To the best of our knowledge, this is the first report describing the combined cremaster muscle and pubic bone flap model in the rat. It seems to have an important advantage over the other composite osteomuscular flap models in terms of the cremaster muscle being suitable for the intravital microscopy. Besides being harvested from a small animal species, it is reliable, versatile, and relatively easy to dissect with quite long isolated vascular pedicle, which allows a greater range of flap displacement. It can be used for different applicability including microcirculatory, pharmacologic, physiological, biochemical, and immunologic studies, as well as for the transplantation studies.