The RNA-binding protein Mex3b has a fine-tuning system for mRNA regulation in early<i>Xenopus</i>development

Takada, Hitomi; Kawana, Toshio; Ito, Yuzuru; Kikuno, Reiko; Mamada, Hiroshi; Araki, Toshiyuki; Koga, Hisashi; Asashima, Makoto; Taira, Masanori

doi:10.1242/dev.029165

Cited by 19 publications

(25 citation statements)

References 42 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…As frequently observed for RNA-binding proteins (Takada et al 2009;Cho et al 2012;Yu et al 2014), changes in mRNA levels caused by TRIM71 on its targets were mod-est. Nonetheless, targets may be very dose-sensitive, with small changes in abundance having large effects.…”

Section: Trim71 Modulates Alternative Splicing By Repressing Mbnl1mentioning

confidence: 67%

The RNA hairpin binder TRIM71 modulates alternative splicing by repressing MBNL1

et al. 2019

View full text Add to dashboard Cite

TRIM71/LIN-41, a phylogenetically conserved regulator of development, controls stem cell fates. Mammalian TRIM71 exhibits both RNA-binding and protein ubiquitylation activities, but the functional contribution of either activity and relevant primary targets remain poorly understood. Here, we demonstrate that TRIM71 shapes the transcriptome of mouse embryonic stem cells (mESCs) predominantly through its RNA-binding activity. We reveal that TRIM71 binds targets through 3 ′ untranslated region (UTR) hairpin motifs and that it acts predominantly by target degradation. TRIM71 mutations implicated in etiogenesis of human congenital hydrocephalus impair target silencing. We identify a set of primary targets consistently regulated in various human and mouse cell lines, including MBNL1 (Muscleblind-like protein 1). MBNL1 promotes cell differentiation through regulation of alternative splicing, and we demonstrate that TRIM71 promotes embryonic splicing patterns through MBNL1 repression. Hence, repression of MBNL1-dependent alternative splicing may contribute to TRIM71's function in regulating stem cell fates.

show abstract

Section: Trim71 Modulates Alternative Splicing By Repressing Mbnl1mentioning

confidence: 67%

The RNA hairpin binder TRIM71 modulates alternative splicing by repressing MBNL1

et al. 2019

View full text Add to dashboard Cite

show abstract

“…For gene expression to be established at an appropriate level, some of these regulatory processes must be under rapid and precise control. Because transcriptional regulation is not sufficient to determine the level of gene expression and its duration, post-transcriptional regulation is necessary for finely tuned gene expression 15 . While interest in post-transcriptional regulation has increased recently with the explosive discoveries of large numbers of non-coding RNAs such as microRNAs, post-transcriptional processes still depend largely on the functions of RNA-binding proteins (RBPs) 16 .…”

Section: Introductionmentioning

confidence: 99%

Vimentin interacts with the 5′-untranslated region of mouse mu opioid receptor (MOR) and is required for post-transcriptional regulation

et al. 2013

View full text Add to dashboard Cite

The opioid receptors are among the most highly studied members of the superfamily of G-protein coupled receptors. Morphine and endogenous mu opioid peptides exert their pharmacological actions mainly through the mu opioid receptor (MOR). Expression of opioid receptor proteins is controlled by extensive transcriptional and post-transcriptional processing. Previously, the 5′-untranslated region (UTR) of the mouse MOR was found to be important for post-transcriptional regulation of the MOR gene in neuronal cells. Here, we demonstrate for the first time the role of vimentin as a post-transcriptional repressor in MOR gene regulation. To identify potential regulators of the mouse MOR gene, we performed affinity column chromatography using 5′-UTR-specific RNA oligonucleotides using neuroblastoma NS20Y cells. Chromatography was followed by two-dimensional gel electrophoresis and MALDI-TOF mass spectrometry. We identified an intermediate filament protein, vimentin, which bound specifically to the region between -175 and -150 (175–150) of the MOR 5′-UTR. Binding was confirmed by western blot analysis and RNA supershift assay. Furthermore, a cotransfection study demonstrated that the presence of vimentin resulted in reduced expression of the mouse MOR. Our data suggest that vimentin functions as a repressor of MOR translation, dependent on 175–150 of the MOR 5′-UTR.

