The ribulose monophosphate pathway operon encoding formaldehyde fixation in a thermotolerant methylotroph,<i>Bacillus brevis</i>S1

Yurimoto, Hiroya; Hirai, Reiko; Yasueda, Hisashi; Mitsui, Ryoji; Sakai, Yasuyoshi; Kato, Nobuo

doi:10.1111/j.1574-6968.2002.tb11345.x

Cited by 26 publications

(4 citation statements)

References 16 publications

(29 reference statements)

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“…Recently, genetic studies on the ribulose monophosphate (RuMP) pathway for formaldehyde fixation have been performed with methylotrophic bacteria, Methylomonas aminofaciens 77a (15), Mycobacterium gastri MB19 (9), and Bacillus brevis S1 (25). A homology search of protein databases revealed that the 3-hexulose-6-phosphate synthase (HPS) and 6-phospho-3-hexuloisomerase (PHI) enzymes in the RuMP pathway exhibit high levels of similarity to a variety of unidentified proteins of nonmethylotrophic prokaryotes, including members of the domains Bacteria and Archaea (22), as well as methylotrophs.…”

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confidence: 99%

Formaldehyde Fixation Contributes to Detoxification for Growth of a Nonmethylotroph, Burkholderia cepacia TM1, on Vanillic Acid

Mitsui

Kusano

Yurimoto

et al. 2003

Appl Environ Microbiol

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During bacterial degradation of methoxylated lignin monomers, such as vanillin and vanillic acid, formaldehyde is released through the reaction catalyzed by vanillic acid demethylase. When Burkholderia cepacia TM1 was grown on vanillin or vanillic acid as the sole carbon source, the enzymes 3-hexulose-6-phosphate synthase (HPS) and 6-phospho-3-hexuloisomerase (PHI) were induced. These enzymes were also expressed during growth on Luria-Bertani medium containing formaldehyde. To understand the roles of these enzymes, the hps and phi genes from a methylotrophic bacterium, Methylomonas aminofaciens 77a, were introduced into B. cepacia TM1. The transformant strain constitutively expressed the genes for HPS and PHI, and these activities were two-or threefold higher than the activities in the wild strain. Incorporation of [ 14 C]formaldehyde into the cell constituents was increased by overexpression of the genes. Furthermore, the degradation of vanillic acid and the growth yield were significantly improved at a high concentration of vanillic acid (60 mM) in the transformant strain. These results suggest that HPS and PHI play significant roles in the detoxification and assimilation of formaldehyde. This is the first report that enhancement of the HPS/PHI pathway could improve the degradation of vanillic acid in nonmethylotrophic bacteria.Lignin is the most abundant aromatic compound in the biosphere. Therefore, there have been various studies of its use. Chemical degradation of lignin produces a high yield of vanillin (8), which is an important starting material for the production of various flavors and fragrances (13). The bacterial pathway for vanillin degradation has been extensively investigated (12). In the early steps of the pathway, vanillin is oxidized to vanillic acid, whose aromatic methyl ether is demethylated through hydroxylation to yield protocatechuic acid and formaldehyde. In contrast to the degradation pathway for protocatechuic acid (11), the fate of formaldehyde in vanillin-utilizing microorganisms has not been studied. Nevertheless, lignin is recognized to be a source of formaldehyde present in nature (3,20).In methylotrophic microorganisms, formaldehyde is a key intermediate in the metabolism of several C 1 compounds and enters into both dissimilation and assimilation pathways. On the other hand, formaldehyde is a highly reactive compound that has a toxic effect on all organisms through its nonspecific reactivity with proteins and nucleic acids (5, 6). In methylotrophic organisms, both dissimilatory and assimilatory pathways for formaldehyde could contribute to the detoxification of formaldehyde (16).Recently, genetic studies on the ribulose monophosphate (RuMP) pathway for formaldehyde fixation have been performed with methylotrophic bacteria, Methylomonas aminofaciens 77a (15), Mycobacterium gastri MB19 (9), and Bacillus brevis S1 (25). A homology search of protein databases revealed that the 3-hexulose-6-phosphate synthase (HPS) and 6-phospho-3-hexuloisomerase (PHI) enzymes in the RuMP pathway ex...

