The rhizosphere and root exudates of maize seedlings drive plasmid mobilization in soil

Zhu, Hangfei; Zhang, Li; Li, Shanshan; Wang, Yujing; Sun, Shili; Chen, Jun; Kou, Shumeng; Jin, Qinian; Xiao, Ming

doi:10.1016/j.apsoil.2017.10.039

Cited by 12 publications

(3 citation statements)

References 26 publications

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“…Although fructose also occurs in maize roots (Bernklau & Bjostad, 2008) larval responses to fructose were generally (qualitatively) weaker than the responses to the blend with GFS, although in the choice test larvae chose equally between a blend with GFS and the blend with only fructose. Glucose is the most abundant sugar in maize roots (Bernklau & Bjostad, 2008;Kraffczyk, Trolldenier, & Beringer, 1984;Zhu et al, 2018), but in our experiments, the blends with glucose did not elicit strong feeding by the larvae.…”

Section: Discussioncontrasting

confidence: 64%

Sugar preferences of western corn rootworm larvae in a feeding stimulant blend

Bernklau

Hibbard

Bjostad

2018

J Applied Entomology

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Feeding behaviour, feeding intensity and staying behaviour of neonate western corn rootworm larvae (Diabrotica virgifera virgifera LeConte) were evaluated in response to synthetic feeding stimulant blends to determine larval preferences among the three maize root sugars (glucose, fructose and sucrose) in the active blend and to determine whether any single sugar can substitute for the 3‐sugar combination in a feeding stimulant blend. These experiments demonstrated the strong affinity that western corn rootworm larvae have for the natural sugars found in maize roots and also showed that sucrose is the most preferred of the three primary maize root sugars. The blend containing sucrose at 30 mg/ml elicited feeding that was not significantly different than the natural glucose:fructose:sucrose blend. In subtraction bioassays, removal of sucrose from the blend resulted in significantly fewer larvae feeding. When the three‐sugar blend was substituted with one of the number of various mono‐, di‐ or trisaccharides, fewer larvae fed on all of the treatments compared to the blend with sucrose, except for the blend with maltose. In feeding choice tests, larvae preferred a blend containing sucrose over blends with either glucose or fructose, but larvae chose equally between a blend with sucrose and a blend containing the three‐sugar mixture found in maize roots. Based on these results, a feeding stimulant blend with glucose (30 mg/ml), fructose (4 mg/ml) and sucrose (4 mg/ml) elicits the strongest feeding response, but sucrose alone, in amounts equivalent to the total maize root sugar concentration (30 mg/ml), could serve as a substitute for the 3‐sugar mixture in a synthetic feeding stimulant blend.

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Section: Discussioncontrasting

confidence: 64%

Sugar preferences of western corn rootworm larvae in a feeding stimulant blend

Bernklau

Hibbard

Bjostad

2018

J Applied Entomology

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“…The capacity of an environmental and naturally transformable E. coli strain like ED1 to survive in soil and colonize the plant rhizosphere has relevant implications in the light of the antibiotic cycle and the One Health vision. The plant rhizosphere is indeed a well characterized, substrate-rich, hot spot for bacterial activity and abundance (Zhu et al, 2018), where naturally competent cells can find higher concentrations of free DNA and could, moreover, reach the growth phase in which transformation occurs with high frequency (Sørensen and Jensen, 1998;Mølbak et al, 2003;Ling et al, 2016;Zhu et al, 2018). Relevant concentrations of ARGs can reach the plant rhizosphere, e.g., through soil amended with manure, sewage sludge and treated wastewater (Chen et al, 2017;Riva et al, 2020;Wu et al, 2020).…”

Section: Discussionmentioning

confidence: 99%

An Environmental Escherichia coli Strain Is Naturally Competent to Acquire Exogenous DNA

Riva

Eckert

et al. 2020

Front. Microbiol.

