The RGD motif is involved in CD97/ADGRE5-promoted cell adhesion and viability of HT1080 cells

Tjong, Wen-Ye; Lin, Hsi‐Hsien

doi:10.1038/s41598-018-38045-w

Cited by 17 publications

(13 citation statements)

References 43 publications

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“…MEOX1 plays key role in regulating somite development, which associated with cancer progression [54]. ADGRE5 was closely related with tumor cell adhesion, migration, angiogenesis, and apoptosis [55]. These key genes play important roles in glioma proliferation.…”

Section: Resultsmentioning

confidence: 99%

Stoichioproteomics reveal oxygen usage bias, key proteins and pathways in glioma

Yin

Mou

et al. 2019

BMC Med Genomics

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Background The five-year survival rate and therapeutic effect of malignant glioma is low. Identification of key/associated proteins and pathways in glioma is necessary for developing effective diagnosis and targeted therapy of glioma. In addition, Glioma involves hypoxia-specific microenvironment, whether hypoxia restriction influences the stoichioproteomic characteristics of expressed proteins is unknown. Methods In this study, we analyzed the most comprehensive immunohistochemical data from 12 human glioma samples and 4 normal cell types of cerebral cortex, identified differentially expressed proteins (DEPs), and researched the oxygen contents of DEPs, highly and lowly expressed proteins. Further we located key genes on human genome to determine their locations and enriched them for key functional pathways. Results Our results showed that although no difference was detected on whole proteome, the average oxygen content of highly expressed proteins is 6.65% higher than that of lowly expressed proteins in glioma. A total of 1480 differentially expressed proteins were identified in glioma, including 226 up regulated proteins and 1254 down regulated proteins. The average oxygen content of up regulated proteins is 2.56% higher than that of down regulated proteins in glioma. The localization of differentially expressed genes on human genome showed that most genes were on chromosome 1 and least on Y. The up regulated proteins were significantly enriched in pathways including cell cycle, pathways in cancer, oocyte meiosis, DNA replication etc. Functional dissection of the up regulated proteins with high oxygen contents showed that 51.28% of the proteins were involved in cell cycle and cyclins. Conclusions Element signature of oxygen limitation could not be detected in glioma, just as what happened in plants and microbes. Unsaved use of oxygen by the highly expressed proteins and DEPs were adapted to the fast division of glioma cells. This study can help to reveal the molecular mechanism of glioma, and provide a new approach for studies of cancer-related biomacromolecules. In addition, this study lays a foundation for application of stoichioproteomics in precision medicine. Electronic supplementary material The online version of this article (10.1186/s12920-019-0571-y) contains supplementary material, which is available to authorized users.

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Section: Resultsmentioning

confidence: 99%

Stoichioproteomics reveal oxygen usage bias, key proteins and pathways in glioma

Yin

Mou

et al. 2019

BMC Med Genomics

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“…The second is glycosaminoglycan chondroitin sulfate, which binds specifically to the large isoform(s) of CD97 and affects cell attachment [82,83]. The third category of ligands includes integrins, such as α5β1 and αvβ3, which bind a (arginine-glycine-aspartic acid) RGD motif in the stalk region of the CD97 α-chain 19 [84][85][86].…”

Section: Of 32mentioning

confidence: 99%

CD36 and CD97 in Pancreatic Cancer versus Other Malignancies

Tănase

Gheorghișan-Gălățeanu

Popescu

et al. 2020

IJMS

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Starting from the recent identification of CD36 and CD97 as a novel marker combination of fibroblast quiescence in lung during fibrosis, we aimed to survey the literature in search for facts about the separate (or concomitant) expression of clusters of differentiation CD36 and CD97 in either tumor- or pancreatic-cancer-associated cells. Here, we provide an account of the current knowledge on the diversity of the cellular functions of CD36 and CD97 and explore their potential (common) contributions to key cellular events in oncogenesis or metastasis development. Emphasis is placed on quiescence as an underexplored mechanism and/or potential target in therapy. Furthermore, we discuss intricate signaling mechanisms and networks involving CD36 and CD97 that may regulate different subpopulations of tumor-associated cells, such as cancer-associated fibroblasts, adipocyte-associated fibroblasts, tumor-associated macrophages, or neutrophils, during aggressive pancreatic cancer. The coexistence of quiescence and activated states in cancer-associated cell subtypes during pancreatic cancer should be better documented, in different histological forms. Remodeling of the local microenvironment may also change the balance between growth and dormant state. Taking advantage of the reported data in different other tissue types, we explore the possibility to induce quiescence (similar to that observed in normal cells), as a therapeutic option to delay the currently observed clinical outcome.

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“…Importantly, both cells used in Wandel’s study, activated endothelial and CHO cells, express integrins (Kim et al, 2002; Ward et al, 2018). Nowadays, many studies keep reporting CD97/integrin interaction (Ward et al, 2018; Tjong and Lin, 2019), whereas no recent evidence about binding of CD97 to Thy-1/CD90 has been reported after Wandel’s description in 2012, supporting the hypothesis that Thy-1/CD90 might be a regulatory element of the CD97/integrin interaction through the regulation of integrin activity. Such mode of regulation was recently reported by Fiore et al (2015) in a different cellular model.…”

Section: Thy-1/cd90 In Cell-to-cell Interactionsmentioning

confidence: 99%

Thy-1/CD90 a Bidirectional and Lateral Signaling Scaffold

Leyton

Díaz

Martı́nez

et al. 2019

Front. Cell Dev. Biol.

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Thy-1/CD90 is a glycoprotein attached to the outer face of the plasma membrane with various functions, which depend on the context of specific physiological or pathological conditions. Many of these reported functions for Thy-1/CD90 arose from studies by our group, which identified the first ligand/receptor for Thy-1/CD90 as an integrin. This finding initiated studies directed toward unveiling the molecular mechanisms that operate downstream of Thy-1/CD90 activation, and its possible interaction with proteins in the membrane plane to regulate their function. The association of Thy-1/CD90 with a number of cell surface molecules allows the formation of extra/intracellular multiprotein complexes composed of various ligands and receptors, extracellular matrix proteins, intracellular signaling proteins, and the cytoskeleton. The complexes sense changes that occur inside and outside the cells, with Thy-1/CD90 at the core of this extracellular molecular platform. Molecular platforms are scaffold-containing microdomains where key proteins associate to prominently influence cellular processes and behavior. Each component, by itself, is less effective, but when together with various scaffold proteins to form a platform, the components become more specific and efficient to convey the messages. This review article discusses the experimental evidence that supports the role of Thy-1/CD90 as a membrane-associated platform (ThyMAP).

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The RGD motif is involved in CD97/ADGRE5-promoted cell adhesion and viability of HT1080 cells

Cited by 17 publications

References 43 publications

Stoichioproteomics reveal oxygen usage bias, key proteins and pathways in glioma

Stoichioproteomics reveal oxygen usage bias, key proteins and pathways in glioma

CD36 and CD97 in Pancreatic Cancer versus Other Malignancies

Thy-1/CD90 a Bidirectional and Lateral Signaling Scaffold

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