The Results of Hemoglobin Variant Analysis in Patients Revealing Microcytic Erythrocytosis on Complete Blood Count

Joneja, Upasana; Gulati, Gene; Florea, Alina Dulau; Gong, Jerald Z.

doi:10.1093/labmed/lmx071

Cited by 5 publications

(8 citation statements)

References 16 publications

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“…Higher HGB and RCC in patients with alpha thalassemia is due to a reduction in the rate of hemolysis 31 and increased red cell precursor mitosis, smaller red cells (low MCV) and relative erythrocytosis for the degree of anaemia. 32 Our finding corroborates the previous report about high haemoglobin in patients with α-thalassemia, which is related to the number of alpha deletions. 33 White blood cell count (WBC) and platelet count (PCT) have been documented to be higher in patients with SCD compared with their healthy counterparts.…”

Section: Haemoglobins F and A2 In The Study Populationsupporting

confidence: 92%

THE EFFECT OF ALPHA THALASSEMIA, HBF and HBC ON HAEMATOLOGICAL PARAMETERS OF SICKLE CELL DISEASE PATIENTS IN IBADAN, NIGERIA.

Fasola

Babalola

Brown

et al. 2022

Mediterr J Hematol Infect Dis

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Background: Sickle cell disease is a protean disease with limited data on the phenotypic and genetic variants in Nigeria. This study was conducted to provide baseline data on these variants by characterizing the existing forms of sickle cell disease and correlating these with basic hematological parameters. Methods: Adult and pediatric patients with SCD were recruited from a tertiary health centre in Nigeria. Patients were age and sex matched with healthy controls. Blood samples were obtained for Full Blood Count, phenotyping by High Performance Liquid Chromatography and genotyping for alpha thalassemia by multiplex gap polymerase chain reaction. Data analysis was done using IBM SPSS statistics version 23. Results: A total of 130 patients with sickle cell disease and 117 controls were studied. Alpha thalassemia in the study population was due to a 3.7kb deletion in the alpha globin gene cluster at a prevalence of 45.4% in the patients and 47% in controls. The prevalence of the various existing forms of SCD genotype was: Homozygous S without alpha gene deletion (HbSS)- 39.2%; HbSC - 10.8%; HbSα+1- 35.4%; HbSα+2 - 6.9% and HbSF- 7.7%. HbA2 was significantly elevated in individuals with two alpha gene deletions (HbSα+2). HbF and HbA2 were negatively correlated with each other (r= -0.587, p < 0.001). Individuals with the HbSC genotype followed by HbSα+2 had the best hematological parameters. Conclusions: Hematological parameters varied with hemoglobin genotype. The C hemoglobin and homozygous alpha thalassemia deletion had better ameliorating effect on SCD hematological parameters than the F hemoglobin in this population.

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Section: Haemoglobins F and A2 In The Study Populationsupporting

confidence: 92%

THE EFFECT OF ALPHA THALASSEMIA, HBF and HBC ON HAEMATOLOGICAL PARAMETERS OF SICKLE CELL DISEASE PATIENTS IN IBADAN, NIGERIA.

Fasola

Babalola

Brown

et al. 2022

Mediterr J Hematol Infect Dis

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“…The morphological manifestation within the erythrocytes for its size and chromium has long been evaluated for underlying etiology. The appreciation for this morphological alteration is always manual by microscopy of a stained blood film and the electronic cell counters in modern hematology laboratories [2].…”

Section: Introductionmentioning

confidence: 99%

“…The treatment of MCHC is the iron replacement therapy if the iron deficiency is suspected as its cause. However, the therapeutic iron can produce harmfuleffects if the underlying pathology for MCHC is otherwise like, betathalassemia trait, sickle cell disease, or the other hemoglobinopathies [1][2][3][4][5].…”

Section: Introductionmentioning

confidence: 99%

A Study Protocol for Evaluation of Microcytic Hypochromic Anemia by High-Performance Liquid Chromatography (HPLC)

Kharche

Bhake

Vagha

2021

JPRI

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Background: One of the most regularassessments done in clinical practice is anemia. Different levels classify the anemiasbased onthe numerical value of hemoglobin, alteration in the morphology and chromium, underlying etiologies, red cell volumetric parameters and functionally depending on pathophysiologic processes of anemia. Microcytic hypochromic anemia (MCHC) has different underlying causes, including iron deficiency anemia, beta-thalassemia trait, and hemoglobinopathies. Before planning the treatment, assessing the hemoglobin for its variants and the detection of abnormal hemoglobinis mandatory. High-performance liquid chromatography (HPLC) has proved to be significant for the evaluation of MCHC because of its superior separation and quantification analytical powers.The present study has been undertakingthat anemia is the most prevailing and commonly treated clinical state managed by numerous nutritional supplements without much being done to know the underlying etiology or without evaluation of hemoglobin for its abnormalities. Methods: This will bean observational (Prospective and Retrospective) study. Blood samples of 100 patients will be evaluated by HPLCBio-Rad variant,and various hemoglobin patterns; associate dhemoglobinopathies will be evaluated. Results: The observations will be made according to the objectives and tabulated, which will be subjected to statistical tests for their significance and conclusions. Conclusion: This study would contribute to understanding and knowing the etiology of MCHC in the population that our tertiary care hospitals serve.

