The retrospective identification and molecular epidemiology of porcine circovirus type 3 (
            <scp>PCV</scp>
            3) in swine in Thailand from 2006 to 2017

Sukmak, Manakorn; Thanantong, Narut; Poolperm, Pariwat; Boonsoongnern, Alongkot; Ratanavanichrojn, Nattavut; Jirawattanapong, Pichai; Woonwong, Yonlayong; Soda, Nantana; Kaminsonsakul, Tanyanant; Phuttapatimok, Sahathat; Wajjwalku, Worawidh

doi:10.1111/tbed.13057

Cited by 37 publications

(24 citation statements)

References 19 publications

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“…On the other hand, other studies have confirmed that there is no significant genetic difference in PCV3 strains in different regions. Previous studies have shown that the high genetic stability of pigorigin PCV3 over the past 20 years (Sukmak et al, 2019;Sun et al, 2018). In the present study, the dog-origin PCV3 strain in this study share hight nucleic acid similarity (> 95%) with reference sequences of pig-origin PCV3.…”

Section: Discussionsupporting

confidence: 61%

An epidemiological investigation of porcine circovirus 3 infection in dogs in the Guangxi Province from 2015 to 2017, China

et al. 2019

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A B S T R A C TPorcine circovirus type 3 (PCV3) is an emerging circovirus species associated with several diseases. The study aimed to investigate the frequency of porcine circovirus 3 (PCV3) and its coinfection with canine parvovirus type 2 (CPV-2) in dogs in the Guangxi province from 2015 to 2017, China, and to examine the genome diversity of PCV3. Using polymerase chain reaction (PCR) amplification and sequencing, 96 of 406 (23.6%)samples were positive for PCV3, 38 out of 406 (9.4%) samples were coinfected with both PCV3 and CPV-2. The CPV-positive rate was significantly higher in the PCV3-positive samples than in the non-PCV3 samples, and the difference was extremely significant (P < 0.01). The complete genome (n=4) and ten capsid genes (n=10) of PCV3 were sequenced. Multiple sequence alignment results showed that these sequences shared 98.5-100% nucleotide similarity with the reference genome sequence and 97.5-100% nucleotide similarity with the reference capsid gene sequence. PCV3 was classified into two different genotypes, according to phylogenetic analysis based on the whole genome. These strains were clustered in PCV3a, showing a close relationship with PCV3-US/SD2016. Surprisingly, we separately analyzed these PCV3 strains from the Guangxi province and found that the dog and pig PCV3 are from different branches. In summary, this was the first seroprevalence and genetic investigation of PCV3 in dogs in the Guangxi province, China, and the first complete genome PCV3 from dogs obtained in the world. The results provide insights into the epidemiology and pathogenesis of this important virus.

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Section: Discussionsupporting

confidence: 61%

An epidemiological investigation of porcine circovirus 3 infection in dogs in the Guangxi Province from 2015 to 2017, China

et al. 2019

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“…PCV3 is a novel porcine viral pathogen that was first recognized in commercial pig farms in the USA in 2016, and then it was reported in dozens of counties, including China, South Korea, Thailand, Italy, Poland, and so on [5,6,17,24,25]. Up to date, PCV3 has been detected in pigs within different clinical presentations, including respiratory syndrome, reproductive failure, multisystemic inflammation, and gastrointestinal and neurological disorders [4,21,26].…”

Section: Discussionmentioning

confidence: 99%

“…In 2016, a new species of PCV was first recognized in pigs from a case of PDNS and reproductive failure in the USA via next generation sequencing (NGS) technology, and designated as porcine circovirus 3 (PCV3) [4]. Subsequently, PCV3 was found to be widely distributed in pigs around the world, and up to date, it has been reported in at least 14 countries, including the USA, Japan, China, South Korea, Russia, Brazil, Thailand, Poland, Sweden, Italy, Germany, Denmark, Spain, and the UK [5][6][7][8][9][10][11][12]. In China, PCV3 was first reported in Liaoning Province, Jiangxi Province and Chongqing city in 2017 [13].…”

Section: Introductionmentioning

confidence: 99%

Porcine Circovirus Type 3 in Pig Farms Experiencing Diarrhea in Jiangxi, China: Prevalence, Genome Sequence and Pathogenicity

