The Responses of the Lipoxygenase Gene Family to Salt and Drought Stress in Foxtail Millet (Setaria italica)

Abstract

Plant lipoxygenases (LOXs), a kind of non-heme iron-containing dioxygenases, participate plant physiological activities (especially in response to biotic and abiotic stresses) through oxidizing various lipids. However, there was few investigations on LOXs in foxtail millet (Setaria italica). In this study, we identified the LOX gene family in foxtail millet, and divided the total 12 members into three sub-families on the basis of their phylogenetic relationships. Under salt and drought stress, LOX genes showed different expression patterns. Among them, only SiLOX7 showed up-regulated expression in Yugu1 (YG1) and Qinhuang2 (QH2), two stress-tolerant varieties, indicating that SiLOX7 may play an important role in responses to abiotic stress. Our research provides a basis for further investigation of the role of LOX genes in the adaptation to abiotic stresses and other possible biological functions in foxtail millet.

Comparative transcriptomics reveals key genes contributing to the differences in drought tolerance among three cultivars of foxtail millet (Setaria italica)

“…(Seita.4G273900) were also detected to be involved in the salt stress response of foxtail millet in previous studies (Puranik et al, 2011;Liu et al, 2016;Zhang et al, 2021). In addition to foxtail millet, these genes were also identified in plant abiotic stress responses in other species.…”

“…This study identified 46 genes whose expression levels changed regularly in JiguX/Jigu, JinguX/Jingu, and LongguX/Longgu (Table 2). In previous studies, Seita.2G141500 (Zhang et al, 2022), Seita.1G239600, Seita.5G022000, Seita.9G379000 (Shi et al, 2018), Seita.1G326200, Seita.5G294000, Seita.7G045200 (Qin et al, 2020), Seita.1G349800 (Zhao et al, 2021), Seita.5G393800 (Liu et al, 2016), and Seita.7G113700 (Zhang et al, 2021) have been identified to play an important role in the drought process of foxtail millet. In addition, SiBZIP12 (Seita.5G393800), SiPP2C50 (Seita.5G294000), SiSAPK1…”

“…Lipoxygenase catalyzes the production of oxytocin and plays a key role in the whole growth period of plants (such as seed germination, plant growth, and stress resistance). A previous study by Zhang et al (2021) showed that the transcription level of Seita.7G113700…”

Cannabis sativa oxylipin biosynthesis: Genome-wide characterization of lipoxygenase, allene oxide synthase, allene oxide cyclase, hydroperoxide lyase, and 12-oxo-phytodienoic acid reductase gene families

“…This study adds Cannabaceae to the collection of plant families with described LOX gene families, a group which so far includes Aracae: duckweed (102); Actinidiaceae: kiwi (103); Brassicaceae: Arabidopsis thaliana (104–106), radish (107), and turnip (108); Caricaceae: papaya (106); Cucurbitaceae: cucumber (109, 110), melon (111), and watermelon (112); Fabaceae: Medicago truncatula, peanut (113), and soybean (114); Malvaceae: cotton (115) Muscacea: banana (116); Poaceae: maize (117), rice (105), Setaria italica (118), sorghum (119); Polygonaceae: buckwheat (120); Rhamnaceae: Jujubie (121); Rosaceae: apple (122), peach (123), pear (124); Salicaceae: poplar (125); Solanaceae: pepper (126) and tomato (127, 128); Theaceae: Camellia sinensis (129); and Vitaceae: grape (130).…”

Jasmonate-Dependent Response of the Flower Abscission Zone Cells to Drought in Yellow Lupine

“…Increased mRNA levels of the PLD gene and activity of PLD was observed in A. thaliana [27], sweet orange [28], Craterostigma plantagineum [29], Petunia [7], Vigna radiata [30], Z. mays [31], and Arachis hypogaea [32] under drought. In turn, accumulation of LOX mRNA evoked by water deficit has been demonstrated in Capsicum annuum L. [33], P. vulgaris [34], Panax ginseng [35], Diospyros kaki [36], Setaria italica [37], Cucumis melo [38], Solanum lycopersicum [39], Agrostis stolonifera [40], and Triticum aestivum [41]. LOX-encoding lipoxygenases catalyze the conversion of α-linolenic acid to (13S)-hydroperoxy-octadecatrienoic acid (13-HPOT), which is further subjected to processes of oxidation, cyclization, and acyl chain shortening, to finally form JA [42,43].…”