The RNA-binding protein bicaudal C regulates polycystin 2 in the kidney by antagonizing <i>miR-17</i> activity

Tran, Uyen; Zakin, Lise; Schweickert, Axel; Agrawal, Raman; Döğer, Remziye; Blum, Martin; Robertis, E. M. De; Wessely, Oliver

doi:10.1242/jcs.070912

Cited by 40 publications

(77 citation statements)

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“…The first period of high TRPP2 expression at the late gastrula stage therefore coincides with the maximum increase of [Ca 2+ ] i in the kidney field of whole embryos (Leclerc et al, 2008). It is also interesting to note that the second period of high TRPP2 expression is correlated with tubule differentiation, where TRPP2 is known to play an important function (Tran et al, 2010).…”

Section: Resultsmentioning

confidence: 74%

“…TRPP2 in GRP plays a crucial role in the establishment of left-right asymmetry (Schweickert et al, 2007). At the tailbud stage, TRPP2 is also associated with cilia in another epithelium, the pronephric nephron tubule (Tran et al, 2010). We tested for the presence of cilia in cells from kidney field explants isolated at early (NF stage 13-14) or late (NF stage 18) neurula stage using anti-acetylated tubulin antibodies (supplementary material Fig.…”

Section: Resultsmentioning

confidence: 99%

“…To determine whether TRPP2 is required for kidney field specification, we analyzed whether pkd2 knockdown affects pax8 expression using previously validated pkd2-specific translationblocking antisense MOs (Pkd2-MO) (Tran et al, 2010). Pkd2-MO was injected at the four-cell stage into the two left blastomeres (2 pmoles per blastomere) in order to target the presumptive dorsolateral mesoderm containing kidney field precursors on the left side of the embryo.…”

Section: Resultsmentioning

confidence: 99%

“…Pkd2 mutants exhibit severe cardio-vascular defects, and form cysts in developing nephrons and pancreatic duct (Wu et al, 2000). Morpholino-based loss of function of pkd2 also results in the formation of pronephric cysts during zebrafish development (Obara et al, 2006;Sun et al, 2004) and in the formation of severe edema and of dilated pronephric tubules in Xenopus (Tran et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

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TRPP2-dependent Ca2+ signaling in dorso-lateral mesoderm is required for kidney field establishment inXenopus

Futel

Leclerc

Buisson

et al. 2015

Journal of Cell Science

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In Xenopus laevis embryos, kidney field specification is dependent on retinoic acid (RA) and coincides with a dramatic increase of Ca 2+ transients, but the role of Ca 2+ signaling in the kidney field is unknown. Here, we identify TRPP2, a member of the transient receptor potential (TRP) superfamily of channel proteins encoded by the pkd2 gene, as a central component of Ca 2+ signaling in the kidney field. TRPP2 is strongly expressed at the plasma membrane where it might regulate extracellular Ca 2+ entry. Knockdown of pkd2 in the kidney field results in the downregulation of pax8, but not of other kidney field genes (lhx1, osr1 and osr2). We further show that inhibition of Ca 2+ signaling with an inducible Ca 2+ chelator also causes downregulation of pax8, and that pkd2 knockdown results in a severe inhibition of Ca 2+ transients in kidney field explants. Finally, we show that disruption of RA results both in an inhibition of intracellular Ca 2+ signaling and of TRPP2 incorporation into the plasma membrane of kidney field cells. We propose that TRPP2-dependent Ca 2+ signaling is a key component of pax8 regulation in the kidney field downstream of RA-mediated nontranscriptional control of TRPP2.

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Section: Resultsmentioning

confidence: 74%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

TRPP2-dependent Ca2+ signaling in dorso-lateral mesoderm is required for kidney field establishment inXenopus

Futel

Leclerc

Buisson

et al. 2015

Journal of Cell Science

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“…TRPP2 is encoded by the PKD2 gene and in humans, mutations in PKD2 are responsible for autosomal dominant polycystic kidney disease (ADPKD) [67]. In addition, morpholino (MO)-based knock-down of pkd2 has been shown to result in the formation of pronephric cysts in zebrafish [68,69], and in the formation of severe oedema and dilated pronephric tubules in Xenopus [70].…”

Section: Trp Channels Are Involved In the Generation Of The Ca 2+ Sigmentioning

confidence: 99%

Ca2+ coding and decoding strategies for the specification of neural and renal precursor cells during development

et al. 2016

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Systematic analysis of migration factors by MigExpress identifies essential cell migration control genes in non‐small cell lung cancer

et al. 2021

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Cell migration is an essential process in health and in disease, including cancer metastasis. A comprehensive inventory of migration factors is nonetheless lacking—in part due to the difficulty in assessing migration using high‐throughput technologies. Hence, there are currently very few screens that systematically reveal factors controlling cell migration. Here, we introduce MigExpress as a platform for the ‘identification of Migration control genes by differential Expression’. MigExpress exploits the combination of in‐depth molecular profiling and the robust quantitative analysis of migration capacity in a broad panel of samples and identifies migration‐associated genes by their differential expression in slow‐ versus fast‐migrating cells. We applied MigExpress to investigate non‐small cell lung cancer (NSCLC), which is the most frequent cause of cancer mortality mainly due to metastasis. In 54 NSCLC cell lines, we comprehensively determined mRNA and protein expression. Correlating the transcriptome and proteome profiles with the quantified migration properties led to the discovery and validation of FLNC, DSE, CPA4, TUBB6, and BICC1 as migration control factors in NSCLC cells, which were also negatively correlated with patient survival. Notably, FLNC was the least expressed filamin in NSCLC, but the only one controlling cell migration and correlating with patient survival and metastatic disease stage. In our study, we present MigExpress as a new method for the systematic analysis of migration factors and provide a comprehensive resource of transcriptomic and proteomic data of NSCLC cell lines related to cell migration.

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The RNA-binding protein bicaudal C regulates polycystin 2 in the kidney by antagonizing miR-17 activity

Cited by 40 publications

References 1 publication

TRPP2-dependent Ca2+ signaling in dorso-lateral mesoderm is required for kidney field establishment inXenopus

TRPP2-dependent Ca2+ signaling in dorso-lateral mesoderm is required for kidney field establishment inXenopus

Ca2+ coding and decoding strategies for the specification of neural and renal precursor cells during development

Systematic analysis of migration factors by MigExpress identifies essential cell migration control genes in non‐small cell lung cancer

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