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“…IDA is usually diagnosed by an iron metabolism test, whereas early diagnosis of TT is solely based on hemoglobin analysis (HbA 2 and abnormal Hb). 2,3 DNA mutation analysis is now used to boost the accuracy of TT diagnosis, especially in cases of mild to moderate TT carriers. 4 In addition, implementation of DNA mutation analyses in hospitals with limited healthcare resources can be really challenging, especially primary hospitals, due to its demanding technical conditions, complicated operation, time consuming nature, and extremely high cost.…”

Section: Introductionmentioning

confidence: 99%

“…IDA is usually diagnosed by an iron metabolism test, whereas early diagnosis of TT is solely based on hemoglobin analysis (HbA 2 and abnormal Hb) 2,3 . DNA mutation analysis is now used to boost the accuracy of TT diagnosis, especially in cases of mild to moderate TT carriers 4 .…”

Section: Introductionmentioning

confidence: 99%

Application of HbA₂ levels and red cell indices‐based new model in the differentiation of thalassemia traits from iron deficiency in hypochromic microcytic anemia Cases

Zhou

Zhang

Wang

et al. 2020

Int J Lab Hematology

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Introduction Thalassemia traits and iron deficiency anemia are the most common types of hypochromic microcytic anemia with similar clinical and laboratory features. It is vital to establish a new screening model based on HbA2 levels and red cell indices for the differentiation of TT from IDA in hypochromic microcytic anemia cases. Method The data comprised of the red blood cell indices and HbA2 prenatal diagnostic test results of 810 individuals who were identified to conform to the following criteria: MCV < 80 fl or MCH < 26 pg. We launched a new model consisting mainly of significative red cell indices and HbA2 levels, as well as proposing cutoff values by using decision trees and logistic regression analyses. Next, we evaluated our new method by comparing the sensitivity, specificity, positive, and negative predictive values with those of the previous formulas. Results We put forward a new model and compared it with 5 efficient formulas. The new model exhibited the highest accuracy (0.918), with its sensitivity and specificity calculated as 0.917 and 0.921, respectively. Our new model's Youden index was 0.838, which is higher than the other formulas' Youden indices. Conclusions The new screening model, based on HbA2 levels and red cell indices, is suitable for the screening of thalassemia patients in the hypochromic microcytic anemia group and has the best efficiency in distinguishing TT and IDA.

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The Results of Hemoglobin Variant Analysis in Patients Revealing Microcytic Erythrocytosis on Complete Blood Count

Abstract: Hemoglobin variant analysis provided a very high positive yield in determining the etiology of microcytic erythrocytosis.

Cited by 5 publications

References 16 publications

THE EFFECT OF ALPHA THALASSEMIA, HBF and HBC ON HAEMATOLOGICAL PARAMETERS OF SICKLE CELL DISEASE PATIENTS IN IBADAN, NIGERIA.

THE EFFECT OF ALPHA THALASSEMIA, HBF and HBC ON HAEMATOLOGICAL PARAMETERS OF SICKLE CELL DISEASE PATIENTS IN IBADAN, NIGERIA.

A Study Protocol for Evaluation of Microcytic Hypochromic Anemia by High-Performance Liquid Chromatography (HPLC)

Application of HbA₂ levels and red cell indices‐based new model in the differentiation of thalassemia traits from iron deficiency in hypochromic microcytic anemia Cases

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The Results of Hemoglobin Variant Analysis in Patients Revealing Microcytic Erythrocytosis on Complete Blood Count

Abstract: Hemoglobin variant analysis provided a very high positive yield in determining the etiology of microcytic erythrocytosis.

Cited by 5 publications

References 16 publications

THE EFFECT OF ALPHA THALASSEMIA, HBF and HBC ON HAEMATOLOGICAL PARAMETERS OF SICKLE CELL DISEASE PATIENTS IN IBADAN, NIGERIA.

THE EFFECT OF ALPHA THALASSEMIA, HBF and HBC ON HAEMATOLOGICAL PARAMETERS OF SICKLE CELL DISEASE PATIENTS IN IBADAN, NIGERIA.

A Study Protocol for Evaluation of Microcytic Hypochromic Anemia by High-Performance Liquid Chromatography (HPLC)

Application of HbA2 levels and red cell indices‐based new model in the differentiation of thalassemia traits from iron deficiency in hypochromic microcytic anemia Cases

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Application of HbA₂ levels and red cell indices‐based new model in the differentiation of thalassemia traits from iron deficiency in hypochromic microcytic anemia Cases