The rise of necrotrophic effectors

Kanyuka, K.; Igna, Alina-Andrada; Solomon, Peter S.; Oliver, Richard P.

doi:10.1111/nph.17811

Cited by 11 publications

(7 citation statements)

References 18 publications

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“…upregulated at two dpi. These genes were not considered candidates because previous QTL mapping has shown that R-genes in this region are not associated with R. solani resistance ) and that R-genes are associated with necrotrophic susceptibility rather than resistance in several cases (Hammond-Kosack & Rudd 2008;Shao et al 2021;Kanyuka et al 2022). Several biotic stress scenarios, including resistance against aggressive B. cinerea (Gai et al 2018), Colletotrichum acutatum and C. gloeosporioides (Adhikari et al 2021), have implicated MLPs.…”

Section: Resultsmentioning

confidence: 99%

“…Necrotrophic fungi that may benefit from cell death have evolved ways to regulate immune responses to their advantage. Most known examples have been found from stem-or leaf-infecting pathogens (Hammond-Kosack & Rudd 2008;Shao et al 2021;Kanyuka et al 2022). Pyrenophora tritici-repentis, a necrotrophic fungus of wheat, encodes a TOX-A protein that localizes to chloroplasts in wheat and induces light-dependent ROS bursts that lead to cell death (Manning et al 2009;Andersen et al 2021).…”

Section: Chloroplast and Mitochondrial Targetingmentioning

confidence: 99%

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Host organelles and transporters in underground plant-pathogen interactions

Dölfors

2024

Acta Universitatis Agriculturae Sueciae

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This thesis covers studies of three different soilborne plant pathogens, the two fungi, Rhizoctonia solani and Verticillium longisporum, and the protist Plasmodiophora brassicae, as well as their host responses. Based on genome sequence analysis of the pathogens and their plant hosts, different effectors and plant defence factors were predicted. Follow-up molecular studies revealed the following: In sugar beets, two genes encoding major latex protein-like (MLP) family members, MLP1 and MLP3, contribute to the defence against R. solani. The small cysteine-rich effector RsRCP1 was highly induced in the fungus upon infection. RsRCP1 was localized to chloroplasts and mitochondria in the leaves of Nicotiana benthamiana. An additional MLP gene in oilseed rape, MLP6, was found to provide elevated levels of defence to V. longisporum together with a nitrate/peptide transporter family protein (NPF5.12). Recognition of the fungus triggered nitrate starvation and MLP-mediated defence, together reducing the lipophilic suberin barrier in the endodermal cell walls. In the genome of the clubroot pathogen P. brassicae, a consensus sequence led to the identification of peroxisomal targeting effectors. Arabidopsis mutants with impaired peroxisomal biogenesis demonstrated the importance of the plant peroxisomal transport proteins for P. brassicae establishment in the root. Host peroxisomal proteins embodied in the resting spores were also identified using a transgenic peroxisomal marker line of Arabidopsis. New technological advances and possibilities for genetic engineering of these three pathogens would greatly contribute to a deeper understanding of these different pathological systems.

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Section: Resultsmentioning

confidence: 99%

Section: Chloroplast and Mitochondrial Targetingmentioning

confidence: 99%

Host organelles and transporters in underground plant-pathogen interactions

Dölfors

2024

Acta Universitatis Agriculturae Sueciae

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“…For a gene-for-gene pathogen–host interaction type, the ‘gene-for-gene phenotype’ metagenotype workflow is followed (a gene-for-gene interaction is when a known genetic interaction is conferred by a specific pathogen avirulence gene product and its cognate host resistance gene product) ( Figure 2c and d , further described in the figure legend Flor, 1956 ; Jones and Dangl, 2006 ; Kanyuka et al, 2022 ). The metagenotypes and phenotype annotations are made in the same way as the standard ‘pathogen–host interaction phenotype’ workflow, but with different supporting data.…”

Section: Resultsmentioning

confidence: 99%

A framework for community curation of interspecies interactions literature

Cuzick

Seager

Wood

et al. 2023

eLife

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The quantity and complexity of data being generated and published in biology has increased substantially, but few methods exist for capturing knowledge about phenotypes derived from molecular interactions between diverse groups of species, in such a way that is amenable to data-driven biology and research. To improve access to this knowledge, we have constructed a framework for the curation of the scientific literature studying interspecies interactions, using data curated for the Pathogen–Host Interactions database (PHI-base) as a case study. The framework provides a curation tool, phenotype ontology, and controlled vocabularies to curate pathogen–host interaction data, at the level of the host, pathogen, strain, gene, and genotype. The concept of a multispecies genotype, the ‘metagenotype,’ is introduced to facilitate capturing changes in the disease-causing abilities of pathogens, and host resistance or susceptibility, observed by gene alterations. We report on this framework and describe PHI-Canto, a community curation tool for use by publication authors.

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“…Curating experiments with a gene-for-gene relationship For a gene-for-gene pathogen-host interaction type (when a known genetic interaction is conferred by a specific pathogen avirulence gene product and its cognate host resistance gene product) (Figure 2c, d, further described in the figure legend) (Flor, 1956;Jones & Dangl, 2006;Kanyuka, Igna, Solomon, & Oliver, 2022) the 'gene-for-gene phenotype' metagenotype workflow is followed. The metagenotypes and phenotype annotations are made in the same way as the standard 'pathogen-host interaction phenotype' workflow, but with different supporting data.…”

Section: Curating Pathogen Effector Experimentsmentioning

confidence: 99%

A framework for community curation of interspecies interactions literature

Cuzick

Seager

Wood

et al. 2022

Preprint

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The quantity and complexity of data being generated and published in biology has increased substantially, but few methods exist for capturing knowledge about phenotypes derived from molecular interactions between diverse groups of species, in such a way that is amenable to data-driven biology and research. To improve access to this knowledge, we have constructed a framework for the curation of the scientific literature studying interspecies interactions, using data curated for the Pathogen-Host Interactions Database (PHI-base) as a case study. The framework provides a curation tool, phenotype ontology and controlled vocabularies to curate pathogen-host interaction data (at the level of the host, pathogen, strain, gene and genotype). The concept of a multispecies genotype, the 'metagenotype', is introduced to facilitate capturing changes in the pathogens' disease-causing abilities, and host resistance or susceptibility observed by gene alterations. We report on this framework and describe PHI-Canto, a community curation tool for use by publication authors.

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The rise of necrotrophic effectors

Cited by 11 publications

References 18 publications

Host organelles and transporters in underground plant-pathogen interactions

Host organelles and transporters in underground plant-pathogen interactions

A framework for community curation of interspecies interactions literature

A framework for community curation of interspecies interactions literature

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