The Revival of Organisms Presumably Killed by Phenol

Flett, Lawrence H.; Haring, Robert C.; Guiteras, Albert F.; Shapiro, Rebecca L.

doi:10.1128/jb.50.5.591-595.1945

Cited by 20 publications

(3 citation statements)

References 8 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…With phenol treated cells, supplementation of nutrient agar with iron, chromium, aluminium and especially magnesium, gave improved revival (Jacobs & Harris, 1961), and the beneficial effect of ferric chloride has been reported also by Harris, Richards & Whitefield (1961). Similar effects with liquid media and phenol treated cells have been reported by Flett, Haring, Guiteras & Shapiro (1945) and Jacobs & Harris (1960), and very marked responses were observed by when cation supplemented media were used with membrane filters. However, Tilley (1948) could not confirm the beneficial effect of ferric chloride for the growth of phenol treated cells.…”

Section: (Ii) Salt Contentsupporting

confidence: 58%

The Influence of the Recovery Medium and the Incubation Temperature on the Survival of Damaged Bacteria

Harris¹

1963

Journal of Applied Bacteriology

View full text Add to dashboard Cite

Section: (Ii) Salt Contentsupporting

confidence: 58%

The Influence of the Recovery Medium and the Incubation Temperature on the Survival of Damaged Bacteria

Harris¹

1963

Journal of Applied Bacteriology

View full text Add to dashboard Cite

“…However, the nephelometric experiments with nutrient broth showed that the beneficial effect obtained could not have been due solely to the action of Norit on the nutrient agar. Further, the suggestion of Flett et al (1945) that Norit in media will adsorb phenol and so increase the probability that a phenol treated cell will grow, cannot be supported, since Jacobs & Harris (1954) found that Norit will remove from broth only small amounts of phenol and, as shown above, it will adsorb only few cells under the same circumstances. Moreover the improved recovery of phenol treated cells was also demonstrated in broth which had been treated with Norit and the Norit then removed.…”

Section: Discussionmentioning

confidence: 97%

The Effect of Environmental Conditions on the Viability and Growth of Bacteria Damaged by Phenols

Jacobs

Harris

1960

Journal of Applied Bacteriology

View full text Add to dashboard Cite

SUMMARY The effects of various environmental conditions on the viability and growth of normal Escherichia coli and Staphylococcus aureus cells, and of cells of these species which had been damaged by exposure to phenol, o‐cresol and p‐chloro‐m‐cresol, have been examined. With normal cells none of the conditions tried had any effect on the initial counts, but sometimes, more often with Staph. aureus, the bulk of growth eventually obtained in broth media was larger, especially when the medium had been made with pyridine extracted peptone, or when it had been treated with 0·1% of Norit charcoal or 0·02% of FeCl3. In contrast, damaged cells of both species were very sensitive to their environment. At room temperature, 0·1% of Norit added to tap water adsorbed more than 80% of the cells and the initial count was increased. Nevertheless the cells died thereafter. Damaged cells inoculated into broth also died, but when Norit was present both the rate and the extent of death were markedly reduced, though few cells were adsorbed. The addition of FeCl3 (0·02 or 0·03%) had no effect. At 37° damaged cells died faster than at room temperature, but the protective effect of Norit was still evident. In nephelometric studies of cultures growing in Norit treated broth the times to turbidity were shorter than those of control cultures in untreated broth, but broth treated with FeCl3 often gave longer times than the controls. In a comparison of broths prepared with peptone and/or Lab‐Lemco which had been extracted with pyridine to remove suspected toxic substances, the initial counts of treated cells were highest when only the peptone had been extracted. With media containing extracted Lab‐Lemco, or with both ingredients extracted, the counts were smaller but were still generally higher than in normal broth. The results of nephelometric studies were consonant with these findings: broths prepared with extracted ingredients generally gave shorter turbidity times than normal broth, especially with E. coli. The addition to broth of cations, especially Mg2+ (10–3 M), favoured the survival of damaged E. coli but did not influence that of Staph. aureus. But with both species, five times that concentration of Mg2+ was as effective as 0·1% of Norit, which was shown to adsorb lead, tin, copper, zinc, silver and cobalt from broth. Temperatures above 37° were unfavourable to the survival of damaged cells of both species, 44° often being completely inhibitory for Staph. aureus, but this effect was not due to traces of phenol in the inoculum. At 25° damaged E. coli recovered less well than at 30° and 37°, but damaged Staph. aureus did better at 25° and 30° than at 37°.

show abstract

“…56 added 0.1 per cent norite. The importance of using an antidote in disinfection studies on phenol has been discussed by Flett, Haring, Guiteras, and Shapiro (1945). Plate count results were read after 3 days' incubation at 37 C, and survivor curves were plotted (figure 5).…”

mentioning

confidence: 99%

Studies on Heat Resistance

1948

View full text Add to dashboard Cite

The literature pertaining to studies on the thermal resistance of bacterial spores reveals few successful attempts to increase the resistance of these forms by selective cultivation of survivors of heated spore suspensions. Among the workers presenting evidence for such an increase are Bigelow and Esty (1920), Magoon (1926), 0. B. Williams (1929), F. T. Williams (1936), and Davis and Williams (1948). The authors are unaware of any published data concerning a correlation of the increased thermal resistance of bacterial spores with increased resistance to chemical agents. From this standpoint it appeared desirable to ascertain whether the spores of a culture whose resistance to heat had been enhanced by repeated subculturing of survivors would exhibit a greater resistance to certain commonly employed bacteriostatic and bactericidal agents than do the spores of the parent culture from which these resistant forms were derived. Comparative studies are presented on the bacteriostatic action of mercuric chloride, gentian violet, and streptomycin, and the sporicidal action of phenol and iodine for a stock strain of Bacillus globigii and a heat-resistant variant developed from it.

show abstract

The Revival of Organisms Presumably Killed by Phenol

Cited by 20 publications

References 8 publications

The Influence of the Recovery Medium and the Incubation Temperature on the Survival of Damaged Bacteria

The Influence of the Recovery Medium and the Incubation Temperature on the Survival of Damaged Bacteria

The Effect of Environmental Conditions on the Viability and Growth of Bacteria Damaged by Phenols

Studies on Heat Resistance

Contact Info

Product

Resources

About