or FSD3), an agglutinin domain protein, RNA-binding protein cp33 (RRM), DNA polymerase I, and another RRM-containing protein (Supplemental Table S6). …”

Section: Coimmunoprecipitation With Anti-why1 and Rnase Treatmentmentioning

confidence: 99%

Nucleoid-Enriched Proteomes in Developing Plastids and Chloroplasts from Maize Leaves: A New Conceptual Framework for Nucleoid Functions

et al. 2011

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Plastids contain multiple copies of the plastid chromosome, folded together with proteins and RNA into nucleoids. The degree to which components of the plastid gene expression and protein biogenesis machineries are nucleoid associated, and the factors involved in plastid DNA organization, repair, and replication, are poorly understood. To provide a conceptual framework for nucleoid function, we characterized the proteomes of highly enriched nucleoid fractions of proplastids and mature chloroplasts isolated from the maize (Zea mays) leaf base and tip, respectively, using mass spectrometry. Quantitative comparisons with proteomes of unfractionated proplastids and chloroplasts facilitated the determination of nucleoid-enriched proteins. This nucleoid-enriched proteome included proteins involved in DNA replication, organization, and repair as well as transcription, mRNA processing, splicing, and editing. Many proteins of unknown function, including pentatricopeptide repeat (PPR), tetratricopeptide repeat (TPR), DnaJ, and mitochondrial transcription factor (mTERF) domain proteins, were identified. Strikingly, 70S ribosome and ribosome assembly factors were strongly overrepresented in nucleoid fractions, but protein chaperones were not. Our analysis strongly suggests that mRNA processing, splicing, and editing, as well as ribosome assembly, take place in association with the nucleoid, suggesting that these processes occur cotranscriptionally. The plastid developmental state did not dramatically change the nucleoid-enriched proteome but did quantitatively shift the predominating function from RNA metabolism in undeveloped plastids to translation and homeostasis in chloroplasts. This study extends the known maize plastid proteome by hundreds of proteins, including more than 40 PPR and mTERF domain proteins, and provides a resource for targeted studies on plastid gene expression. Details of protein identification and annotation are provided in the Plant Proteome Database.

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“…These proteins play a role in the expression of plastid genes and may be involved in the biogenesis of plastidial ribosomes (Beligni and Mayfield, 2008;Bollenbach et al, 2009). Several proteins involved in chloroplast division, namely, FtsZ1, FtsZ2, Arc5, and Cpn60B (Gao et al, 2003), are downregulated in the C 4 species.…”

Section: Chloroplast Biogenesis and Maintenance Is Altered In C 4 Spementioning

confidence: 99%

Evolution of C4 Photosynthesis in the GenusFlaveria: How Many and Which Genes Does It Take to Make C4?

et al. 2011

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Selective pressure exerted by a massive decline in atmospheric CO 2 levels 55 to 40 million years ago promoted the evolution of a novel, highly efficient mode of photosynthetic carbon assimilation known as C 4 photosynthesis. C 4 species have concurrently evolved multiple times in a broad range of plant families, and this multiple and parallel evolution of the complex C 4 trait indicates a common underlying evolutionary mechanism that might be elucidated by comparative analyses of related C 3 and C 4 species. Here, we use mRNA-Seq analysis of five species within the genus Flaveria, ranging from C 3 to C 3 -C 4 intermediate to C 4 species, to quantify the differences in the transcriptomes of closely related plant species with varying degrees of C 4 -associated characteristics. Single gene analysis defines the C 4 cycle enzymes and transporters more precisely and provides new candidates for yet unknown functions as well as identifies C 4 associated pathways. Molecular evidence for a photorespiratory CO 2 pump prior to the establishment of the C 4 cycle-based CO 2 pump is provided. Cluster analysis defines the upper limit of C 4 -related gene expression changes in mature leaves of Flaveria as 3582 alterations.

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The RNA-binding proteins CSP41a and CSP41b may regulate transcription and translation of chloroplast-encoded RNAs in Arabidopsis

Cited by 66 publications

References 41 publications

Phosphoproteins regulated by heat stress in rice leaves

Phosphoproteins regulated by heat stress in rice leaves

Nucleoid-Enriched Proteomes in Developing Plastids and Chloroplasts from Maize Leaves: A New Conceptual Framework for Nucleoid Functions

Evolution of C4 Photosynthesis in the GenusFlaveria: How Many and Which Genes Does It Take to Make C4?

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