The rise of the nanomaterial metabolite corona, and emergence of the complete corona

Chetwynd, Andrew J.; Lynch, Iseult

doi:10.1039/c9en00938h

Cited by 62 publications

(59 citation statements)

References 149 publications

(208 reference statements)

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“…Whilst in theory, an analogous framework is expected to exist in the environmental corona, with greater heterogeneity in the structure and components, a wider range of molecules of different molecular weights and compositions are competing for the surface of the ENM. [24,43] To date, the structure-activity relationships in environmental corona formation on ENMs is not well understood, as it is dependent on the physicochemical properties of ENMs and chemical reactivities of eco-molecules.…”

Section: Environmental Corona Formation On Enms In Aquatic Environmenmentioning

confidence: 99%

“…Recently, it has also been suggested that metabolites may be an important co-constituent alongside proteins. [24] Secreted proteins are particularly important for microorganisms in water systems, which have a wide range of biological functions from self-protection to reproduction. [91,92] For example, cytochrome c (OmcA) is secreted by Shewanella oneidensis MR-1 (S. oneidensis MR-1), belonging to a bacterial extracellular protein.…”

Section: (6 Of 23)mentioning

confidence: 99%

“…[115] Antibiotics, bisphenol A, tetrachlorodibenzo-pdioxin, and other pollutants have been reported to be enriched in ENMs; thus, they are likely to induce synergistic or antagonistic effects in aquatic organisms. [24,[116][117][118] However, the bioavailability of ENMs may also be affected by the presence of these chemicals. [119]…”

Section: Other Substancesmentioning

confidence: 99%

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The Crucial Role of Environmental Coronas in Determining the Biological Effects of Engineered Nanomaterials

Wang

et al. 2020

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In aquatic environments, a large number of ecological macromolecules (e.g., natural organic matter (NOM), extracellular polymeric substances (EPS), and proteins) can adsorb onto the surface of engineered nanomaterials (ENMs) to form a unique environmental corona. The presence of environmental corona as an eco–nano interface can significantly alter the bioavailability, biocompatibility, and toxicity of pristine ENMs to aquatic organisms. However, as an emerging field, research on the impact of the environmental corona on the fate and behavior of ENMs in aquatic environments is still in its infancy. To promote a deeper understanding of its importance in driving or moderating ENM toxicity, this study systemically recapitulates the literature of representative types of macromolecules that are adsorbed onto ENMs; these constitute the environmental corona, including NOM, EPS, proteins, and surfactants. Next, the ecotoxicological effects of environmental corona‐coated ENMs on representative aquatic organisms at different trophic levels are discussed in comparison to pristine ENMs, based on the reported studies. According to this analysis, molecular mechanisms triggered by pristine and environmental corona‐coated ENMs are compared, including membrane adhesion, membrane damage, cellular internalization, oxidative stress, immunotoxicity, genotoxicity, and reproductive toxicity. Finally, current knowledge gaps and challenges in this field are discussed from the ecotoxicology perspective.

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Section: Environmental Corona Formation On Enms In Aquatic Environmenmentioning

confidence: 99%

Section: (6 Of 23)mentioning

confidence: 99%

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The Crucial Role of Environmental Coronas in Determining the Biological Effects of Engineered Nanomaterials

Wang

et al. 2020

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“…Furthermore, a limited number of studies have investigated a broader range of metabolites and demonstrated that a wide array of metabolites can interact with NMs to form a metabolite corona. [ 26–29 ] Yet, the concept of a small molecule corona, or indeed the realization that the presence of metabolites within the protein corona may mediate the NM behavior, has been largely overlooked in nanosafety to date. Here, characterization of the selective adsorption of small molecules to NMs, forming a metabolite corona, and the impact of proteins on the composition of the small molecule corona are presented.…”

Section: Introductionmentioning

confidence: 99%

The Nanomaterial Metabolite Corona Determined Using a Quantitative Metabolomics Approach: A Pilot Study

