The retinoid metabolism of Gammarus fossarum is disrupted by exogenous all-trans retinoic acid, citral, and methoprene but not by the technical formulation of glyphosate

Gauthier, Maxime; Danièle, Gaëlle; Giroud, Barbara; Lafay, Florent; Vulliet, Emmanuelle; Jumarie, Catherine; Garric, Jeanne; Boily, Monique; Geffard, Olivier

doi:10.1016/j.ecoenv.2023.114602

Cited by 3 publications

(1 citation statement)

References 26 publications

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“…The retinoid system has been explored scarcely in crustaceans (Liñán-Cabello et al, 2002). However, some retinoids (oxidated forms of retinoate, retinoate isomers, and retinaldehyde isomers) were quantified in G. fossarum and were shown to fluctuate during the reproductive cycle in females and their levels were affected by methoprene, a juvenile hormone analog (Gauthier et al, 2023). Another over-represented KEGG pathway in the gammarid caeca was the glutathione metabolism with an ER of about 12% (FDR<2.2 e -16) (Figure 6D, Table S6).…”

Section: Organ-specific Metabolism Through Proteomic Profilingmentioning

confidence: 99%

Proteogenomic reconstruction of organ-specific metabolic networks in an environmental sentinel species, the amphipodGammarus fossarum

Koenig,

Baa-Puyoulet,

Lafont

et al. 2024

Preprint

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Metabolic pathways are targets of environmental contaminants underlying a large variability of toxic effects throughout biodiversity. However, the systematic reconstruction of metabolic pathways remains limited in environmental sentinel species due to the lack of available genomic data in many taxa of animal diversity. In order to improve the knowledge of the metabolism of sentinel species, in this study we used a multi-omics approach to reconstruct the most comprehensive map of metabolic pathways for a crustacean model in biomonitoring, the amphipod Gammarus fossarum. We revisited the assembly of RNA-seq data by de novo approaches drastically reducing RNA contaminants and transcript redundancy. We also acquired extensive mass spectrometry shotgun proteomic data on several organs from G. fossarum males and females to identify organ-specific metabolic profiles. The G. fossarum metabolic pathway reconstruction (available through the metabolic database GamfoCyc) was performed by adapting the genomic tool CycADS and we identified 377 pathways representing 7,630 annotated enzymes, 2,610 enzymatic reactions and the expression of 858 enzymes was experimentally validated by proteomics. Our analysis shows organ-specific metabolic profiles, such as an elevated abundance in enzymes involved in ATP biosynthesis and fatty acid beta-oxidation indicative of the high-energy requirement of the gills, or the key anabolic and detoxification role of the hepatopancreatic caeca, as exemplified by the specific expression of the retinoid biosynthetic pathways and glutathione synthesis. In conclusion, the multi-omics data integration performed in this study provides new resources to investigate metabolic processes in crustacean amphipods and their role in mediating the effects of environmental contaminant exposures in sentinel species.

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Section: Organ-specific Metabolism Through Proteomic Profilingmentioning

confidence: 99%

Proteogenomic reconstruction of organ-specific metabolic networks in an environmental sentinel species, the amphipodGammarus fossarum

Koenig,

Baa-Puyoulet,

Lafont

et al. 2024

Preprint

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Disruption of oogenesis and molting by methoprene and glyphosate in Gammarus fossarum: involvement of retinoic acid?

Gauthier

Defrance

Jumarie

et al. 2023

Environ Sci Pollut Res

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In the last decade, the freshwater amphipod Gammarus fossarum proved to be a promising sentinel species in active biomonitoring programs to assess the effects of environmental contamination on nontarget organisms. In many vertebrates, xenobiotics cause deregulation of the retinoids (RETs) metabolism implicated in many crucial biological functions. The retinoid system, known in the crustaceans, is yet to be explored in G. fossarum. Therefore, we studied the implication of all-trans retinoic acid (atRA) in the reproduction (embryo, oocyte, and juvenile production) and development (success and delay of moulting) by exposing G. fossarum females to atRA and citral (CIT), a known inhibitor of RA synthesis to simulate an excess or a de ciency of RA, respectively. In parallel, we monitored the effects of methoprene (MET) and glyphosate (GLY), two pesticides suspected to interfere with atRA metabolism and signalling and frequently found in water systems. After 14 days of exposure, atRA, CIT, and MET reduced the number of oocytes, whereas only MET caused a reduced number of embryos. After 44 days, MET and GLY showed a tendency to decrease juvenile production. The duration of the moulting cycle increased following the exposures to atRA and MET, while the treatment with CIT caused a typical endocrine disruptive inverted Ushaped curve. The exposure to GLY led to increased duration of the moulting cycle at the lowest concentrations and lowered moulting success at the highest concentration tested. This study highlights for the rst time the implication of RA in the oogenesis and moulting of G. fossarum and suggests that it may be a potential mediator of MET-induced effects on these processes. This study adds to the comprehension of the reproductive and developmental control in G. fossarum and opens new research avenues to study the effects of xenobiotics on the RET system in this sentinel species. Ultimately, our study will drive the development of RET-based biomarkers for non-target aquatic invertebrates exposed to xenobiotics.

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Proteogenomic reconstruction of organ-specific metabolic networks in an environmental sentinel species, the amphipod Gammarus fossarum

Koenig,

Baa-Puyoulet,

Lafont

et al. 2024

Comparative Biochemistry and Physiology Part D: Genomics and Pr

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The retinoid metabolism of Gammarus fossarum is disrupted by exogenous all-trans retinoic acid, citral, and methoprene but not by the technical formulation of glyphosate

Cited by 3 publications

References 26 publications

Proteogenomic reconstruction of organ-specific metabolic networks in an environmental sentinel species, the amphipodGammarus fossarum

Proteogenomic reconstruction of organ-specific metabolic networks in an environmental sentinel species, the amphipodGammarus fossarum

Disruption of oogenesis and molting by methoprene and glyphosate in Gammarus fossarum: involvement of retinoic acid?

Proteogenomic reconstruction of organ-specific metabolic networks in an environmental sentinel species, the amphipod Gammarus fossarum

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