show abstract

“…Human ortologs were characterized as related to RNA-binding phosphoproteins that shuttle between the nucleus and the cytoplasm and, according to their different final sites in the cytoplasm, may participate in mRNA storage, regulation of translation or degradation [81]. Furthermore, due to the ability to directly interact with the protein Argonaute, they are thought to be involved in RNA interference and miRNA biosynthesis pathways [82]. This last finding is corroborated by the up regulation of another gene with miRNA processing activity, an exportin-like protein, Smp_124820.1— chromosome region maintenance protein .…”

Section: Discussionmentioning

confidence: 99%

Comparative sequence analysis reveals regulation of genes in developing schistosomula of Schistosoma mansoni exposed to host portal serum

et al. 2017

View full text Add to dashboard Cite

Once inside a vertebrate host after infection, individual schistosomula of the parasite Schistosoma mansoni find a new and complex environment, which requires quick adjustments for survival, such as those that allow it to avoid the innate immune response of the host. Thus, it is very important for the parasite to remain within the skin after entering the host for a period of about 3 days, at which time it can then reach the venous system, migrate to the lungs and, by the end of eighth day post-infection, it reach the portal venous system, while undergoing minimal changes in morphology. However, after just a few days in the portal blood system, the parasite experiences an extraordinary increase in biomass and significant morphological alterations. Therefore, determining the constituents of the portal venous system that may trigger these changes that causes the parasite to consolidate its development inside the vertebrate host, thus causing the disease schistosomiasis, is essential. The present work simulated the conditions found in the portal venous system of the vertebrate host by exposing schistosomula of S. mansoni to in vitro culture in the presence of portal serum of the hamster, Mesocricetus auratus. Two different incubation periods were evaluated, one of 3 hours and one of 12 hours. These time periods were used to mimic the early contact of the parasite with portal serum during the course of natural infection. As a control, parasites were incubated in presence of hamster peripheral serum, in order to compare gene expression signatures between the two conditions. The mRNA obtained from parasites cultured under both conditions were submitted to a whole transcriptome library preparation and sequenced with a next generation platform. On average, nearly 15 million reads were produced per sample and, for the purpose of gene expression quantification, only reads mapped to one location of the transcriptome were considered. After statistical analysis, we found 103 genes differentially expressed by schistosomula cultured for 3 hours and 12 hours in the presence of hamster portal serum. After the subtraction of a second list of genes, also differentially expressed between schistosomula cultured for 3 hours and 12 hours in presence of peripheral serum, a set of 58 genes was finally established. This pattern was further validated for a subset of 17 genes, by measuring gene expression through quantitative real time polymerase chain reaction (qPCR). Processes that were activated by the portal serum stimulus include response to stress, membrane transport, protein synthesis and folding/degradation, signaling, cytoskeleton arrangement, cell adhesion and nucleotide synthesis. Additionally, a smaller number of genes down-regulated under the same condition act on cholinergic signaling, inorganic cation and organic anion membrane transport, cell adhesion and cytoskeleton arrangement. Considering the role of these genes in triggering processes that allow the parasite to quickly adapt, escape the immune response of the host and st...

show abstract

The RNA-binding protein Mex3b has a fine-tuning system for mRNA regulation in earlyXenopusdevelopment

Cited by 19 publications

References 42 publications

The RNA hairpin binder TRIM71 modulates alternative splicing by repressing MBNL1

The RNA hairpin binder TRIM71 modulates alternative splicing by repressing MBNL1

Vimentin interacts with the 5′-untranslated region of mouse mu opioid receptor (MOR) and is required for post-transcriptional regulation

Comparative sequence analysis reveals regulation of genes in developing schistosomula of Schistosoma mansoni exposed to host portal serum

Contact Info

Product

Resources

About