The response surface methodology for optimization of tyrosinase immobilization onto electrospun polycaprolactone–chitosan fibers for use in bisphenol A removal

Abstract

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Electrochemical Detection of Bisphenol A by Tyrosinase Immobilized on Electrospun Nanofibers Decorated with Gold Nanoparticles

“…Furthermore, the immobilization of the Tyr enzyme proved effective for sensitive detection of BPA, as demonstrated with the lower LOD of PA6/PAH@AuNPs/Tyr than with other enzyme-free nanofiber-based electrochemical sensors [36,37]. In addition, our work opens the possibility for developing multifunctional nanofibers as tyrosinase-based nanofibrous membranes have been applied for BPA removal [34]. The possible interference from potential interferents in the detection of BPA was investigated, and the results are present in Table 2.…”

“…Note in Figure 4 that the response curve tended to reach a plateau for concentrations above 20 μM, indicating typical Michaelis-Menten enzyme kinetics. The apparent Michaelis-Menten constant (Km) was 0.34 mM, lower than the Km of the free enzyme (1.46 mM) reported for the BPA substrate [34]. This low Km indicates that Tyr re- Chronoamperometry was used to investigate the performance of the biosensor in determining BPA.…”

Current Status and Future Perspectives of Supports and Protocols for Enzyme Immobilization

“…The electrified nanofibers have attracted the attention of enzyme engineering and biocatalysis, being considered a potential tool because of their numerous advantages: high surface area, multiple fixation points to the support, high porosity, interconnectivity, high thermal resistance, pH stability, and several solvents [216,220,222,223,227]. The process of immobilizing enzymes in electrified fibers usually promotes the retention and improvement of biological catalytic activity and allows for the easy separation of the enzyme from the proposed reaction environment [216,223,224,228,229].…”

“…Note that the surface fixation process refers to the physical adsorption or fixation of enzymes in pure or functionalized nanofibrous supports chemically or physically, and encapsulation means electrospinning of the enzyme and the polymer mixture [219,222,223,228,229].…”

Novel Chemical Architectures Based on Beta-Cyclodextrin Derivatives Covalently Attached on Polymer Spheres

“…Different methods for BPA removal were directed to biological treatment, membrane adsorption processes, or advanced oxidation [ 1 ]. The biological treatment involves the degradation of BPA by immobilization of enzymes such as oxidoreductase or polyphenol oxidases [ 8 ] that are capable of oxidizing organic pollutants, such as phenols, organic dyes, or drugs. Advanced oxidation is a method that removes BPA by the generation of highly reactive radicals or the application of photocatalytic treatment to break down the molecules of BPA into less harmful compounds from water, sediment, and soil [ 1 , 9 , 10 , 11 ].…”