The response of lymphatic smooth muscles to vasoactive substances

Ohhashi, Toshio; Kawai, Yasuaki; Azuma, Takehiko

doi:10.1007/bf00584242

Cited by 90 publications

(53 citation statements)

References 30 publications

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“…When tone was reduced, Jl did not change with isoprenaline, decreased with pentobarbitone or with isotonic solutions of MgSO4, mannitol, or glucose as the serosal fluid (Table 2), but appreciably increased with noradrenaline and adrenaline. The increase of Jl by the latter could be related to their effects on the increases in mesenteric lymphatic contractility (Florey, 1927;Ohhashi, Kawai & Azuma, 1978;Mawhinney & Roddie, 1973) which may promote lymph flow. When the luminal fluid was isotonic MgSO4 or LiCl solution, fluid was secreted into the lumen of the intestine and motility was increased to 2-3 +, but Jl was 0 in both cases.…”

Section: Discussionmentioning

confidence: 99%

Relationship between intestinal motility, tone, water absorption and lymph flow in the rat.

Lee¹

1983

The Journal of Physiology

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SUMMARY1. The relations between motility, tone, water absorption rate (J,) and lymph flow (J,), were studied in an exteriorized upper jejunal preparation in vivo, with its serosal side bathed in Krebs-Ringer or other solutions. Neither J, nor J, was correlated with motility.2. When the luminal fluid consisted of an isotonic solution of MgSO4, glucose or LiCl, motility was greatly increased with no increase in J1.3. When the serosal fluid consisted of an isotonic solution of mannitol, glucose or MgSO4, motility was depressed with a large decrease in tone, and J, was increased by 51-83 % with a decrease in J,. When the serosal fluid consisted of an isotonic solution of NaCl, Na2SO4, LiCl or KCl, motility was decreased with either no change or an increase in tone, and both J, and J, were much decreased. When isotonic cholihe Cl solution was employed as the serosal fluid, motility increased with an increase in tone, J, became negative (fluid secretion), and J, decreased to 0. 4. Acetylcholine depressed motility but increased tone with a decrease in Jv and J,. Eserine increased both tone and motility with a decrease in Jv and Ji. Serotonin caused no change in motility but increased tone with a decrease in J4 and J,.Noradrenaline and adrenaline depressed motility and reduced tone with an increase in Jv and Ji. Isoprenaline abolished motility and reduced tone with a large increase in Jv but no change in J,. Pentobarbitone depressed motility and reduced tone with an increase in Jv but a decrease in J,.5. It is concluded that the intestine responds to various stimuli with a change in motility, or tone, or both. Intestinal motility per se does not produce lymph. Since an increase or decrease in tone always results in a decrease or increase in J, respectively, regardless of the nature of the conditions, a relaxation of the intestinal musculature appears to be associated with an increase in water absorption.

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Section: Discussionmentioning

confidence: 99%

Relationship between intestinal motility, tone, water absorption and lymph flow in the rat.

Lee¹

1983

The Journal of Physiology

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“…ATP increased the frequency of spontaneous contractions of lymphatic smooth muscles in sheep (13) and guinea pigs (7,50). In contrast, isolated and precontracted ring preparations of bovine (33), porcine (10), and canine (41) lymph vessels caused relaxation in response to ATP. Whether ATP can decrease intrinsic pump activity of lymph vessels has not been resolved.…”

Section: Atp-sensitive Kmentioning

confidence: 94%

ATP inhibits pump activity of lymph vessels via adenosine A₁ receptor-mediated involvement of NO- and ATP-sensitive K⁺ channels

