The response of human ectomesenchymal dental pulp stem cells to cisplatin treatment

Seifrtová, Martina; Havelek, Radim; Ćmielová, Jana; Jiroutová, Alena; Soukup, Tomáš; Brůčková, Lenka; Mokrý, Jaroslav; English, Denis; Řezáčová, Martina

doi:10.1111/j.1365-2591.2011.01990.x

Cited by 38 publications

(23 citation statements)

References 33 publications

(62 reference statements)

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“…EMSCs have been successfully isolated and well characterized from the fetal facial and palatal processes, first branchial arch of embryo [Deng et al, 2004], dental pulp [Seifrtova et al, 2012], and even from the olfactory mucosa [Delorme et al, 2010]. Since nasal respiratory mucosa and olfactory mucosa are continuous and seem to have the same origin, success in harvesting the EMSCs from the nasal respiratory mucosa is unsurprising.…”

Section: Discussionmentioning

confidence: 99%

Fibrin Scaffolds Containing Ectomesenchymal Stem Cells Enhance Behavioral and Histological Improvement in a Rat Model of Spinal Cord Injury

Liu

Chen

Zhang

et al. 2013

Cells Tissues Organs

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Fibrin has been widely used in wound healing. However, its benefit for spinal cord injury (SCI) is limited. In this study, we investigated the impact of fibrin scaffolds containing ectomesenchymal stem cells (EMSCs) on histological and behavioral recovery after SCI and compared it with fibrin alone. To achieve this, EMSCs derived from adult rat nasal respiratory mucosa were cultured, characterized and transfected with green fluorescent protein adenovirus before transplantation. Then, Sprague-Dawley host rats were randomly assigned into four groups: the control group (laminectomy); the SCI group (laminectomy and transection of spinal cords); the fibrin group (fibrin was transplanted immediately after SCI), and the fibrin cell (FC) group (fibrin scaffolds containing EMSCs were transplanted after SCI). Three days after the operation, a TUNEL assay indicated less apoptotic cells in the FC group than in the fibrin group. Two weeks after SCI, fluorescence staining demonstrated not only the survival and migration of EMSCs into the lesion sites, but also a higher number of nerve fibers in the FC group than in the fibrin group. Histological examination including immunohistochemistry and transmission electron microscopy 12 weeks after the operation showed more nerve fibers and a thicker myelin sheath in the FC group compared to the fibrin group. Western blotting confirmed these morphological results. Consistent with the histological results, Basso, Beattie and Bresnahan locomotor scores of the FC group were higher than those of the fibrin group. These results suggest that fibrin scaffolds containing EMSCs can improve the behavioral and histological recovery after SCI better than fibrin alone.

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Section: Discussionmentioning

confidence: 99%

Fibrin Scaffolds Containing Ectomesenchymal Stem Cells Enhance Behavioral and Histological Improvement in a Rat Model of Spinal Cord Injury

Liu

Chen

Zhang

et al. 2013

Cells Tissues Organs

View full text Add to dashboard Cite

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“…На повреждение ДНК клетки реагируют активацией внутриклеточных каскадов реакций, приводящих к репарации ДНК или к остановке клеточного цикла и апоптозу [4]. Белок р53, главный супрессор опухолевых клеток, являясь регуля-тором клеточного цикла и апоптоза, играет ключевую роль в чувствительности к CPt.…”

Section: Discussionunclassified

“…Экспрессия р21, в свою очередь, ингибирует действие циклинзави-симых киназ 1-го и 2-го типа (Cdk1 и Cdk2), вызывая тем самым остановку клеточного цикла. В том случае, если повреждения ДНК непоправимы, р53 запускает гибель клетки по пути апоптоза [4].…”

Section: Discussionunclassified

“…Акти-вация каскадов с участием с-Jun-N-концевой киназы (JNK) и р38 запускает проапоптотические реакции, тогда как каскад, опосредованный ERK1/2, связан с пролиферацией клеток и антиапоптотическим про-цессом. Активация тех или иных MAPK является спец-ифичной для типа клеток и зависит от сложной сети сигнальных путей внутри них [4].…”

Section: Discussionunclassified

“…Исследования, посвященные оценке устойчиво-сти ММСК к различным ДНК-повреждающим агентам, являются крайне актуальными, так как химиотерапия остается основным методом лечения для большинства типов рака. Цисплатин (CPt) (цис-диамин-дихлор-платина) -один из самых мощных химиотерапевтиче-ских агентов, используемых против широкого спектра злокачественных опухолей, в том числе головы и шеи, семенников, яичников, мочевого пузыря и легких [4]. Тем не менее его положительные эффекты ограничены из-за токсического действия на нетрансформированные здоровые ткани организма [5].…”

Section: обоснованиеunclassified

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The effects of cysplatin on human adipose tissue derived mesenchymal stromal cells under different oxygen levels

2016

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Isolation, cultivation, and characterization of human mesenchymal stem cells

et al. 2017

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Mesenchymal stem cells (MSC) exhibit a high self-renewal capacity, multilineage differentiation potential and immunomodulatory properties. This set of exceptional features makes them an attractive tool for research and clinical application. However, MSC are far from being a uniform cell type, which makes standardization difficult. The exact properties of human MSC (hMSC) can vary greatly depending on multiple parameters including tissue source, isolation method and medium composition. In this review we address the most important influence factors. We highlight variations in the differentiation potential of MSC from different tissue sources. Furthermore, we compare enzymatic isolation strategies with explants cultures focusing on adipose tissue and umbilical cords as two relevant examples. Additionally, we address effects of medium composition and serum supplementation on MSC expansion and differentiation. The lack of standardized methods for hMSC isolation and cultivation mandates careful evaluation of different protocols regarding efficiency and cell quality. MSC characterization based on a set of minimal criteria defined by the International Society for Cellular Therapy is a widely accepted practice, and additional testing for MSC functionality can provide valuable supplementary information. The MSC secretome has been identified as an important signaling mechanism to affect other cells. In this context, extracellular vesicles (EVs) are attracting increasing interest. The thorough characterization of MSC-derived EVs and their interaction with target cells is a crucial step toward a more complete understanding of MSC-derived EV functionality. Here, we focus on flow cytometric approaches to characterize free as well as cell bound EVs and address potential differences in the bioactivity of EVs derived from stem cells from different sources. © 2017 International Society for Advancement of Cytometry.

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The response of human ectomesenchymal dental pulp stem cells to cisplatin treatment

Abstract: Dental pulp stem cells had a greater genotoxic stress response to cisplatin compared to HDFs. Cisplatin in higher concentrations triggered activation of MAPK and apoptosis in DPSCs but not in HDFs.

Cited by 38 publications

References 33 publications

Fibrin Scaffolds Containing Ectomesenchymal Stem Cells Enhance Behavioral and Histological Improvement in a Rat Model of Spinal Cord Injury

Fibrin Scaffolds Containing Ectomesenchymal Stem Cells Enhance Behavioral and Histological Improvement in a Rat Model of Spinal Cord Injury

The effects of cysplatin on human adipose tissue derived mesenchymal stromal cells under different oxygen levels

Isolation, cultivation, and characterization of human mesenchymal stem cells

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