Laboratory mice infected with a Trypanosoma congolense isolate of water-buffalo origin did not respond when treated with diminazene aceturate (Berenil, Hoechst, West Germany) at 3.5 mg/kg and 7 mg/kg body weight (b.wt.) and also isometamidium chloride (Samorin, May & Baker, Dagenham, England) at doses less than 3 mg/kg b.wt. Effective cure was achieved with isometamidium and diminazene at 3 mg/kg and 14 mg/kg b.wt. respectively; doses in excess of those recommended for use in the field to treat animal trypanosomiasis by the manufacturers. Irregular use of the two drugs on infected waterbuffaloes while improperly restrained may be one of the factors responsible for the dual drug resistant T. congolense isolate emerging.