The RFX-Type Transcription Factor DAF-19 Regulates Sensory Neuron Cilium Formation in C. elegans

Swoboda, Peter; Adler, Haskell T.; Thomas, James H.

doi:10.1016/s1097-2765(00)80436-0

Cited by 310 publications

(422 citation statements)

References 57 publications

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“…7 A key promoter element present in a group of genes expressed in ciliated cells, the X-box, 46 was not evident in our comparative analysis of the proximal promoter. An extended search, however, showed that putative X-boxbinding sites were present within 2 kb distance of the ATG start codons in the Pkd1 genes and also in the distal promoters of the Pkd2 genes.…”

Section: Human Gtctcactcttgttgcccaggctggagtgccat---------------------mentioning

confidence: 66%

Common regulatory elements in the polycystic kidney disease 1 and 2 promoter regions

et al. 2005

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The PKD1 and PKD2 genes are mutated in patients with autosomal dominant polycystic kidney disease (ADPKD), a systemic disease, with the formation of renal cysts as main clinical feature. The genes are developmentally regulated and aberrant expression of PKD1 or PKD2 leads to cystogenesis. To date, however, the transcription factors regulating expression of these genes have hardly been studied. To identify conserved putative transcription factor-binding sites, we cloned and characterized the 5 0 -flanking regions of the murine and canine Pkd1 genes and performed a multispecies comparison by including sequences from the human and Fugu rubripes orthologues as well as the Pkd2 promoters from mouse and human. Sequence analysis revealed a variety of conserved putative binding sites for transcription factors and no TATA-box element. Nine elements were conserved in the mammalian Pkd1 promoters: AP2, E2F, EBox, EGRF, ETS, MINI, MZF1, SP1, and ZBP-89. Interestingly, six of these elements were also found in the mammalian Pkd2 promoters. Deletion studies with the mouse Pkd1 promoter showed that a B280 bp fragment is capable of driving luciferase reporter gene expression, whereas reporter constructs containing larger fragments of the Pkd1 promoter showed a lower activity. Furthermore, mutating a potential E2F-binding site within this 280 bp fragment diminished the reporter construct activity, suggesting a role for E2F in regulating cell cycle-dependent expression of the Pkd1 gene. Our data define a functional promoter region for Pkd1 and imply that E2F, EGRF, Ets, MZF1, Sp1, and ZBP-89 are potential key regulators of PKD1 and PKD2 in mammals.

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Section: Human Gtctcactcttgttgcccaggctggagtgccat---------------------mentioning

confidence: 66%

Common regulatory elements in the polycystic kidney disease 1 and 2 promoter regions

et al. 2005

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“…Yet, there are known examples in which cis-regulatory sequences are highly conserved between C. elegans and mammalian promoters. For example, the target genes of DAF-19, an RFX-type TF, contain conserved X boxes of 13-14 bp of imperfect inverted repeats that are similar to the mammalian X boxes (Swoboda et al 2000). Shostak et al (2004) showed that most BSs of the C. elegans intracellular receptor DAF-12 contain conserved sites of the mammalian DR5 motifs (retinoic acid-responsive element) sufficient for transcriptional activation by DAF-12.…”

Section: Genome Research 79mentioning

confidence: 99%

A novel candidate cis-regulatory motif pair in the promoters of germline and oogenesis genes in C. elegans

Linhart

Halperin²,

Darom³

et al. 2011

Genome Res.

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In this study we report on a novel pair of cis-regulatory motifs in promoter sequences of the nematode Caenorhabditis elegans. The motif pair exhibits extraordinary genomic traits: The order and the orientation of the two motifs are highly specific, and the distance between them is almost always one of two frequent distances. In contrast, the sequence between the motifs is variable across occurrences. Thus, the motif pair constitutes a nearly combinatorial sequence configuration. We further show that this module is conserved among, and unique to, the entire Caenorhabditis genus. By analyzing several gene expression data sets, our data suggest that this motif pair may function in germline development, oogenesis, and early embryogenesis. Finally, we verify that the motifs are indeed functional cis-regulatory elements using reporter constructs in transgenic C. elegans.

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“…1C'). Members of the Regulatory Factor X (RFX) family of winged-helix transcription factors regulate ciliogenesis in nematodes, insects, and vertebrates (Swoboda et al, 2000;Dubruille et al, 2002;Bonnafe et al, 2004). A gene knockout of Rfx3 in the mouse resulted in, among others, stunted PNC cilia and laterality defects (Bonnafe et al, 2004).…”

Section: Ciliary Gene Expressionmentioning

confidence: 99%

Three types of cilia including a novel 9+4 axoneme on the notochordal plate of the rabbit embryo

Feistel

Blum

2006

Developmental Dynamics

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Motile monocilia play a pivotal role in left-right axis determination in mouse and zebrafish embryos. Cilia with 9؉0 axonemes localize to the distal indentation of the mouse egg cylinder ("node"), while Kupffer's vesicle cilia in zebrafish show 9؉2 arrangements. Here we studied cilia in a prototype mammalian embryo, the rabbit, which develops via a flat blastodisc. Transcription of ciliary marker genes

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The RFX-Type Transcription Factor DAF-19 Regulates Sensory Neuron Cilium Formation in C. elegans

Cited by 310 publications

References 57 publications

Common regulatory elements in the polycystic kidney disease 1 and 2 promoter regions

Common regulatory elements in the polycystic kidney disease 1 and 2 promoter regions

A novel candidate cis-regulatory motif pair in the promoters of germline and oogenesis genes in C. elegans

Three types of cilia including a novel 9+4 axoneme on the notochordal plate of the rabbit embryo

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