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The diffusion of antibiotic resistance determinants in different environments, e.g., soil and water, has become a public concern for global health and food safety and many efforts are currently devoted to clarify this complex ecological and evolutionary issue. Horizontal gene transfer (HGT) has an important role in the spread of antibiotic resistance genes (ARGs). However, among the different HGT mechanisms, the capacity of environmental bacteria to acquire naked exogenous DNA by natural competence is still poorly investigated. This study aimed to characterize the ability of the environmental Escherichia coli strain ED1, isolated from the crustacean Daphnia sp., to acquire exogenous DNA by natural competence. Transformation experiments were carried out varying different parameters, i.e., cell growth phase, amount of exogenous DNA and exposition to artificial lake water (ALW) and treated wastewater to mimic environmentallike conditions that may be encountered in the agri-food system. Results were compared with those showed by the laboratory E. coli strain DH5α. Our experimental data, supported by genomic sequencing, showed that, when exposed to pure water, ED1 strain was able to acquire exogenous DNA with frequencies (10 −8-10 −9) statistically higher than the ones observed for DH5α strain (10 −10). Interestingly, higher values were retrieved for ED1 than DH5α strains exposed to ALW (10 −7 vs. 10 −9 , respectively) or treated wastewater (10 −8 vs. 10 −10 , respectively). We tested, therefore, ED1 strain ability to colonize the rhizosphere of lettuce, a model plant representative of rawconsumed vegetables of high economic importance in the ready-to-eat food industry. Results showed that ED1 strain was able to efficiently colonize lettuce rhizosphere, revealing a stable colonization for 14 days-long period. In conclusion, ED1 strain ability to acquire exogenous DNA in environmental-like conditions by natural competence, combined with its ability to efficiently and stably colonize plant rhizosphere, poses the attention to food and human safety showing a possible route of diffusion of antibiotic resistance in the agri-food system, sustaining the "One Health" warnings related to the antibiotic spread.

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“…Furthermore, root exudates can promote microbial degradation near the rhizosphere [ 40 ]. In this study, 18 compounds, including the most widely used nitrogen fertilizer [ 52 ] and 17 kinds of rhizosphere secretory substances of maize, were selected ( Table S12 ) [ 53 , 54 ] as external addictive conditions to investigate their influence on the degradability of S-TH substitutes.…”

Section: Methodsmentioning

confidence: 99%

Triazine Herbicides Risk Management Strategies on Environmental and Human Health Aspects Using In-Silico Methods

Yao

Sun

Zhao

2023

IJMS

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As an effective herbicide, 1, 3, 5-Triazine herbicides (S-THs) are used widely in the pesticide market. However, due to their chemical properties, S-THs severely threaten the environment and human health (e.g., human lung cytotoxicity). In this study, molecular docking, Analytic Hierarchy Process—Technique for Order Preference by Similarity to the Ideal Solution (AHP-TOPSIS), and a three-dimensional quantitative structure-active relationship (3D-QSAR) model were used to design S-TH substitutes with high herbicidal functionality, high microbial degradability, and low human lung cytotoxicity. We discovered a substitute, Derivative-5, with excellent overall performance. Furthermore, Taguchi orthogonal experiments, full factorial design of experiments, and the molecular dynamics method were used to identify three chemicals (namely, the coexistence of aspartic acid, alanine, and glycine) that could promote the degradation of S-THs in maize cropping fields. Finally, density functional theory (DFT), Estimation Programs Interface (EPI), pharmacokinetic, and toxicokinetic methods were used to further verify the high microbial degradability, favorable aquatic environment, and human health friendliness of Derivative 5. This study provided a new direction for further optimizations of novel pesticide chemicals.

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The rhizosphere and root exudates of maize seedlings drive plasmid mobilization in soil

Cited by 12 publications

References 26 publications

Sugar preferences of western corn rootworm larvae in a feeding stimulant blend

Sugar preferences of western corn rootworm larvae in a feeding stimulant blend

An Environmental Escherichia coli Strain Is Naturally Competent to Acquire Exogenous DNA

Triazine Herbicides Risk Management Strategies on Environmental and Human Health Aspects Using In-Silico Methods

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