The Rigid Layer of the Cell Wall of Escherichia coli Strain B

Weidel, Wolfhard; Hensel, Frank; Martin, Hans Herbert

doi:10.1099/00221287-22-1-158

Cited by 282 publications

(135 citation statements)

References 9 publications

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“…Absorbance was detected at 205 nm and separation of muropeptides was achieved using 50 mM sodium phosphate, pH 4.35, ϩ 0.4% (v/v) sodium azide for solvent A, and 75 mM sodium phosphate, pH 4.95, ϩ 15% (v/v) methanol for solvent B. Peaks were identified based on retention time, which has been extensively established using amino acid analysis, enzymatic digestion, Edman degradation, radioactive labeling, dansylation, paper chromatography, reduction, and altering temperature, pH, and ionic strength (25,26,(32)(33)(34)(35)(36)(37)(38)(39)(40). Flow was set to 125 l/min with a linear gradient over 50 min to complete elution of all muropeptides within 25 min.…”

Section: Methodsmentioning

confidence: 99%

High-throughput, Highly Sensitive Analyses of Bacterial Morphogenesis Using Ultra Performance Liquid Chromatography

Desmarais

Tropini

Miguel

et al. 2015

Journal of Biological Chemistry

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Background: HPLC enables quantification of bacterial cell-wall composition, yet systematic studies across strains, species, and chemical perturbations are lacking. Results: UPLC coupled to computational modeling enables submicroliter injection volumes, and was applied to systematic analysis of several Gram-negative species. Conclusion: Composition is largely decoupled from morphology, although large interspecies differences were evident. Significance: UPLC and automated analysis accelerate discovery regarding peptidoglycan and physiology.

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Section: Methodsmentioning

confidence: 99%

High-throughput, Highly Sensitive Analyses of Bacterial Morphogenesis Using Ultra Performance Liquid Chromatography

Desmarais

Tropini

Miguel

et al. 2015

Journal of Biological Chemistry

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“…Salton, 1958;Noller & Hartsell, 1961 a, b) considered that EDTA disorganized one of the layers, probably lipoprotein, which overlie and protect the mucopeptide substrate of lysozyme in the cell walls of Gram-negative bacteria (Weidel, Frank & Martin, 1960). Thus, the purified mucopeptides of these bacteria were completely dissolved by lysozyme without the addition of EDTA (Weidel et al 1960; Mandelstam, 1962). In the cases of various pathogenic Salmonella organisms, the action of EDTA in making the mucopeptide accessible to lysozyme was found (Colobert, 1957a, b) to involve the release of lipid material, believed to come from the 0 antigens of the cell walls.…”

Section: Introductionmentioning

confidence: 99%

The Effect of Ethylenediaminetetra-acetic Acid on the Cell Walls of Some Gram-Negative Bacteria

Gray¹,

Wilkinson²

1965

Journal of General Microbiology

133

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SUMMARYA comparative survey of amino acid, amino sugar, sugar and lipid components of the cell walls of strains of Fseudomonas aeruginosa, Alcaligelzes faecalis, Escherichia coli and Proteus mirabilis was made. The cell walls of P. aeruginosa and A. faecalis, against which ethylenediaminetetra-acetic acid (EDTA) has a potent bactericidal action, differed from those of the other organisms principally in their sugar components and in their high content of phosphorus. EDTA at alkaline pH selectively solubilized a high proportion of the carbohydrate and phosphorus present, apparently as lipopolysaccharides, in the walls of sensitive organisms. It is suggested that metal cations and lipopolysaccharides in the cell walls of P. aeruginosa and A. faecalis may be essential to the structural integrity of these organisms.

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“…The experiments of Young, Spizizen & Crawford (1963) and Young (1965) suggest that competent Bacillus subtilis cells (those capable of reacting with DNA) do have slight modifications in their cell-wall carbohydrate composition when compared to walls derived from similar but non-competent cells. The Gram-negative bacterial cell wall is a complex structure and its carbohydrate composition can be modified qualitatively by modifying the carbohydrates present in the growth medium (Weidel, Frank & Martin, 1960;Nikaido, 1962). Lactate has been shown to be a precursor of carbohydrates (Krebs, Dierks & Gascoyne, 1964;Lardy, Paetkau & Walter, 1965) and therefore, has the potential of causing such modifications in the cell-wall composition of Haemophilus injluenzae.…”

Section: Results a N D Discussionmentioning

confidence: 99%

Specificity of Lactate for the Development of Competence in Haemophilus influenzae

Ranhand¹

1970

Journal of General Microbiology

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SUMMARYA number of common carbohydrates and polyols were tested to see if they could replace the lactate requirement for the development of competence in cultures of Haemophilus injluenzae. Pyruvate, fructose, and glucose, when tested in the presence of inosine, were slightly stimulatory. They gave respectively, 29, 25 and 13 % of the control value obtained with inosine and lactate. Under similar test conditions, mannose, mannitol, galactose, sorbose, sorbitol, ribose, glucosamine, rhamnose, D (-) arabinose, L (+) arabinose, xylose and fucose were inactive.In combination experiments, glucose, inosine and pyruvate, and fructose, inosine and pyruvate stimulated the development of competence to 50 to 70 % of the inosine and lactate control value. Without inosine, the stimulation due to glucose and pyruvate, and fructose and pyruvate was 3 and 9 %, respectively.

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The Rigid Layer of the Cell Wall of Escherichia coli Strain B

Cited by 282 publications

References 9 publications

High-throughput, Highly Sensitive Analyses of Bacterial Morphogenesis Using Ultra Performance Liquid Chromatography

High-throughput, Highly Sensitive Analyses of Bacterial Morphogenesis Using Ultra Performance Liquid Chromatography

The Effect of Ethylenediaminetetra-acetic Acid on the Cell Walls of Some Gram-Negative Bacteria

Specificity of Lactate for the Development of Competence in Haemophilus influenzae

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