Chetwynd

Zhang

Thorn³

et al. 2020

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Nanomaterials (NMs) are promptly coated with biomolecules in biological systems leading to the formation of the so‐called corona. To date, research has predominantly focused on the protein corona and how it affects NM uptake, distribution, and bioactivity by conferring a biological identity to NMs enabling interactions with receptors to mediate cellular responses. Thus, protein corona studies are now integral to nanosafety assessment. However, a larger class of molecules, the metabolites, which are orders of magnitude smaller than proteins (<1000 Da) and regulate metabolic pathways, has been largely overlooked. This hampers the understanding of the bio–nano interface, development of computational predictions of corona formation, and investigations into uptake or toxicity at the cellular level, including identification of molecular initiating events triggering adverse outcome pathways. Here, a capillary electrophoresis–mass spectrometry based metabolomics approach reveals that pure polar ionogenic metabolite standards differentially adsorb to a range of 6 NMs (SiO2, 3 TiO2 with different surface chemistries, and naïve and carboxylated polystyrene NMs). The metabolite corona composition is quantitatively compared using protein‐free and complete plasma samples, revealing that proteins in samples significantly change the composition of the metabolite corona. This key finding provides the basis to include the metabolite corona in future nanosafety endeavors.

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“…The biological and environmental relevance of nanomaterial coronas is a central issue to advance nanobiosciences, nano(eco)toxicology, and nanosafety research in a broad sense [ 73 , 74 , 75 ]. In this regard, Nasser and Lynch (2016) [ 76 ] showed that protein eco-corona formation should be considered during toxicity assessment of nanomaterials with the D. magna model.…”

Section: Resultsmentioning

confidence: 99%

Effect of the Albumin Corona on the Toxicity of Combined Graphene Oxide and Cadmium to Daphnia magna and Integration of the Datasets into the NanoCommons Knowledge Base

et al. 2020

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In this work, we evaluated the effect of protein corona formation on graphene oxide (GO) mixture toxicity testing (i.e., co-exposure) using the Daphnia magna model and assessing acute toxicity determined as immobilisation. Cadmium (Cd2+) and bovine serum albumin (BSA) were selected as co-pollutant and protein model system, respectively. Albumin corona formation on GO dramatically increased its colloidal stability (ca. 60%) and Cd2+ adsorption capacity (ca. 4.5 times) in reconstituted water (Daphnia medium). The acute toxicity values (48 h-EC50) observed were 0.18 mg L−1 for Cd2+-only and 0.29 and 0.61 mg L−1 following co-exposure of Cd2+ with GO and BSA@GO materials, respectively, at a fixed non-toxic concentration of 1.0 mg L−1. After coronation of GO with BSA, a reduction in cadmium toxicity of 110 % and 238% was achieved when compared to bare GO and Cd2+-only, respectively. Integration of datasets associated with graphene-based materials, heavy metals and mixture toxicity is essential to enable re-use of the data and facilitate nanoinformatics approaches for design of safer nanomaterials for water quality monitoring and remediation technologies. Hence, all data from this work were annotated and integrated into the NanoCommons Knowledge Base, connecting the experimental data to nanoinformatics platforms under the FAIR data principles and making them interoperable with similar datasets.

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The rise of the nanomaterial metabolite corona, and emergence of the complete corona

Abstract: Metabolites present in the corona may play a key role in biological recognition of nanomaterials yet remain a much-overlooked aspect of the corona.

Cited by 62 publications

References 149 publications

The Crucial Role of Environmental Coronas in Determining the Biological Effects of Engineered Nanomaterials

The Crucial Role of Environmental Coronas in Determining the Biological Effects of Engineered Nanomaterials

The Nanomaterial Metabolite Corona Determined Using a Quantitative Metabolomics Approach: A Pilot Study

Effect of the Albumin Corona on the Toxicity of Combined Graphene Oxide and Cadmium to Daphnia magna and Integration of the Datasets into the NanoCommons Knowledge Base

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