The RNA-Binding Protein NONO Coordinates Hepatic Adaptation to Feeding

Benegiamo, Giorgia; Mure, Ludovic S.; Erikson, Galina; Le, Hiep D.; Moriggi, Ermanno; Brown, Steven A.; Panda, Satchidananda

doi:10.1016/j.cmet.2017.12.010

Cited by 83 publications

(69 citation statements)

References 83 publications

(97 reference statements)

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“…Accumulating evidence suggests that altogether peripheral clocks play a critical role in regulating glucose and lipid homeostasis . Of note, mutations in the essential clock genes BMAL1 and CLOCK cause various metabolic disorders, while perturbations of metabolic pathways in mice, fed with a high‐fat diet, dampen the amplitude of circadian oscillations and lengthen their period .…”

Section: Body Metabolism Is Orchestrated By Circadian Clocks In Rodenmentioning

confidence: 99%

“…54 Accumulating evidence suggests that altogether peripheral clocks play a critical role in regulating glucose and lipid homeostasis. 50,53,[55][56][57] Of note, mutations in the essential clock genes BMAL1 and CLOCK 46,58 cause various metabolic disorders, while perturbations of metabolic pathways in mice, fed with a high-fat diet, dampen the amplitude of circadian oscillations and lengthen their period. 59 Moreover, the expression of circadian output genes dramatically changes in these animals, partly due to the inhibition of CLOCK-BMAL1 recruitment to chromatin and the activation of PPARγ.…”

Section: Body Metabolism Is Orchestrated By Circadian Clocks In Rodmentioning

confidence: 99%

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Time zones of pancreatic islet metabolism

Petrenko

Philippe

Dibner

2018

Diabetes Obesity Metabolism

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Most living beings possess an intrinsic system of circadian oscillators, allowing anticipation of the Earth's rotation around its own axis. The mammalian circadian timing system orchestrates nearly all aspects of physiology and behaviour. Together with systemic signals originating from the central clock that resides in the hypothalamic suprachiasmatic nucleus, peripheral oscillators orchestrate tissue-specific fluctuations in gene transcription and translation, and posttranslational modifications, driving overt rhythms in physiology and behaviour. There is accumulating evidence of a reciprocal connection between the circadian oscillator and most aspects of physiology and metabolism, in particular as the circadian system plays a critical role in orchestrating body glucose homeostasis. Recent reports imply that circadian clocks operative in the endocrine pancreas regulate insulin secretion, and that islet clock perturbation in rodents leads to the development of overt type 2 diabetes. While whole islet clocks have been extensively studied during the last years, the heterogeneity of islet cell oscillators and the interplay between α- and β-cellular clocks for orchestrating glucagon and insulin secretion have only recently gained attention. Here, we review recent findings on the molecular makeup of the circadian clocks operative in pancreatic islet cells in rodents and in humans, and focus on the physiologically relevant synchronizers that are resetting these time-keepers. Moreover, the implication of islet clock functional outputs in the temporal coordination of metabolism in health and disease will be highlighted.

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Section: Body Metabolism Is Orchestrated By Circadian Clocks In Rodenmentioning

confidence: 99%

Section: Body Metabolism Is Orchestrated By Circadian Clocks In Rodmentioning

confidence: 99%

Time zones of pancreatic islet metabolism

Petrenko

Philippe

Dibner

2018

Diabetes Obesity Metabolism

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“…Indeed, muscle-specific loss of BMAL1 directly leads to metabolic inefficiency, impairs muscle triglyceride biosynthesis, and causes accumulation of bioactive lipids and amino acids in mice. 128 Advanced approaches for circadian metabolomic and lipidomic studies reveal that a large number of metabolites are oscillating around-the-clock in rodents and in human tissues, plasma and saliva. 122 Liver-specific Bmal1KO leads to reduced lipid accumulation in hepatic cells via increased mRNA methylation, in particular of PPARα.…”

Section: Of Mammalian Physiology and Metabolismmentioning

confidence: 99%

“…127 Additionally, the RNA-binding protein NONO situated in liver cell nuclei couples the rhythmic expression of metabolic genes with nutrient levels, with NONO-deficient mice exhibiting impaired glucose tolerance as well as lower hepatic glycogen and lipid content. 128 Advanced approaches for circadian metabolomic and lipidomic studies reveal that a large number of metabolites are oscillating around-the-clock in rodents and in human tissues, plasma and saliva. 40,53,[129][130][131][132][133][134][135] Integrative metabolomic profiling of 8 mouse tissues, conducted around the clock upon physiological conditions or high-fat diet, further exemplifies this tight temporal orchestration of whole-body metabolism by nutrient intake.…”