Kousai¹,

Mizuno²,

Ikomi³

et al. 2004

American Journal of Physiology-Heart and Circulatory Physiology

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Kousai, Akira, Risuke Mizuno, Fumitaka Ikomi, and Toshio Ohhashi. ATP inhibits pump activity of lymph vessels via adenosine A 1 receptor-mediated involvement of NO-and ATP-sensitive K ϩ channels. Am J Physiol Heart Circ Physiol 287: H2585-H2597, 2004. First published August 12, 2004 doi:10.1152/ajpheart.01080. 2003.-We examined the effects of ATP on intrinsic pump activity in lymph vessels isolated from the rat. ATP caused significant dilation with a cessation of lymphatic pump activity. Removal of the endothelium or pretreatment with N -nitro-L-arginine methyl ester (L-NAME) significantly reduced ATP-induced inhibitory responses of lymphatic pump activity, whereas reduction was not suppressed completely by 10 Ϫ6 M ATP. L-Arginine significantly restored ATPinduced inhibitory responses in the presence of L-NAME. ATPinduced inhibitory responses in lymph vessels with endothelium were also significantly, but not completely, suppressed by pretreatment with glibenclamide. 8-Cyclopentyl-1,3-dipropylxanthine (a selective adenosine A 1 receptor antagonist), but not suramine (a P2X and P2Y receptor antagonist) or 3,7-dimethyl-1-proparglyxanthine (a selective adenosine A 2 receptor antagonist), significantly decreased ATP-induced inhibitory responses. ␣,␤-Methylene ATP (a selective P2X and P2Y receptor agonist) had no significant effect on lymphatic pump activity. In some lymph vessels with endothelium (24 of 30 preparations), adenosine also caused dose-dependent dilation with a cessation of lymphatic pump activity. L-NAME significantly reduced the inhibitory responses induced by the lower (3 ϫ 10 Ϫ8 -3 ϫ 10 Ϫ7 M) concentrations of adenosine. Glibenclamide or 8-cyclopentyl-1,3-dipropylxanthine also significantly suppressed adenosine-induced inhibitory responses. These findings suggest that ATP-induced dilation and inhibition of pump activity of isolated rat lymph vessels are endothelium-dependent and -independent responses. ATP-mediated inhibitory responses may be, in part, related to production of endogenous nitric oxide, involvement of ATP-sensitive K ϩ channels, or activation of adenosine A 1 receptors in lymphatic smooth muscle and endothelium.rat; endothelium; smooth muscle; nitric oxide THE TRANSPORT OF LYMPH depends on passive and active driving forces as well as the rate of lymph production in organs and tissues. The active driving forces, resulting from intrinsic pump activity of lymph vessels, play a pivotal role in the centripetal propulsion of lymph (1,31,39). Neural (21), humoral (30), and mechanical factors (8,22,23) modify the rhythm and amplitude of lymphatic intrinsic pump activity. Recently, it has become clear that lymphatic endothelium-derived nitric oxide (NO) strongly contributes to regulation of lymphatic intrinsic pump activity. Thus NO causes relaxation of lymphatic smooth muscles and reduces lymphatic intrinsic pump activity (24,26,27,35,40,45,48). ATP-sensitive K ϩ (K ATP ) channels have been found in the plasma membrane of cells, including vascular and nonvascular smooth muscles, and then beca...

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“…7,42 Extending the cardiac pump analogy, the intrinsic lymph pump can be modulated via inotropic (i.e., changes in the strength of contraction) and=or chronotropic (i.e., mediated by changes in the contraction frequency) fashions and physical, neural, and humoral influences. 4,6,25,39,50,56,63,65,67,[90][91][92][93][94][95][96][97][98] Our laboratory group has focused much of our efforts analyzing the physical effects of stretch and shear on the intrinsic lymph pump and other contractile mechanisms. Elevated lymph pressure acting via an increase in the stretch of the lymphatic vessel is a classic activator of the lymph pump.…”

Section: Lymphatic Contractility and The Intrinsic Lymph Pumpmentioning

confidence: 99%

Contractile Physiology of Lymphatics

Zawieja¹

2009

Lymphatic Research and Biology

292

307

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The response of lymphatic smooth muscles to vasoactive substances

Cited by 90 publications

References 30 publications

Relationship between intestinal motility, tone, water absorption and lymph flow in the rat.

Relationship between intestinal motility, tone, water absorption and lymph flow in the rat.

ATP inhibits pump activity of lymph vessels via adenosine A₁ receptor-mediated involvement of NO- and ATP-sensitive K⁺ channels

Contractile Physiology of Lymphatics

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The response of lymphatic smooth muscles to vasoactive substances

Cited by 90 publications

References 30 publications

Relationship between intestinal motility, tone, water absorption and lymph flow in the rat.

Relationship between intestinal motility, tone, water absorption and lymph flow in the rat.

ATP inhibits pump activity of lymph vessels via adenosine A1 receptor-mediated involvement of NO- and ATP-sensitive K+ channels

Contractile Physiology of Lymphatics

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ATP inhibits pump activity of lymph vessels via adenosine A₁ receptor-mediated involvement of NO- and ATP-sensitive K⁺ channels