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Formaldehyde Fixation Contributes to Detoxification for Growth of a Nonmethylotroph, Burkholderia cepacia TM1, on Vanillic Acid

Mitsui

Kusano

Yurimoto

et al. 2003

Appl Environ Microbiol

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“…В кишечном микробиоме кроликов из II опытной группы по сравнению с I группой происходила активация (до 4-кратной, p  0,05) 8 путей, которые относились к белковому обмену (биосинтез и превращение аминокислот, азотистых соединений), 4 путей, относящихся к углеводному обмену (расщепление различных сахаров), 3 -к энергетическому обмену (метилцитратный цикл и гликолиз), 2 -к биосинтезу спиртов, 1 -к фотодыханию , 1 -к ассимиляции формальдегида, 1 -к деградации мио-, хиро-и сцилло-инозитола, 1 -к синтезу клеточной стенки и спорообразованию (путь биосинтеза тейховых кислот). Интересно, что доминирующее число потенциальных метаболических путей (15 путей) было связано с деградацией ароматических соединений и ксенобиотиков, включая деградацию таких токсикантов, как катехин, формальдегид, 3-фенилпропаноат, олигомер нейлона-6 (73,74). Усиление потенциала деградации ксенобиотиков в кишечнике кроликов из опытной группы могло иметь связь с увеличением численности бактерий Bacillus spp.…”

Section: среднесуточный прирост живой массы у кроликов (Oryctolagus D...unclassified

СОСТАВ И ФУНКЦИИ МИКРОБИОМА СЛЕПЫХ ОТРОСТ-КОВ КИШЕЧНИКА Oryctolagus dominis ПОД ВЛИЯНИЕМ КОМ-ПЛЕКСНОЙ КОРМОВОЙ ДОБАВКИ

НАРКЕВИЧ¹

2023

S-h. biol.

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“…However, assimilation of these alternative resources also poses problem with carbon conversation. As an example, the ribulose-5-monophosphate pathway (RuMP) that is present in methylotrophic bacteria and in some yeasts such as Pichia pastoris (Yurimoto et al, 2002;Muller et al, 2015;Russmayer et al, 2015) is one of the three natural methanol assimilatory pathways known so far (Zhang et al, 2017) which can produce one acetyl-CoA from three formaldehydes at the expense of the decarboxylation of pyruvate. Liao's team (Bogorad et al, 2014) showed that the decarboxylation step could be avoided by combining the RuMP with phosphoketolase enzymes, leading to the production of acetyl-CoA from 2 formaldehydes through a methanol condensation cycle (MCC) as depicted in Figure 2.…”

Section: The Methanol Condensation Cycle For Methanol Assimilation Wimentioning

confidence: 99%

Synthetic Biology Applied to Carbon Conservative and Carbon Dioxide Recycling Pathways

François

Lachaux²,

Morin

2020

Front. Bioeng. Biotechnol.

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The global warming conjugated with our reliance to petrol derived processes and products have raised strong concern about the future of our planet, asking urgently to find sustainable substitute solutions to decrease this reliance and annihilate this climate change mainly due to excess of CO 2 emission. In this regard, the exploitation of microorganisms as microbial cell factories able to convert non-edible but renewable carbon sources into biofuels and commodity chemicals appears as an attractive solution. However, there is still a long way to go to make this solution economically viable and to introduce the use of microorganisms as one of the motor of the forthcoming bio-based economy. In this review, we address a scientific issue that must be challenged in order to improve the value of microbial organisms as cell factories. This issue is related to the capability of microbial systems to optimize carbon conservation during their metabolic processes. This initiative, which can be addressed nowadays using the advances in Synthetic Biology, should lead to an increase in products yield per carbon assimilated which is a key performance indice in biotechnological processes, as well as to indirectly contribute to a reduction of CO 2 emission.

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The ribulose monophosphate pathway operon encoding formaldehyde fixation in a thermotolerant methylotroph,Bacillus brevisS1

Cited by 26 publications

References 16 publications

Formaldehyde Fixation Contributes to Detoxification for Growth of a Nonmethylotroph, Burkholderia cepacia TM1, on Vanillic Acid

Formaldehyde Fixation Contributes to Detoxification for Growth of a Nonmethylotroph, Burkholderia cepacia TM1, on Vanillic Acid

СОСТАВ И ФУНКЦИИ МИКРОБИОМА СЛЕПЫХ ОТРОСТ-КОВ КИШЕЧНИКА Oryctolagus dominis ПОД ВЛИЯНИЕМ КОМ-ПЛЕКСНОЙ КОРМОВОЙ ДОБАВКИ

Synthetic Biology Applied to Carbon Conservative and Carbon Dioxide Recycling Pathways

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