Section: Dibnermentioning

confidence: 99%

The importance of being rhythmic: Living in harmony with your body clocks

Dibner

2019

Acta Physiologica

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OSCILLATORSThe time-keeping system, dubbed "circadian" from Latin circa diem (about a day), allows light-sensitive beings, including humans, to coordinate their physiology and behaviour to the daily changes in geophysical time (Figure 1). The mammalian network of body clocks is organized in a strictly hierarchical manner. A master oscillator, residing in the paired suprachiasmatic nuclei (SCN) of the hypothalamus ( Figure 1A), synchronizes a myriad of peripheral oscillators situated in each organ ( Figure 1B) on a daily basis. In turn, rhythmicity in the SCN is entrained by external timecues or Zeitgebers (German term for time givers). Daily changes in light intensity, detected by classical photoreceptors and intrinsically light-sensitive retinal ganglion cells expressing AbstractCircadian rhythms have developed in all light-sensitive organisms, including humans, as a fundamental anticipatory mechanism that enables proactive adaptation to environmental changes. The circadian system is organized in a highly hierarchical manner, with clocks operative in most cells of the body ensuring the temporal coordination of physiological processes. Circadian misalignment, stemming from modern life style, draws increasing attention due to its tight association with the development of metabolic, cardiovascular, inflammatory and mental diseases as well as cancer.This review highlights recent findings emphasizing the role of the circadian system in the temporal orchestration of physiology, with a particular focus on implications of circadian misalignment in human pathologies. K E Y W O R D Scircadian clock, circadian misalignment, continuous recording of circadian bioluminescence, human physiology How to cite this article: Dibner C. The importance of being rhythmic: Living in harmony with your body clocks. Acta Physiol. 2020;228:e13281. https ://doi.

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“…Cells were treated with or without rosiglitazone for 6 h and lysed in Pro-Prep Protein Extraction Solution (Intron Biotechnology, Seoul, Korea) supplemented with 0.5 U RNaseOut (Thermo Fisher Scientific). Endogenous RNA-HuR complexes were immunoprecipitated under native conditions as previously described by Benegiamo et al (28) with modifications. Briefly, total cell lysates were incubated with Protein G Sepharose 4 Fast Flow resin (GE Healthcare) and normal mouse IgG or anti-HuR antibodies at 4°C.…”

Section: Rna Immunoprecipitationmentioning

confidence: 99%

Rosiglitazone‐dependent dissociation of HuR from PPAR‐γ regulates adiponectin expression at the posttranscriptional level

Hwang

Lee²,

Hur³

et al. 2019

FASEB j.

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Peroxisome proliferator–activated receptor (PPAR)‐γ has been implicated as a key player in the regulation of adiponectin levels via both transcriptional and posttranscriptional mechanisms. Herein, we show that PPAR‐γ interacts with human antigen R (HuR) and that the PPAR‐γ–HuR complex dissociates following activation of PPAR‐γ by rosiglitazone, a specific ligand of PPAR‐γ. This rosiglitazone‐dependent dissociation of HuR from PPAR‐γ leads to nucleocytoplasmic shuttling of HuR and its binding to the 3′‐UTR of adiponectin mRNA. PPAR‐γ with H321A and H447A double mutation (PPAR‐γH321/447A), a mutant lacking ligand‐binding activity, impaired HuR dissociation from the PPAR‐γ–HuR complex, resulting in reduced nucleocytoplasmic shuttling, even in the presence of rosiglitazone. Consequently, rosiglitazone up‐regulated adiponectin levels by modulating the stability of adiponectin mRNA, whereas these effects were abolished by HuR ablation or blocked in cells expressing the PPAR‐γH321/447A mutant, indicating that the interaction of PPAR‐γ and HuR is a critical event during adiponectin expression. Taken together, the findings demonstrate a novel mechanism for regulating adiponectin expression at the posttranscriptional level and suggest that ligand‐mediated activation of PPAR‐γ to interfere with interaction of HuR could offer a therapeutic strategy for inflammation‐associated diseases that involve decreased adiponectin mRNA stability.—Hwang, J. S., Lee, W. J., Hur, J., Lee, H. G., Kim, E., Lee, G. H., Choi, M.‐J., Lim, D.‐S., Paek, K. S., Seo, H. G. Rosiglitazone‐dependent dissociation of HuR from PPAR‐γ regulates adiponectin expression at the posttranscriptional level. FASEB J. 33, 7707–7720 (2019). http://www.fasebj.org

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The RNA-Binding Protein NONO Coordinates Hepatic Adaptation to Feeding

Cited by 83 publications

References 83 publications

Time zones of pancreatic islet metabolism

Time zones of pancreatic islet metabolism

The importance of being rhythmic: Living in harmony with your body clocks

Rosiglitazone‐dependent dissociation of HuR from PPAR‐γ regulates adiponectin expression at the posttranscriptional level

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