The Response of Prostatic Fluid pH in Inflammation

Blacklock, N. J.; Beavis, J. P.

doi:10.1111/j.1464-410x.1974.tb03853.x

Cited by 79 publications

(34 citation statements)

References 7 publications

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“…The data are supported by the observation that the pH of the prostatic fluid is acidic (6.1-6.6) due to the secretion of the high amount of citrate, which would favor zinc reabsorption via ZIP2 at the apical surface of the prostate epithelial cells under physiological conditions. [18][19][20] As was reported previously for other ZIP transporters (ZIP8 and ZIP14), the cadmium uptake via hZIP2 was decreased by copper and zinc through competitive inhibition experiments, suggesting that these divalent cations are transported by hZIP2 as well. 21,22 To further support that our assay indeed measures hZIP2 activity, we tested the effect of extracellular zinc on the cadmium influx via hZIP2.…”

Section: +supporting

confidence: 59%

Development of the First Fluorescence Screening Assay for the SLC39A2 Zinc Transporter

et al. 2014

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Zinc is an essential micronutrient that is crucial for many vital cellular functions such as DNA and protein synthesis, metabolism, and intracellular signaling. Therefore, the intracellular zinc concentration is tightly regulated by zinc transporters and zinc-binding proteins. The members of the SCL39 transporter family transport zinc into the cytosol. The SLC39A2 (hZIP2) protein is highly expressed in prostate epithelial cells and was found to be involved in prostate cancer development. Thus far, there is no specific modulator available for the SLC39 transporters. The aim of this study was to develop a screening assay for compound screening targeting hZIP2. Employing the pIRES2-DsRed Express 2 bicistronic vector, we detected human ZIP2 expression at the plasma membrane in transiently transfected HEK293 cells. Using the FLIPR Tetra fluorescence plate reader, we demonstrated that ZIP2 transports Cd 2+ with an apparent K m value of 53.96 nM at an extracellular pH of 6.5. The cadmium influx via hZIP2 was inhibited by zinc in a competitive manner. We found that hZIP2 activity can be measured using cadmium in the range of 0.1 to 10 µM with our assay. In summary, for the first time we developed an assay for human ZIP2 that can be adapted to other zinc transporters.

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Section: +supporting

confidence: 59%

Development of the First Fluorescence Screening Assay for the SLC39A2 Zinc Transporter

et al. 2014

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“…Our study of 60 men in which semen culture was positive showed no differences between antibiotic treatments and placebo with any improvement in sperm quality. The pH is usually slightly alkaline in chronic bacterial prostatitis with an average pH of 8.32 [4]. The sperm function is altered not only at the testicular level but also at the accessory gland level resulting in infertility.…”

Section: Discussionmentioning

confidence: 99%

Infection and Male Infertility: Effect of Different Antibiotic Regimens on Semen Quality

Merino¹,

Carranza-Lira²

1995

Archives of Andrology

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Objective criteria of semen quality were evaluated in 60 men with positive culture of semen after treatment with two different antibiotics and compared with placebo. Group I received 160 mg trimethoprim and 800 mg sulfamethoxazole twice a day, group I1 received 250 mg ciprofloxacin twice a day, and group 111 received a placebo. No difference was recorded between antibiotic treatment and placebo in sperm quality but accessory gland function was modified. s e m e n infection, male infertility, antibiotic Keywords

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“…Unfortunately, few antibiotics have these necessary characteristics to be of use in the treatment of chronic prostatitis. In addition, some studies have indicated that the pH of prostatic fluid in CBP patients is alkaline rather than acidic [22, 23, 24]. The fluoroquinolones have an advantage in this respect because they are amphoteric drugs or zwitterions and thus have two pKa values, one at acid pH and one at alkaline pH.…”

Section: Principles Of Antibiotic Treatment In Chronic Prostatitismentioning

confidence: 99%

The Role of Antibiotics in the Treatment of Chronic Prostatitis:A Consensus Statement

et al. 1998

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Practical guidelines for the diagnosis and treatment of chronic prostatitis are presented. Chronic prostatitis is classified as chronic bacterial prostatitis (culture-positive) and chronic inflammatory prostatitis (culture-negative). If chronic bacterial prostatitis is suspected, based on relevant symptoms or recurrent UTIs, underlying urological conditions should be excluded by the following tests: rectal examination, midstream urine culture and residual urine. The diagnosis should be confirmed by the Meares and Stamey technique. Antibiotic therapy is recommended for acute exacerbations of chronic prostatitis, chronic bacterial prostatitis and chronic inflammatory prostatitis, if there is clinical, bacteriological or supporting immunological evidence of prostate infection. Unless a patient presents with fever, antibiotic treatment should not be initiated immediately except in cases of acute prostatitis or acute episodes in a patient with chronic bacterial prostatitis. The work-up, with the appropriate investigations should be done first, within a reasonable time period which, preferably, should not be longer than 1 week. During this period, nonspecific treatment, such as appropriate analgesia to relieve symptoms, should be given. The minimum duration of antibiotic treatment should be 2–4 weeks. If there is no improvement in symptoms, treatment should be stopped and reconsidered. However, if there is improvement, it should be continued for at least a further 2–4 weeks to achieve clinical cure and, hopefully, eradication of the causative pathogen. Antibiotic treatment should not be given for 6–8 weeks without an appraisal of its effectiveness. Currently used antibiotics are reviewed. Of these, the fluoroquinolones ofloxacin and ciprofloxacin are recommended because of their favourable antibacterial spectrum and pharmacokinetic profile. A number of clinical trials are recommended and a standard study design is proposed to help resolve some outstanding issues.

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The Response of Prostatic Fluid pH in Inflammation

Cited by 79 publications

References 7 publications

Development of the First Fluorescence Screening Assay for the SLC39A2 Zinc Transporter

Development of the First Fluorescence Screening Assay for the SLC39A2 Zinc Transporter

Infection and Male Infertility: Effect of Different Antibiotic Regimens on Semen Quality

The Role of Antibiotics in the Treatment of Chronic Prostatitis:A Consensus Statement

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