Zhang

Yuan

et al. 2020

Animals

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Porcine circovirus 3 (PCV3) infections have been reported in different clinical presentations. However, the prevalence and pathogenicity of PCV3 associated with diarrhea in piglets have been limited. Herein, we present an investigation and genome analyses of PCV3 in piglets experiencing diarrhea, and observed clinical signs, gross lesions, and histological changes in pigs negative for all known pathogens associated with diarrhea but positive for PCV3 alone. Among the feces (n = 141) tested, 16.31% (23/141) were positive for PCV3. Of which, 27.28% (15/55) and 14.29% (5/35) were present in diarrheal samples from suckling and weaned piglets, respectively. Moderate to severe atrophic villi was confined in duodenum, jejunum, and ileum, and significantly decreased average heights of villi, and the depths of crypt were observed in PCV3-infected piglets. The complete genome of a representative strain of PCV3, designated as JX/CH/2018, was determined. Multialignment analysis indicated that JX/CH/2018 had 97.7–99.7% nucleotide identity at the complete genome level, and 97.2–100% at the amino acid level of the capsid protein when compared with reference PCV3 strains. Phylogenetic analysis showed that the PCV3 strain identified in this study belonged to PCV3a lineage. The present study demonstrated that PCV3 is a common virus in diarrheal suckling and weaned piglets.

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“…A successful reproduction of PDNS-like clinical disease following experimental inoculation of 4-and 8-week-old specific-pathogen-free piglets with infectious PCV3 DNA clone has been demonstrated, thus implying that PCV3 may have a direct role in disease process [18]. Considering the presence of PCV3 in increasing number of countries including Thailand [17,19] and U.S. [4,13] with whom Malaysia shares trade and geographical relationship; and that PCV3 is associated with several clinical presentations that affect productivity, molecular prevalence of PCV3 in Malaysia and molecular characteristics of Malaysian PCV3 strains reported in this study may contribute practical knowledge to the Malaysian swine farming industry.…”

Section: Introductionmentioning

confidence: 99%

First molecular detection and complete sequence analysis of porcine circovirus type 3 (PCV3) in Peninsular Malaysia

et al. 2020

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Porcine circovirus type 3 (PCV3) is a newly emerging virus in the swine industry, first reported recently in 2016. PCV3 assembles into a 2000 bp circular genome; slightly larger than PCV1 (1758-1760 bp), PCV2 (1766-1769 bp) and PCV4 (1770 bp). Apart from being associated with porcine dermatitis and nephropathy syndrome (PDNS), PCV3 has been isolated from pigs with clinical signs of reproductive failures, myocarditis, porcine respiratory disease complex (PRDC) and neurologic disease. Given that PCV3 is increasingly reported in countries including Thailand and U.S. with whom Malaysia shares trade and geographical relationship; and that PCV3 is associated with several clinical presentations that affect productivity, there is a need to study the presence and molecular characteristics of PCV3 in Malaysian swine farms. Twenty-four commercial swine farms, three abattoirs and retail shops in Peninsular Malaysia were sampled using convenience sampling method. A total of 281 samples from 141 pigs, including 49 lung archive samples were tested for PCV3 by conventional PCR. Twenty-eight lung samples from wild boar population in Peninsular Malaysia were also included. Nucleotide sequences were analyzed for maximum likelihood phylogeny relationship and pairwise distances. Results revealed that PCV3 is present in Peninsular Malaysia at a molecular prevalence of 17.02%, with inguinal lymph nodes and lungs showing the highest molecular detection rates of 81.82% and 71.43% respectively. Despite wide reports of PCV3 in healthy animals and wild boars, no positive samples were detected in clinically healthy finishers and wild boar population of this study. PCV3 strain A1 and A2 were present in Malaysia, and Malaysian PCV3 strains were found to be phylogenetically related to Spanish, U.S. and Mexico strains.

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The retrospective identification and molecular epidemiology of porcine circovirus type 3 ( PCV 3) in swine in Thailand from 2006 to 2017

Cited by 37 publications

References 19 publications

An epidemiological investigation of porcine circovirus 3 infection in dogs in the Guangxi Province from 2015 to 2017, China

An epidemiological investigation of porcine circovirus 3 infection in dogs in the Guangxi Province from 2015 to 2017, China

Porcine Circovirus Type 3 in Pig Farms Experiencing Diarrhea in Jiangxi, China: Prevalence, Genome Sequence and Pathogenicity

First molecular detection and complete sequence analysis of porcine circovirus type 3 (PCV3) in Peninsular Malaysia

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