The RNA binding protein RBPMS is a selective marker of ganglion cells in the mammalian retina

Rodriguez, Allen; Müller, Luis Pérez de Sevilla; Brecha, Nicholas C.

doi:10.1002/cne.23521

Cited by 400 publications

(464 citation statements)

References 124 publications

(299 reference statements)

Supporting

Mentioning

419

Contrasting

Order By: Relevance

“…We used RNA-binding protein with multiple splicing (RBPMS) as a selective marker of mice RGCs (34). The eyes were enucleated, and the posterior segments were immediately dissected and fixed with 4% paraformaldehyde in PBS for 1 hour.…”

Section: Methodsmentioning

confidence: 99%

GDF15 is elevated in mice following retinal ganglion cell death and in glaucoma patients

Ban¹,

Siegfried²,

Lin³

et al. 2017

JCI Insight

View full text Add to dashboard Cite

Section: Methodsmentioning

confidence: 99%

GDF15 is elevated in mice following retinal ganglion cell death and in glaucoma patients

Ban¹,

Siegfried²,

Lin³

et al. 2017

JCI Insight

View full text Add to dashboard Cite

“…5A, Right). Colocalization experiments comparing X-Gal labeling and immunohistochemistry with a ganglion-cell marker, retinal binding protein with multiple splicing (RBPMS) (31,32) (Fig. 5B), suggested that the X-Galpositive neurons constitute a small subset of ganglion cells rather than displaced amacrine cells.…”

Section: Significancementioning

confidence: 99%

Neuropsin (OPN5)-mediated photoentrainment of local circadian oscillators in mammalian retina and cornea

Buhr

Yue

Ren

et al. 2015

Proc. Natl. Acad. Sci. U.S.A.

125

181

View full text Add to dashboard Cite

The molecular circadian clocks in the mammalian retina are locally synchronized by environmental light cycles independent of the suprachiasmatic nuclei (SCN) in the brain. Unexpectedly, this entrainment does not require rods, cones, or melanopsin (OPN4), possibly suggesting the involvement of another retinal photopigment. Here, we show that the ex vivo mouse retinal rhythm is most sensitive to short-wavelength light but that this photoentrainment requires neither the short-wavelength-sensitive cone pigment [S-pigment or cone opsin (OPN1SW)] nor encephalopsin (OPN3). However, retinas lacking neuropsin (OPN5) fail to photoentrain, even though other visual functions appear largely normal. Initial evidence suggests that OPN5 is expressed in select retinal ganglion cells. Remarkably, the mouse corneal circadian rhythm is also photoentrainable ex vivo, and this photoentrainment likewise requires OPN5. Our findings reveal a light-sensing function for mammalian OPN5, until now an orphan opsin.

show abstract

“…Additional Foxg1 +/+ and Foxg1 +/Cre mice were used for counting cells in the ganglion cell layer (GCL) following published protocols (Damiani et al, 2012, Rodriguez et al, 2014. Briefly, paraformaldheyde fixed retinas (n=3 per strain, each from a different animal) were isolated from eye cups, the vitreous removed and 4 partial cuts made to delimitate the dorsal, nasal, ventral and temporal quadrants.…”

Section: Counts Of Cells In the Ganglion Cell Layer (Gcl)mentioning

confidence: 99%

Visual impairment in FOXG1-mutated individuals and mice

et al. 2016

View full text Add to dashboard Cite

The Forkead Box G1 (FOXG1) gene encodes for a DNA−binding transcription factor, essential for the development of the telencephalon in mammalian forebrain. Mutations in FOXG1 have been reported to be involved in the onset of Rett Syndrome, for which sequence alterations of MECP2 and CDKL5 are known. While visual alterations are not classical hallmarks of Rett syndrome, an increasing body of evidence shows visual impairment in patients and in MeCP2 and CDKL5 animal models. Herein we focused on the functional role of FOXG1 in the visual system of animal models (Foxg1+/Cre mice) and of a cohort of patients carrying FOXG1 mutations or deletions. Visual physiology of Foxg1+/Cre mice was assessed by visually evoked potentials, which revealed a significant reduction in response amplitude and visual acuity with respect to wild-type littermates. Morphological investigation showed abnormalities in the organization of excitatory/inhibitory circuits in the visual cortex. No alterations were observed in retinal structure. By examining a cohort of FOXG1-mutated patients by means of a panel of neuro-ophthalmological evaluations, we found that all of them exhibited visual alterations compatible with high level visual dysfunctions. In conclusion our data show that Foxg1 haploinsufficiency results in an impairment of mouse and patient visual function. Response to Reviewers: Pisa February 27th 2016Dear Editor, We would like to thank the referees for the thoughtful critiques on our manuscript and for the opportunity to revise our work. Please find enclosed a revised version of our manuscript (NSC-15-1232) entitled "Visual impairment in FOXG1-mutated patients and mice" by Boggio et al. As requested, we performed additional experiments on retina and visual cortex in order to more quantitavely prove the structure specific alterations and strengthen our conclusions. We included below a detailed response to the reviewer comments (in italics). References have been updated and added as suggested by referees. Sincerely, Tommaso PizzorussoReviewer #1:Major concerns 1.The description of the morphology of the five classes of retinal neurons would benefit from a quantitative approach amenable to statistical comparisons, similar to that performed for cortical GABAergic neurons in Fig. 4.We performed the requested quantitative analysis that is now shown in fig. 4. The analysis was focussed on cells in the ganglion cell layer because this layer is known to be affected in Foxg1 null mice. No significant change in the overall number of cells, and in ganglion cells identified using the RBPMS cell type-specific antibody, was present in Foxg1 heterozygous mice 2.There are no explicit statements regarding blinding of investigators to mouse genotypes during data acquisition and analyses, the use of Power Analysis to determine sample sizes, and predetermined criteria to exclude data sets. These are all critical issues that need to be performed and explicitly reported as recommended (Landis et al. Nature 2012).We included in the methods the fol...

show abstract

The RNA binding protein RBPMS is a selective marker of ganglion cells in the mammalian retina

Cited by 400 publications

References 124 publications

GDF15 is elevated in mice following retinal ganglion cell death and in glaucoma patients

GDF15 is elevated in mice following retinal ganglion cell death and in glaucoma patients

Neuropsin (OPN5)-mediated photoentrainment of local circadian oscillators in mammalian retina and cornea

Visual impairment in FOXG1-mutated individuals and mice

Contact Info

Product

Resources

About