The retention behaviour of conjugated bile acids in reversed phase high performance liquid chromatography

Abstract

The retention behaviour of conjugated bile acids has been studied in a reversed phase high performance liquid chromatographic (RP-HPLC) system by using the mixture of methanol and aqueous phosphate buffer as the mobile phase. The retentions of the conjugates in RP-HPLC have been found to be mainly controlled by the glycine and taurine groups. The selectivity between five different glycine and taurine conjugated bile acids is a constant in RP-HPLC. This selectivity has been used for peak identification in the practical separation of conjugated bile acids.

HPLC-Fluorescence Determination of Individual Free and Conjugated Bile Acids in Human Serum

“…Therefore, the separation and quantification of BA are very important for the diagnosis, prognosis and clarification of pathophysiology, but present difficulties because of their low serum levels. High-performance liquid chromatography (HPLC) is well recognized as an effective tool for the analysis of unconjugated and conjugated BA (Coca et al, 1994;Goldsmith et al, 1994;Goto, 1990;Nambara and Goto, 1988;Nichols et al, 1993;Zhang et al, 1992;Zhao et al, 1993). These compounds, however, do not present significant UV absorption, fluorescent or electrochemical properties suitable for their sensitive detection.…”